Neuron-restrictive Silencer Factor (NRSF) Functions as a Repressor in Neuronal Cells to Regulate the μ Opioid Receptor Gene

Kim, Chun Sung; Hwang, Cheol Kyu; Choi, Hack Sun; Song, Kyu Young; Law, Ping Yee; Wei, Li‐Na; Loh, Horace H.

doi:10.1074/jbc.m403633200

Cited by 82 publications

(71 citation statements)

References 48 publications

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“…Nuclear Extract Preparation-Nuclear extracts were prepared from NS20Y cells as described previously (24). Briefly cells were grown to confluence, harvested, and washed with phosphate-buffered saline.…”

Section: Methodsmentioning

confidence: 99%

A Proteomics Approach for Identification of Single Strand DNA-binding Proteins Involved in Transcriptional Regulation of Mouse μ Opioid Receptor Gene

Choi

Song

Hwang

et al. 2008

Molecular & Cellular Proteomics

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The pharmacological actions of morphine and morphinelike drugs such as heroin are mediated primarily through the opioid receptor. Previously a single strand DNA element of the mouse opioid receptor gene (Oprm1) proximal promoter was found to be important for regulating Oprm1 in neuronal cells. To identify proteins binding to the single strand DNA element as potential regulators for Oprm1, affinity column chromatography with the single strand DNA element was performed using neuroblastoma NS20Y cells followed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. We identified five poly(C)-binding proteins: heterogeneous nuclear ribonucleoprotein (hnRNP) K, ␣-complex proteins (␣CP) ␣CP1, ␣CP2, ␣CP2-KL, and ␣CP3. Binding of these proteins to the single strand DNA element of Oprm1 was sequence-specific as confirmed by supershift assays. In cotransfection studies, hnRNP K, ␣CP1, ␣CP2, and ␣CP2-KL activated the Oprm1 promoter activity, whereas ␣CP3 acted as a repressor. Ectopic expression of hnRNP K, ␣CP1, ␣CP2, and ␣CP2-KL also led to activation of the endogenous Oprm1 transcripts, and ␣CP3 repressed endogenous Oprm1 transcripts. We demonstrate novel roles as transcriptional regulators in

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Section: Methodsmentioning

confidence: 99%

A Proteomics Approach for Identification of Single Strand DNA-binding Proteins Involved in Transcriptional Regulation of Mouse μ Opioid Receptor Gene

Choi

Song

Hwang

et al. 2008

Molecular & Cellular Proteomics

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“…REST was also found to repress the µ-opioid receptor in neuronal cells, and thus may have a role in opium addiction. 54 Similarly, REST was found to repress the serotonin 1A receptor, which is implicated in depression and anxiety. 55 Among nonneuronal tissues, REST was found to be present in normal ventricular myocytes and to repress the expression of multiple fetal cardiac genes.…”

Section: Rest In Other Disordersmentioning

confidence: 99%

REST In Good Times and Bad: Roles In Tumor Suppressor and Oncogenic Activities

2006

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Previously published online as a Cell Cycle E-publication: http://www.landesbioscience.com/journals/cc/abstract.php?id=2982 KEY WORDSREST/NRSF, epigenetic regulation, neuronal differentiation, chromatin, tumor-suppressor activity, oncogenic activity, colon cancer, breast cancer, lung cancer, medulloblastoma ABBREVIATIONS dsRNA double-stranded RNA ES embryonic stem HDAC histone deacetylase NRSE neuron-restrictive silencer element NRSF neuron-restrictive silencer factor NSC neural stem/progenitor cell RE-1 repressor element 1 REST RE-1 silencing transcription factor ACKNOWLEDGEMENTS

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“…Because ischemia activates the transcriptional repressor REST specifically in CA1 (14,36), and MOR-1 is a known REST target (37,38), we assessed the time course of MOR-1 mRNA expression in CA1 and CA3 by quantitative real-time PCR. In control CA1, MOR-1 mRNA expression was high (n ϭ 15).…”

Section: Transient Global Ischemia Is a Neuronal Insult That Induces mentioning

confidence: 99%

Ischemic insults promote epigenetic reprogramming of μ opioid receptor expression in hippocampal neurons

Formisano

Noh

Miyawaki

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

123

103

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Neuron-restrictive Silencer Factor (NRSF) Functions as a Repressor in Neuronal Cells to Regulate the μ Opioid Receptor Gene

Cited by 82 publications

References 48 publications

A Proteomics Approach for Identification of Single Strand DNA-binding Proteins Involved in Transcriptional Regulation of Mouse μ Opioid Receptor Gene

A Proteomics Approach for Identification of Single Strand DNA-binding Proteins Involved in Transcriptional Regulation of Mouse μ Opioid Receptor Gene

REST In Good Times and Bad: Roles In Tumor Suppressor and Oncogenic Activities

Ischemic insults promote epigenetic reprogramming of μ opioid receptor expression in hippocampal neurons

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