The Handling of Immunoreactive Vasopressin by the Isolated Perfused Rat Kidney

Rabkin, Ralph; Share, Leonard; Payne, Paul A.; Young, Judy; Crofton, J. T.

doi:10.1172/jci109279

Cited by 28 publications

(8 citation statements)

References 29 publications

(22 reference statements)

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“…The same approach to our data would lead to a smaller value (approximately 8 min from the data shown on Fig. It is also in agreement with earlier [20] and more recent [5] work using the isolated perfused rabbit [20] or rat [5] kidney. However, the two half-lives derived by Wilson et af.…”

Section: Discussionsupporting

confidence: 91%

Metabolic clearance rate of immunoreactive vasopressin in man

Benmansour

Rainfray

Paillard

et al. 1982

Eur J Clin Investigation

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Metabolic clearance of synthetic arginine vasopressin (AVP) has been measured in sixteen healthy subjects and ten uraemic patients on maintenance haemodialysis. Plasma AVP was measured using a specific radioimmunoassay at different intervals after a single injection of 2 micrograms AVP. The theoretical curve which fitted best with the disappearance curve was the sum of two exponentials in twenty-two subjects and of three exponentials in the other four. Metabolic clearance rate and the volume of fast initial distribution were 287.1 ml min-1 (m2)-1 and 219.3 ml/kg b.w., respectively, in normal subjects. Metabolic clearance rate was considerably lower in the uraemic group. This emphasizes the role of kidneys in the degradation of AVP and may account, at least in part, for the higher basal plasma value of this hormone observed in uraemic patients.

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Section: Discussionsupporting

confidence: 91%

Metabolic clearance rate of immunoreactive vasopressin in man

Benmansour

Rainfray

Paillard

et al. 1982

Eur J Clin Investigation

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“…This study, utilizing the IPRK, demonstrated that renal CGRP clearance occurs in the filtering kidney but almost ceases with the abolition of filtration. This finding is consistent with the elimination pattern of many other peptides, including parathyroid hormone, glucagon, growth hormone and prolactin (but not insulin or arginine vasopressin) where renal elimination is by intraparenchymal degradation after tubular reabsorption, with little or no active degradation in the glomerular capillary endothelium (Maack 1974;Rabkin & Kitaji 1978, 1983Rabkin et al 1979;Carone & Peterson 1980). Only 25% of total CGRP clearance can be accounted for by CGRP clearance from organ sites, including lung, liver, kidney, gut and brain (Brash et al 1988).…”

Section: Discussion Referencessupporting

confidence: 80%

The Intrarenal Site of Calcitonin Gene‐related Peptide Degradation in the Isolated Perfused Rat Kidney

Rubinstein

Fletcher

Shulkes

1993

Clin Exp Pharma Physio

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1. Calcitonin gene-related peptide (CGRP) is a potent vaso-active 37 amino acid peptide, typically elevated in plasma from patients with medullary thyroid cancer (MTC), but undetectable in the plasma of normal subjects. 2. The kidney is a major site for the clearance of exogenously infused CGRP but the intrarenal site of this clearance is unknown. Extra-organ clearance is also significant for CGRP, and whereas the site and mechanism of this degradation remain uncertain, the vasculature has been postulated as the most likely site. 3. The isolated perfused rat kidney (IPRK) was studied to (i) localize the intrarenal site of CGRP clearance and (ii) determine the contribution of the renal vasculature to the clearance of CGRP. The half-life of CGRP in the filtering IPRK was 63.9 +/- 4.5 min, whereas blocking of filtration by elevation of the perfusate osmolarity abolished the degradation. This suggests that (i) renal CGRP degradation occurs after glomerular filtration with intratubular metabolism and (ii) that there is no active CGRP degradation in the (glomerular) capillary endothelium. 4. These results do not support the theory that renal vascular endothelium plays a major active role in CGRP degradation.

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“…12 Maintenance on water ad libitum for 24 hours did not change AVP levels, whereas infusion of normal saline increased them by over fourfold. There are two possible explanations for the increased AVP seen in the subtotally-nephrectomized saline-infused animals.…”

Section: Discussionmentioning

confidence: 91%

Vasopressin in salt-induced hypertension of experimental renal insufficiency.

DiPette

Gavras²,

North³

et al. 1982

Hypertension

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SUMMARY The purpose of these experiments was to determine if arginine vasopressin (AVP) contributes to the blood pressure elevation induced by salt excess in renal insufficiency. Conscious rats were studied 6 days after surgical removal of 85% renal mass, which resulted hi renal Insufficiency as evidenced by an elevation in BUN and creatlnine. Four groups of subtotally nephrectomized animals were studied; two at time zero and two following a 24-hour 0.9% NaCI Intravenous (l.v.) infusion. One group at each time had plasma AVP determined, while the other was given 30 ^g I.T. of a specific Inhibitor of the pressor effect of AVP. A fifth subtotally nephrectomized and a sixth normal nonsurgical group were observed without infusion for 24 hours, at which time plasma AVP was determined. Subtotal nephrectomy alone increased baseline BP and tended to increase plasma AVP levels over those of normal control animals. Twenty-four hour saline infusion increased BP in both infused groups by 29 ± 4 and 30 ± 3 mm Hg respectively. No change in BP occurred in either the subtotally nephrectomized or normal animals observed without infusion for 24 hours. Following saline infusion, BP decreased by 20.2 ± 1.5 in response to the AVP inhibitor as compared to 13 ± 1.5 mm Hg at time zero (p < 0.001). AVP was 42 ± 9.6 following saline infusion as compared to 10.5 ± 1.5 pg/ml at time zero (p < 0.001). Six of the saline-infused animals had urine collected over the 24 hours. The percent of Na excreted/Na infused was 83%, while the percent of volume excreted/volume infused was 91.5% (p < 0.05), demonstrating to an extent selective retention of Na as opposed to volume. Taken

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The Handling of Immunoreactive Vasopressin by the Isolated Perfused Rat Kidney

Cited by 28 publications

References 29 publications

Metabolic clearance rate of immunoreactive vasopressin in man

Metabolic clearance rate of immunoreactive vasopressin in man

The Intrarenal Site of Calcitonin Gene‐related Peptide Degradation in the Isolated Perfused Rat Kidney

Vasopressin in salt-induced hypertension of experimental renal insufficiency.

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