Neurohypophyseal response to fluid resuscitation with hypertonic saline during septic shock in rats

Santiago, Michael Brian; Vieira, Alexandre Antonio; Elias, Lucila Leico Kagohara; Rodrigues, Josemar; Giusti‐Paiva, Alexandre

doi:10.1113/expphysiol.2012.066241

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…The cecum was then isolated, ligated with a 3-0 silk ligature just distal to the ileocecal valve, punctured 10 times at opposite ends with a 16-gauge needle, and returned into the abdominal cavity. All of these animals received a subcutaneous injection of isotonic saline (1 mL·(100 g body mass) −1 ) at the end of the surgery to restore the liquid that the animals lose during the abdominal incision and CLP (Torres-Dueñas et al 2006;Santiago et al 2013). MAP of the rats was monitored during the following 24 h. The sham-operated (sham) rats underwent the same surgical procedure except that the cecum was neither ligated nor punctured (Santiago et al 2013).…”

Section: Arterial Blood Pressure and Heart Rate Recording During Clp-mentioning

confidence: 99%

“…Given that autonomic dysfunction contributes to severe cases of systemic inflammatory response syndrome, disturbances in autonomic-inflammatory interactions may pose additional risks. Specifically, dysfunctions in neurally mediated organ interactions during sepsis, combined with the noninfectious inflammatory response syndrome, may mutually contribute to the development of multiple organ dysfunction syndrome (Wichterman et al 1980;Sayk et al 2008;Giusti-Paiva and Santiago 2010;Santiago et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Impaired chemoreflex sensitivity during septic shock induced by cecal ligation and perforation

Santiago

Vieira

Giusti‐Paiva

2013

Can. J. Physiol. Pharmacol.

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In this study, we sought to determine the effects produced by cecal ligation and perforation (CLP) on the autonomic responses to the activation of peripheral chemoreflexes in conscious rats. The peripheral chemoreflex was activated with potassium cyanide (KCN; 40 μg·(0.1 mL)(-1); intravenous injection (i.v.)) in male Wistar rats 3, 6, 12, and 24 h after CLP or sham surgery. The mean arterial pressure (MAP), heart rate (HR), and respiratory frequency (fR) were recorded simultaneously. CLP surgery reduced the baseline MAP when compared with the sham animals. In the animals of the sham group, the autonomic responses to KCN produced increases in MAP and fR as well as a decrease in HR. However, 12 and 24 h after CLP surgery, the autonomic responses to KCN were attenuated. The restoration of MAP by i.v. injected l-NAME or phenylephrine did not restore the autonomic response to KCN in rats subjected to CLP. These data show that septic shock induced by CLP compromised the autonomic responses to peripheral chemoreflex activation in conscious rats, suggesting that an important regulatory mechanism is impaired during the course of this condition.

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Section: Arterial Blood Pressure and Heart Rate Recording During Clp-mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impaired chemoreflex sensitivity during septic shock induced by cecal ligation and perforation

Santiago

Vieira

Giusti‐Paiva

2013

Can. J. Physiol. Pharmacol.

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“…Caspase-mediated oxidative stress leads to lung apoptosis in severe lung injury by sepsis [6]. Sepsis, among other complications, promotes ventilatory dysfunction [7] as well as impairment on the cardiorespiratory responses to chemoreflex activation in awake rats [8]. These changes are associated with the increase of inflammatory cytokines in bronchoalveolar lavage (BAL) as interferon (IFN)-gamma, Interleucin-1β, interleukin-6 and tumor necrosis factor alpha (TNF-alpha) [9], and its inflammatory balance is related to the severity and mortality of murine sepsis [9].…”

Section: Introductionmentioning

confidence: 99%

Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis

et al. 2019

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Respiratory infection can be exacerbated by the high glucose concentration in the airway surface liquid (ASL). We investigated the effects of salbutamol and phlorizin on the pulmonary function, oxidative stress levels and SGLT1 activity in lung, pulmonary histopathological damages and survival rates of rats with sepsis. Sepsis was induced by cecal ligation and puncture surgery (CLP). Twenty-four hours after surgery, CLP rats were intranasally treated with saline, salbutamol or phlorizin. After 2 hours, animals were anesthetized and sacrificed. Sepsis promoted atelectasis and bronchial inflammation, and led to increased expression of SGLT1 on cytoplasm of pneumocytes. Salbutamol treatment reduced bronchial inflammation and promoted hyperinsuflation in CLP rats. The interferon-ɤ and Interleucin-1β concentrations in bronchoalveolar lavage (BAL) were closely related to the bronchial inflammation regulation. Salbutamol stimulated SGLT1 in plasma membrane; whereas, phlorizin promoted the increase of SGLT1 in cytoplasm. Phlorizin reduced catalase activity and induced a significant decrease in the survival rate of CLP rats. Taken together, sepsis promoted atelectasis and lung inflammation, which can be associated with SGLT1 inhibition. The loss of function of SGLT1 by phlorizin are related to the augmented disease severity, increased atelectasis, bronchial inflammation and a significant reduction of survival rate of CLP rats. Alternatively salbutamol reduced BAL inflammatory cytokines, bronchial inflammation, atelectasis, and airway damage in sepsis. These data suggest that this selective β2-adrenergic agonist may protect lung of septic acute effects.

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“…Vasopressin 1 receptor (V1R) is another player that has an important role in volume resuscitation therapy. It has been determined that in an endotoxic rat model, volume resuscitation can cause vasopressin release to enable the necessary V1R during the periods of low vasopressin secretion for an increased blood pressure in response to the fl uids (37). The same pathway was also active and served a function after volume resuscitation in the CLP rat model of severe sepsis (38).…”

Section: Discussionmentioning

confidence: 99%

Effect of fluid resuscitation on acute lung injury in a rat model of sepsis

Erdoğan¹,

Erdoğan²,

Kara³

et al. 2021

BLL

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AIM: Sepsis is a systemic infection reaction and intravascular volume therapy plays a crucial role in it's treatment. Acute respiratory distress syndrome (ARDS) occurs in the lungs, the most affected organ. This study aimed to investigate the different effects of fl uid therapy on ARDS caused by sepsis. METHOD: To form a sepsis model, cecal ligation and puncture (CLP) procedure were performed on 44 adult rats. Divided into six groups; normal, CLP group, those treated with 40 ml/kg 0.9 % NaCl, 3 % NaCl (hypertonic saline), Ringer Lactate and Hydroxyethyl starch. After 24 hours treatments, histopathological examination of the lungs were done, and the plasma levels of CRP, TNF-α and IL-6 and paO 2 were measured. RESULTS: The scores of all histological parameters of the group treated with hypertonic saline were signifi cantly lower than of the other groups (p < 0.001). Likewise, according to the arterial blood gas results, paO 2 was signifi cantly higher (p < 0.01) in the hypertonic saline group compared to the other groups, and paCO 2 was signifi cantly lower (p < 0.01). CRP, TNF-α and IL-6 levels of infl ammatory markers were also signifi cantly lower in hypertonic saline groups compared to other groups (p < 0.001). CONCLUSIONS: Our study shows that treatment with hypertonic saline reduces the progression of ARDS in sepsis (Tab.

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Neurohypophyseal response to fluid resuscitation with hypertonic saline during septic shock in rats

Cited by 6 publications

References 33 publications

Impaired chemoreflex sensitivity during septic shock induced by cecal ligation and perforation

Impaired chemoreflex sensitivity during septic shock induced by cecal ligation and perforation

Effects of salbutamol and phlorizin on acute pulmonary inflammation and disease severity in experimental sepsis

Effect of fluid resuscitation on acute lung injury in a rat model of sepsis

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