Neurophysiological studies of Heteropteris glabra Hok. &amp; Arn. (Malpighiaceae) in DBA/2J mice

Galietta, Gabriella; Giuliani, Giusi; Loizzo, Alberto; Amat, Anibal Gumersindo; Fumagalli, Emiliano; Feo, Vincenzo De; Quaranta, Emilia; Paladino, Lidia; Capasso, Anna

doi:10.1016/j.jep.2004.12.003

Cited by 8 publications

(6 citation statements)

References 7 publications

(5 reference statements)

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“…Thus, to evaluate the CNF1 effects against seizures generation, we analyzed the HFOs that have been implicated in many forms of seizures (spikes, poly-spikes, spike-and-slow wave sequences) [ 45 , 46 ] and that are expressed in aged D2 mice [ 38 ]. In fact, HFOs are preferentially localized in the brain region generating spontaneous seizures, and have been considered a potential biomarker of epileptogenic brain [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The present study was designed to explore the effects of the Rho GTPases’ modulator CNF1 on deputy biomarkers for seizures in the the neocortex. Particularly, we investigated the CNF1 effect on energy homeostasis and brain plasticity in the inbred strain of mice DBA/2J, a multipurpose neurological disease model that presents high susceptibility to induced [ 26 – 28 ] or spontaneous seizures [ 29 – 33 ], and the presence of spontaneous fast ripples [ 38 ]. We herein report that a single icv injection of CNF1 is able to raise a consistent and enduring antagonism to the spontaneous cortical EEG SWDs and HFOs (100–500 Hz range) during the physiological state of wakefulness in six-month-old DBA/2J mice.…”

Section: Discussionmentioning

confidence: 99%

“…EEG spectrograms were also elaborated according to a protocol of time and frequency-domain analysis, and parameters set up and published from our laboratories [ 38 , 39 ]. Briefly, spectrograms were recalled on the display, and elaborated according to the Soundscope protocol, in blocks of 600s.…”

Section: Methodsmentioning

confidence: 99%

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CNF1 Enhances Brain Energy Content and Counteracts Spontaneous Epileptiform Phenomena in Aged DBA/2J Mice

et al. 2015

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Epilepsy, one of the most common conditions affecting the brain, is characterized by neuroplasticity and brain cell energy defects. In this work, we demonstrate the ability of the Escherichia coli protein toxin cytotoxic necrotizing factor 1 (CNF1) to counteract epileptiform phenomena in inbred DBA/2J mice, an animal model displaying genetic background with an high susceptibility to induced- and spontaneous seizures. Via modulation of the Rho GTPases, CNF1 regulates actin dynamics with a consequent increase in spine density and length in pyramidal neurons of rat visual cortex, and influences the mitochondrial homeostasis with remarkable changes in the mitochondrial network architecture. In addition, CNF1 improves cognitive performances and increases ATP brain content in mouse models of Rett syndrome and Alzheimer's disease. The results herein reported show that a single dose of CNF1 induces a remarkable amelioration of the seizure phenotype, with a significant augmentation in neuroplasticity markers and in cortex mitochondrial ATP content. This latter effect is accompanied by a decrease in the expression of mitochondrial fission proteins, suggesting a role of mitochondrial dynamics in the CNF1-induced beneficial effects on this epileptiform phenotype. Our results strongly support the crucial role of brain energy homeostasis in the pathogenesis of certain neurological diseases, and suggest that CNF1 could represent a putative new therapeutic tool for epilepsy.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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CNF1 Enhances Brain Energy Content and Counteracts Spontaneous Epileptiform Phenomena in Aged DBA/2J Mice

et al. 2015

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“…improved learning and memory deficits in aged rats [23], and has also showed a strong reduction in the oxidative stress in young and old rat brains [24], whereas the species Heteropterys glabra Hook & Arn. induced a reduction in motor activity and alterations in EEG parameters [25]. Recently, our work group described the antidepressant, anxiolytic, and anticonvulsant activities in mice without toxicity effects of the methanolic extract of Heteropterys brachiata (L.) DC.…”

Section: Resultsmentioning

confidence: 99%

Heteropterys cotinifolia: A Neuropharmacological and Phytochemical Approach with Possible Taxonomic Implications

Huerta-Reyes

Zamilpa

Álvarez-Chimal

et al. 2013

The Scientific World Journal

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Heteropterys cotinifolia (Malpighiaceae) has been used in traditional Mexican medicine mainly for the treatment of nervous disorders. However, the specific neuropharmacological activities responsible for this use remain to be defined. The present study evaluates the antidepressant and anxiolytic effects produced by the methanolic extract of Heteropterys cotinifolia and the influence of such effects on motor activity in ICR mice. Our results show that the methanolic extract of Heteropterys cotinifolia produces a dose-dependent antidepressant effect in the forced swimming test in mice at doses from 31 to 310 mg/kg, with no reduction of mice locomotion. However, no anxiolytic properties were observed. Our findings suggest that the main extract compounds identified as chlorogenic acid and rutin may be involved in the antidepressant effects. To our knowledge, the present study constitutes the first report of pharmacological and phytochemical data of Heteropterys cotinifolia. The presence of flavonoids in the methanolic extract of Heteropterys cotinifolia may also provide further data to characterize taxonomically this species in order to be distinguished from others species closely related and belonging to the same genus.

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“…We also performed the first phytochemical profile of this plant species. Several studies have been conducted with polar extracts and compounds isolated from plants of the Malpighiaceae family and have shown CNS-level anxiolytic, antidepressant, hypnotic, anticonvulsant, and antischizophrenic effects in several pharmacological tests [4,17,[32][33][34][35][36][37]. Some chemical compounds have been proposed to be responsible for these pharmacological effects.…”

Section: Discussionmentioning

confidence: 99%

Neuropharmacological Activity of the Acetonic Extract of Malpighia mexicana A. Juss. and Its Phytochemical Profile

Avilés-Montes,

Salinas-Sánchez,

Sotelo-Leyva

et al. 2023

Sci. Pharm.

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Mental and neurological disorders are conditions that affect thoughts, emotions, behavior, and relationships. Malpighia mexicana A. Juss. is a plant used in Mexican traditional medicine for the treatment of such disorders. This work aimed to investigate the antidepressant, anxiolytic, sedative, hypnotic, and anticonvulsant effects of the acetonic extract (MmAE) of M. mexicana and its fractions (F3, F4-10, F14) using the forced swimming test (FST), elevated plus maze (EPM), open field test (OFT), pentobarbital-induced sleep test (PBTt), and pentylenetetrazol-induced seizure test (PTZt). MmAE, F3, F4-10, F14, and vehicle were administrated orally 24, 18, and 1 h prior to the evaluations. Imipramine (15 mg/kg, p.o.) was administrated 1 h prior to the evaluations as a positive control for the FST, while diazepam (1 mg/kg, p.o.) was administrated 1 h prior to the evaluations as a positive control for the EPM, OFT, PBTt, and PTZt. MmAE had an anxiolytic effect; MmAE and F3, F4-10, and F14 showed an antidepressant effect, sedative effect, hypnotic effect, and anticonvulsant effect. Using HPLC, we identified the compounds quercetin 3-O-rutinoside (1), kaempferol 3-O-glucoside (2), luteolin 7-O-glucoside (3), quercetin (4), and kaempferol (5) in MmAE and compounds (1), (2), and (3) in F14. Using GC-MS, we identified α-tocopherol, phytol, and β-amyrin in F3; β-tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10; and α- tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10. The neuropharmacological effects found in this work may be due to the presence of vitamins, phytosterols, terpenes, and flavonoids. This research requires further study to clarify the mechanisms of action of the identified compounds.

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Neurophysiological studies of Heteropteris glabra Hok. & Arn. (Malpighiaceae) in DBA/2J mice

Cited by 8 publications

References 7 publications

CNF1 Enhances Brain Energy Content and Counteracts Spontaneous Epileptiform Phenomena in Aged DBA/2J Mice

CNF1 Enhances Brain Energy Content and Counteracts Spontaneous Epileptiform Phenomena in Aged DBA/2J Mice

Heteropterys cotinifolia: A Neuropharmacological and Phytochemical Approach with Possible Taxonomic Implications

Neuropharmacological Activity of the Acetonic Extract of Malpighia mexicana A. Juss. and Its Phytochemical Profile

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