Neuronal localization of the 25-kDa specific thiamine triphosphatase in rodent brain

Czerniecki, Jan; Chanas, Grazyna; Verlaet, Myriam; Bettendorff, Lucien; Makarchikov, Alexander F; Leprince, Pierre; Wins, Pierre; Grisar, Thierry; Lakaye, Bernard

doi:10.1016/j.neuroscience.2004.02.033

Cited by 15 publications

(15 citation statements)

References 36 publications

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“…White matter was not significantly labeled. TTPase immunoreactivity seems to be located mainly in the cytoplasm and dendrites (Czerniecki et al 2004). Subcellular structures precipitated as microsomal fractions, e.g., endoplasmic reticulum and vesicular parts of plasma membranes were encrusted with TTPbinding proteins (Ianchii et al 2003).…”

Section: Membrane Sitesmentioning

confidence: 99%

“…Indeed, the membrane-associated enzyme form (TTPase) may play a physiologic role other than TTP hydrolysis in mammalian tissues (Szyniarowski et al 2005). In vertebrate tissues, TTPase may act as a phosphate donor for the phosphorylation of certain proteins; that may be part of a new signal transduction pathway (Czerniecki et al 2004). Thiamine deficiency decreases membrane-associated TTPase activity (Iwata et al 1974).…”

Section: Membrane Sitesmentioning

confidence: 99%

“…Membrane-associated TTPase is affected by proteolytic enzymes (Bettendorff et al 1987). Distribution of TTPase activity, typical of thiamine-binding proteins in cellular structures, was studied (Ianchii et al 2003;Czerniecki et al 2004). TPPase immunocytochemical labeling showed the strongest staining in hippocampal pyramidal neurons, as well as cerebellar granule cells and Purkinje cells.…”

Section: Membrane Sitesmentioning

confidence: 99%

“…However, literature that takes an interest in the structural role of thiamine is growing. So, thiamine interferes with the membrane structure and function (Tanaka and Cooper 1968;Itokawa et al 1972;Goldberg et al 2004), acts against agents-induced cytotoxicity (Bâ et al 1996;Aberle et al 2004) and fixes membrane sites (Czerniecki et al 2004;Spector and Johanson 2007). Those observations illustrate the noncoenzymatic function of the vitamin.…”

Section: Introductionmentioning

confidence: 98%

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Metabolic and Structural Role of Thiamine in Nervous Tissues

Bâ

2008

Cell Mol Neurobiol

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In the literature, previous descriptions of the role of thiamine (B1 vitamin) focused mostly on its biochemical functions as a coenzyme precursor of some key enzymes of the carbohydrate metabolism. This report reviews recent developments on the metabolic and structural role of thiamine, e.g., the coenzyme and noncoenzyme functions of the vitamin. Taking into account analysis of our experimental data relating to the effects of thiamine deficiency on developing central nervous system (CNS) and data available in literature, we seek to establish a clear difference between the metabolic and structural role of thiamine. Our experimental data indicate that the specific and nonspecific effects express two diametrically diverse functions of thiamine in development: the nonspecific effects show up the metabolic consequences of thiamine deficiency resulting in apoptosis and severe cellular deficit; inversely, the specific effects announced the structural consequences of thiamine deficiency, described as cellular membrane damage, irregular and ectopic cells. The review highlights the existence of noncoenzyme functions of this vitamin through its interactions with biological membranes.

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Section: Membrane Sitesmentioning

confidence: 99%

Section: Membrane Sitesmentioning

confidence: 99%

Section: Membrane Sitesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Metabolic and Structural Role of Thiamine in Nervous Tissues

Bâ

2008

Cell Mol Neurobiol

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“…This 25-kDa cytoplasmic protein is a highly efficient ThTPase ubiquitously expressed in adult mammalian tissues. However, it seems to be most 5 abundant in highly differentiated cells while it is hardly detectable in cultured cells, suggesting that the expression of this enzyme is linked to the degree of cellular differentiation 20,21 .…”

Section: The Case Of Thiamine Triphosphatementioning

confidence: 99%