Neurogenic potential of human mesenchymal stem cells isolated from bone marrow, adipose tissue and endometrium: a Comparative study

Zemelko, V. I.; Kozhukharova, Irina; Алексеенко, Л. Л.; Домнина, А. П.; Reshetnikova, Galina; Puzanov, M. V.; Dmitrieva, Renata I.; Гринчук, Т. М.; Nikolsky, Nikolay; Anisimov, S. V.

doi:10.1134/s1990519x13030140

Cited by 34 publications

(31 citation statements)

References 41 publications

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“…In contrast, BMSCs can only be continuously cultured for 60 d, with up to 12 population doublings [10] . Another study reported that the population-doubling time in umbilical cord-derived mesenchymal stem cells (UCMSCs) was much lower than that of ADSCs, but UCMSCs showed earlier morphological changes and a more rapid decline in amplification ability [9][10][11]. Some studies found that amniotic fluid-derived MSCs and ADSCs exhibited different levels of telomerase activity [12] , suggesting different amplification characteristics.…”

Section: Introductionmentioning

confidence: 94%

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Comparative analysis of biological characteristics of adult mesenchymal stem cells with different tissue origins

Chen

Mou

et al. 2015

Asian Pacific Journal of Tropical Medicine

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Section: Introductionmentioning

confidence: 94%

“…Meng et al found that MenSCs expressed OCT-4, but not NANOG or SSEA-4 [5][6][7] , while Rossignoli et al [8] reported that 19.4% of MenSCs were SSEA-4-positive by flow cytometry. Zemel'ko et al [9] showed high expression of SSEA-4 by immunofluorescence.…”

Section: Introductionmentioning

confidence: 98%

Comparative analysis of biological characteristics of adult mesenchymal stem cells with different tissue origins

Chen

Mou

et al. 2015

Asian Pacific Journal of Tropical Medicine

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“…It has been already proposed that in ASD cell-based treatment, the positive effects that could be mediated by stem cells could be achieved through the trophic and immunomodulatory properties [9]. Implanted stem cells (whether autologous or donor) are able to affect the recipient immune system through two proposed mechanisms: (1) cell-to-cell contact activation mechanism, through which transplanted stem cells switch proinflammatory macrophages to anti-inflammatory macrophages [15, 16]; (2) paracrine-secretome activity [17]. It is noteworthy to consider that ASDs are associated with significant immune alterations and proinflammatory cytokines overproduction [18].…”

Section: Stem Cells In Autism Spectrum Disordersmentioning

confidence: 99%

Perspectives on the Use of Stem Cells for Autism Treatment

Siniscalco

Bradstreet²,

Sych³

et al. 2013

Stem Cells International

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Autism and autism spectrum disorders (ASDs) are complex neurodevelopmental disorders. ASDs are clinically defined by deficits in communication, social skills, and repetitive and/or restrictive interests and behaviours. With the prevalence rates for ASDs rapidly increasing, the need for effective therapies for autism is a priority for biomedical research. Currently available medications do not target the core symptoms, can have markedly adverse side-effects, and are mainly palliative for negative behaviours. The development of molecular and regenerative interventions is progressing rapidly, and medicine holds great expectations for stem cell therapies. Cells could be designed to target the observed molecular mechanisms of ASDs, that is, abnormal neurotransmitter regulation, activated microglia, mitochondrial dysfunction, blood-brain barrier disruptions, and chronic intestinal inflammation. Presently, the paracrine, secretome, and immunomodulatory effects of stem cells would appear to be the likely mechanisms of application for ASD therapeutics. This review will focus on the potential use of the various types of stem cells: embryonic, induced pluripotential, fetal, and adult stem cells as targets for ASD therapeutics.

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“…Most studies on neural differentiation of MSCs were carried out with human and rodent cells (2,(5)(6)(7)(8)(9)(10)(11)(12)(13). In veterinary medicine, dogs are interesting for development of novel regenerative treatments, and in addition to bene ting canine patients, these therapies might show translational potential as dogs could be very interesting model of human neurological disorders.…”

Section: Introductionmentioning

confidence: 99%

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells

Mihevc

Grgich

Kopitar

et al. 2020

Preprint

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Background: Ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine especially for replacing damaged neuronal tissue in different neurological disorders. Reprogramming of ASCs can be induced by supplying growth medium with chemical neurogenic inductors and/or specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement and retinoic acid. First the cells were preconditioned in proliferation medium, followed by induction of neuronal differentiation. Six canine ASCs cell lines were assessed, half from female and half from male donors. The cell morphology, growth dynamics, viability were observed along with expression of neuron and astrocyte specific markers, which were assessed by immunocytochemistry and flow cytometry. Results: After 3, 6 and 9 days, elongated neural-like cells with bipolar elongations were observed and some oval cells with light nuclei appeared. After three and nine days of neural induction, differentiation into neurons and glial cells was observed. Expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H) and glial fibrillary acidic protein (GFAP) was observed by immunocytochemistry. High GFAP expression (between 70 and 90% of all cells) was detected after three days of growth in neural induction medium a (NIMa) by flow cytometry, and expression of adult neuronal markers NF-H and microtubule associated protein-2 (MAP2) was detected in around 25% of cells. After nine days of ASCs differentiation a drop in expression rates of all markers was detected. There were no differences between neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium the canine ASCs were able to transform to neural lineages, bearing markers of neuronal and glial cells and also displayed the typical neuronal morphology. Differentiated ASCs can be a source of neural cellular lineages for regenerative therapy of nerve damage and also could be applicable for modeling of neurodegenerative diseases.

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Neurogenic potential of human mesenchymal stem cells isolated from bone marrow, adipose tissue and endometrium: a Comparative study

Cited by 34 publications

References 41 publications

Comparative analysis of biological characteristics of adult mesenchymal stem cells with different tissue origins

Comparative analysis of biological characteristics of adult mesenchymal stem cells with different tissue origins

Perspectives on the Use of Stem Cells for Autism Treatment

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells

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