Neuronal cell patterning on a multi-electrode array for a network analysis platform

Suzuki, Masaaki; Ikeda, Kazuhiko; Yamaguchi, Munehiro; Kudoh, Suguru N.; Yokoyama, Keiko; Satoh, Ryota; Ito, Daisuke; Nagayama, Masaichi; Uchida, Tsutomu; Gohara, Kazutoshi

doi:10.1016/j.biomaterials.2013.03.042

Cited by 32 publications

(34 citation statements)

References 51 publications

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“…We cultured cortical cells derived from Wistar rats at embryonic day 17 using a Nerve-Cell Culture System (Sumitomo Bakelite Co., Tokyo, Japan) as described previously (Ito et al, 2010;Yamaguchi et al, 2011;Uchida et al, 2012;Suzuki et al, 2013). Cortices of 2 rats were dissociated into single cells using dissociation solution (Sumitomo Bakelite Co.), then resuspended in Neuron Culture Medium (serum-free conditioned medium from 48-h rat astrocyte confluent cultures based on Dulbecco's modified Eagle's medium [DMEM]/F-12 with N2 supplement; Sumitomo Bakelite; Takeuchi et al, 2005).…”

Section: Cell Culturementioning

confidence: 99%

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

2013

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The aim of this study was to clarify the saturation processes of excitatory and inhibitory synapse densities during the long-term development of cultured neuronal networks. For this purpose, we performed a long-term culture of rat cortical cells for 35 days in vitro (DIV). During this culture period, we labeled glutamatergic and GABAergic synapses separately using antibodies against vesicular glutamate transporter 1 (VGluT1) and vesicular transporter of -aminobutyric acid (VGAT). The densities and distributions of both types of synaptic terminals were measured simultaneously. Observations and subsequent measurements of immunofluorescence demonstrated that the densities of both types of antibody-labeled terminals increased gradually from 7 to 21-28 DIV. The densities did not show a further increase at 35 DIV and tended to become saturated.Triple staining with VGluT1, VGAT, and microtubule-associated protein 2 (MAP2) enabled analysis of the distribution of both types of synapses, and revealed that the densities of the two types of synaptic terminals on somata were not significantly different, but that glutamatergic synapses predominated on the dendrites during long-term culture.However, some neurons did not fall within this distribution, suggesting differences in synapse distribution on target neurons. The electrical activity also showed an initial increase and subsequent saturation of the firing rate and synchronized burst rate during long-term culture, and the number of days of culture to saturation from the initial increase followed the same pattern under this culture condition.

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Section: Cell Culturementioning

confidence: 99%

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

2013

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“…8), 9) 2. Cultured neuronal networks are very useful for studying fundamental mechanisms associated with nervous system development and functioning.…”

Section: Hodgkinmentioning

confidence: 98%

Developmental Process and Synchronized Bursts in Cultured Neuronal Networks

Ito

Gohara

2014

Biophysics

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“…This was far smaller than that observed for the previously reported chemical-binding-based patterning using light irradiation (typically the resolution is over 10 mm). 12,[14][15][16] This property can be advantageous for various bioanalytical elds; for example, it makes the observation of certain cellular phenomena possible in a relatively long time span, such as neural network formation of neurons 32 or muscle cells 33 and nucleus elongation 34 at a single-cell level. Moreover, the simplicity of the developed method promotes such studies in biological laboratories.…”

Section: Performance Evaluationmentioning

confidence: 99%

Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning

Funano

Tanaka

2016

RSC Adv.

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Neuronal cell patterning on a multi-electrode array for a network analysis platform

Cited by 32 publications

References 51 publications

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

Measurement of saturation processes in glutamatergic and GABAergic synapse densities during long-term development of cultured rat cortical networks

Developmental Process and Synchronized Bursts in Cultured Neuronal Networks

Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning

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