Neuronal gene delivery by negatively charged pullulan–spermine/DNA anioplexes

Thakor, Devang K.; Teng, Yang D.; Tabata, Yasuhiko

doi:10.1016/j.biomaterials.2008.12.032

Cited by 62 publications

(40 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pullulan was chosen to develop anionic polymer and drug conjugation because it has been widely explored for various biomedical applications such as tissue engineering, targeted drug and gene delivery due to its unique biocompatibility profile (Prajapati et al 2013). Pullulan derivatives with various cationic polymers such as protamine, polyethyleneimine, spermine, and glycidyl trimethyl ammonium chloride have been explored for gene delivery to increase hemocompatibility and efficiency of a gene delivery vector component (Priya et al 2014;Rekha and Sharma 2011;Thakor et al 2009;Thomsen et al 2011) Besides that, various targeted pullulandrug conjugates have been investigated for improving efficacy of cytotoxic drugs on tumor site along with fewer side effects on normal tissues (Li et al 2013a, b;Scomparin et al 2011;Wang et al 2013). Carboxylmethylated pullulan (CMP) is an anionic derivative of pullulan, which has been widely explored for biomedical applications (Dulong et al 2012;Pereiraa, et al 2014;Mocanu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

et al. 2014

View full text Add to dashboard Cite

The amalgamation of chemotherapy and gene therapy is promising treatment option for cancer. In this study, novel biocompatible self-assembled nanocomplexes (NCs) between carboxylmethylated pullulan t335 (CMP) with polyallylamine (CMP-PAA NCs) were developed for plasmid DNA (pDNA) and pH-sensitive doxorubicin (DOX) delivery. DOX was conjugated to CMP (DOX-CMP) via hydrazone and confirmed by FTIR and 1 H-NMR. In vitro release studies of pH-sensitive DOX-CMP conjugate showed 23 and 85 % release after 48 h at pH 7.4 (physiological pH) and pH 5 (intracellular/tumoral pH), respectively. The CMP-PAA NCs or DOX-CMP-PAA NCs self-assembled into a nanosized (\250 nm) spherical shape as confirmed by DLS and TEM. The hemolysis and cytotoxicity study indicated that the CMP-PAA NCs did not show cytotoxicity in comparison with plain polyallylamine. Gel retardation assay showed complete binding of pDNA with CMP-PAA NCs at 1:2 weight ratio. CMP-PAA NCs/pDNA showed significantly higher transfection in HEK293 cells compared to PAA/pDNA complexes. Confocal imaging demonstrated successful cellular uptake of DOX-CMP-PAA NCs in HEK293 cells. Thus, NCs hold great potential for targeted pDNA and pH-sensitive intratumoral drug delivery.

show abstract

Section: Introductionmentioning

confidence: 99%

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Further, β-galactosidase expression in COS-7 cells resulting from pullulan nanoparticles is comparable to commercially available Lipofectamine 2000 (Gupta and Gupta 2004). Pullulan in combination with spermine has been used for transfection of pDNA to rat brain endothelial cells and human brain microvascular endothelial cells (Thomsen et al 2011), rat sensory neurons (Thakor et al 2009), and mesenchymal stem cells (Jo et al 2010). …”

Section: Pullulanmentioning

confidence: 96%

Cationic Polymers for the Delivery of Therapeutic Nucleotides

et al. 2015

View full text Add to dashboard Cite

“…Compared to cationic lipoplexes, negatively charged ones should offer advantages of decreased cytotoxicity and increased serum compatibility (Thakor et al, 2009); however, as mentioned before, their cell internalization is problematic. Cell internalization of negatively charged particles often requires the presence of cell-specific ligands (attached to particle surface) for endocytosis to occur.…”

Section: Surface Chargementioning

confidence: 99%