Neurohypophyseal Hormone-Responsive Adenylate Cyclase from Mammalian Kidney

Douša, Thomas P.; Hechter, Oscar; Schwartz, Irving L.; Walter, Roderich

doi:10.1073/pnas.68.8.1693

Cited by 47 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our experiments were performed on piglets in vivo because there exist corresponding studies in vitro on preparations from the same animal (4,15,20). With respect to their small blood volume, ADH cannot be determined directly.…”

Section: Discussionmentioning

confidence: 99%

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

et al. 1979

View full text Add to dashboard Cite

SummaryIn 16 anesthetized piglets, the effects of I-deamino-8-D-arginine-vasopressin (DDAVP) upon urinary flow rate, relative urinary osrilolarity (U/P,,,,,,I), fractional water excretion (U/Pl,), fractional urca excretion (U/P,,,.,,/U/PI,), renal adenosine 3',5'-cyclic nlonophosphate (c-AMP) formation rate, and medullary c-AMP content were studied during the first 6 days of life. Glomerular filtration rate (GFR) was 0.19 + 0.09 ml/min/g kidney wt (means + SD) on day 1 and increased to 0.51 + 0.11 ml/min/g kidney wt on day 6. It was not affected by the application of 20 pg DDAVP/ kg body wt. On day 1, U/P,,,,,1(1.81 + 0.29), urinary flow rate (5.36 + 1.49 pl/n~in/g kidney wt), U/PI, (31.67 + 6.10) and U/ P,,,.,,/U/PI, (0.84 + 0.12) were not affected by DDAVP application. Fro111 day 2 onward, the reponse of these variables to application of DDAVP increased progressively. On day 6, the following responses were observed: Urinary flow rate decreased from 13.85 f. 7.02 pl/nlin/g kidney wt before DDAVP to 5.07 ? 2.19 pl/min/ g kidney wt after DDAVP. U/P,,,,I raised from 1.24 + 0.27 before DDAVP to 2.64 ? 0.17 after DDAVP. U/PI, increased fro111 36.85 + 13.11 before DDAVP to 109.39 + 22.00 after DDAVP. U/P,,,,,/U/P,, decreased from 0.75 ? 0.05 before DDAVP to 0.55 + 0.04 after DDAVP. These data indicate that the snlall solute permeability of the medullary collecting ducts becon~es increasingly sensitive to antidiuretic hormone (ADII) during this period. The increase in responsiveness of the urinary concentrating system with age was paralleled by the effect of DDAVP on renal c-AMP fornlation rate. In the diuretic control state, no correlation between renal c-AMP fornlation rate and the number of days was observed. llowever, after the application of DDAVP, the nephrogenic fraction of renal c-AMP excretion rate increased from 10.58 + 4.49 pnlole/min/g kidney wt on day 1 to 21.61 + 8.10 pmole/ n~in/g kidney wt on day 6. Similar results were observed with the nledullary c-AMP content. In diuretic control animals which had riot received DDAVP, the medullary c-AMP content ranged between 636 ? 257 pnlole/g wet wt on day 1 and 622 + 75 pmole/g wet wt on day 6. IIowever, in animals which had been infused with DDAVP, niedullary c-AMP content increased from 684 + 274 pnrole/g wet wt on day 1 to 1536 ? 316 pmole/g wet wt on day 6. SpeculationThe unresponsiveness of the medullary adenylatecyclase to AD11 in the newborn kidney might restrict, in addition to other parameters, the renal concentrating ability during the neonatal period.It is generally acknowledged that the concentrating ability of the newborn kidney is reduced in comparison to that of the mature kidney. In the past, this phenomenon was predominately attributed to the structural immaturity of the renal medulla (2, 22), to the reduced G F R and to the anabolic state of the neonatal metabolism, which together result in low filtration-, reabsorption-, and excretion rate of urea (5, 6). It was argued that in spite of the effects of ADH on the permeability of the collecting d...

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

et al. 1979

View full text Add to dashboard Cite

show abstract

“…The known components of the cellular action of vasopressin are qualitatively similar in all mammalian renal medulla examined (15), and bovine renal medulla was chosen for our in vitro studies because it was available in sufficient quantities for subcellular preparations.…”

Section: Discussionmentioning

confidence: 99%

“…When particulate fractions of renal medullary homogenate were studied and compared with soluble proteins, double-layer disks of DEAE cellulose paper and GF/C Whatman glass fiber papers were used to assure retention of membrane fragments. GF/C glass fiber paper itself retained neither [ Adenylate cyclase was assayed by the method described in our previous study (15). For the chromatographic separation of cyclic AMP from other nucleotides, we used a modification described recently (16).…”

Section: Introductionmentioning

confidence: 99%

Effects of Colchicine and Vinblastine on the Cellular Action of Vasopressin in Mammalian Kidney A POSSIBLE ROLE OF MICROTUBULES

Douša¹,

Barnes²

1974

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…This suggests that uptake by the contraluminal aspect of the tubular cells occurs. It is likely that at least part of the AVP cleared from the peritubular compartment binds to specific receptors on the contraluminal plasma membranes of the distal tubule and collecting ducts with subsequent activation of vasopressin-sensitive adenylate cyclase at this site (12)(13)(14)(15). Apart from serving to stimulate the formation of cyclic-AMP, the AVP removed from the peritubular compartment is probably degraded by the rich supply of enzymes present in the plasma membrane (16) and in the cell (17)(18)(19).…”

Section: Perfusion Studiesmentioning

confidence: 99%

The Handling of Immunoreactive Vasopressin by the Isolated Perfused Rat Kidney

Rabkin¹,

Share²,

Payne³

et al. 1979

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Using the isolated rat kidney perfused with an artificial medium containing glucose as the sole fuel, we studied the renal handling of immunoreactive arginine vasopressin (AVP) and determined the effect of various factors on the ability of the kidney to remove AVP.In control kidneys perfused with AVP at concentrations below 116 uU/ml, the organ clearance of AVP (OCAVP) was 1,145±47 (SE) p1l/min, whereas glomerular filtration rate (GFR) averaged 515±37 ,ul/min. Filtration could thus account for up to 45% of the OCAVP, the balance presumably being cleared from the peritubular circulation. Ofthe AVP filtered, only 38% could be recovered in the urine (urinary clearance AVP averaged 205±12 ,p/min) suggesting that the balance was taken up by the tubular epithelium and degraded. Fractional excretion of filtered AVP rose significantly in the presence of anoxia and cold (10°C) to 49 and 59%, respectively, but was not affected by ouabain or high levels of AVP (458±58 ,uU/ml).The OCAVP was not significantly altered by the absence of glucose in the perfusate, anoxia, or ureteral ligation, maneuvers that were associated with significant reductions in GFR. In these and the control experiments, there was a significant inverse correlation between GFR and peritubular clearance emphasizing the importance of the latter (r = -0.749; P < 0.001). Cold, ouabain, and high concentrations ofAVP reduced the clearance of AVP by the kidneys.On the basis of these studies we conclude that the kidney clears AVP from the circulation via two pathPortions of this work were presented at the International

show abstract

Neurohypophyseal Hormone-Responsive Adenylate Cyclase from Mammalian Kidney

Cited by 47 publications

References 22 publications

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

Effect of Antidiuretic Hormone Upon Urinary Concentrating Ability and Medullary c-AMP Formation in Neonatal Piglets

Effects of Colchicine and Vinblastine on the Cellular Action of Vasopressin in Mammalian Kidney A POSSIBLE ROLE OF MICROTUBULES

The Handling of Immunoreactive Vasopressin by the Isolated Perfused Rat Kidney

Contact Info

Product

Resources

About