Neurodevelopment vs. the immune system: Complementary contributions of maternally-inherited gene transcripts and proteins to successful embryonic development in fish

Żarski, Daniel; Cam, Aurélie Le; Fröhlich, Thomas; Kösters, Miwako; Klopp, Christophe; Nynca, Joanna; Ciesielski, Sławomir; Sarosiek, Beata; Dryl, Katarzyna; Montfort, Jérôme; Król, Jarosław; Fontaine, Pascal; Ciereszko, Andrzej; Bobe, Julien

doi:10.1016/j.ygeno.2021.09.003

Cited by 7 publications

(13 citation statements)

References 78 publications

(137 reference statements)

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“…Fast-forward to today's exciting era of high throughput sequencing enables large-scale proteomic profiling, which has opened up new perspectives in every field of biology. Proteomic approaches integrated with transcriptomics have been applied successfully to reveal the molecular mechanisms involved in reproduction and development in numerous animals [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Integrated Proteomic and Transcriptomic Analysis of Gonads Reveal Disruption of Germ Cell Proliferation and Division, and Energy Storage in Glycogen in Sterile Triploid Pacific Oysters (Crassostrea gigas)

Chen

2021

Cells

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Triploid oysters have poor gonadal development, which can not only bring higher economic benefits but also have a potential application in the genetic containment of aquaculture. However, the key factors that influence germ cell development in triploid oysters remain unclear. In this study, data-independent acquisition coupled to transcriptomics was applied to identify genes/proteins related to sterility in triploid Crassostrea gigas. Eighty-four genes were differentially expressed at both the protein and mRNA levels between fertile and sterile females. For male oysters, 207 genes were differentially expressed in the transcriptomic and proteomic analysis. A large proportion of downregulated genes were related to cell division, which may hinder germ cell proliferation and cause apoptosis. In sterile triploid females, a primary cause of sterility may be downregulation in the expression levels of certain mitotic cell cycle-related genes. In sterile triploid males, downregulation of genes related to cell cycle and sperm motility indicated that the disruption of mitosis or meiosis and flagella defects may be linked with the blocking of spermatogenesis. Additionally, the genes upregulated in sterile oysters were mainly associated with the biosynthesis of glycogen and fat, suggesting that sterility in triploids stimulates the synthesis of glycogen and energy conservation in gonad tissue.

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Section: Introductionmentioning

confidence: 99%

Integrated Proteomic and Transcriptomic Analysis of Gonads Reveal Disruption of Germ Cell Proliferation and Division, and Energy Storage in Glycogen in Sterile Triploid Pacific Oysters (Crassostrea gigas)

Chen

2021

Cells

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“…As the larvae grow, their nervous system rapidly develops to facilitate their interaction with external stimuli and control their motor skills and overall behavioral abilities. Moreover, it has already been highlighted that neurogenesis events in the offspring can be affected by maternally inherited mRNA [20, 44], and this has also been reflected in the personality/behavior of the fish [18]. WGCNA showed that the genes involved in nervous system development were correlated with the length of larvae at hatching.…”

Section: Discussionmentioning

confidence: 99%

“…This process is crucial for enhancing sensory perception, motor coordination, and cognitive functions in larvae (Nelson and Granato, 2022). Previous studies discussed the nervous systems’ development could potentially be pre-programmed by the molecular content inherited maternally (Żarski et al, 2021, 2020b). This, along with environmental factors may influence the trajectory of nervous system development and impact future behaviour and adaptive responses (Colson et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…After amplification, a melting curve was made to check the amplification specificity. Relative expression was normalized using the geometric mean of expression values recorded for four reference genes, namely, acyl-CoA dehydrogenase long chain (acadl), thioredoxin 2 (txn2), glutathione s-transferase alpha 1 (gsta1), and wd repeat domain 83 opposite strand (wdr83os) , exhibiting the most stable expression level (revealed based on the transcriptomic data obtained) [20]. Finally, the data between the groups were analyzed with the t test (GraphPad Prism 9), and significant differences were considered at p □<□0.05.…”

Section: Methodsmentioning

confidence: 99%

“…Gene Ontology (GO) analysis GO analysis was performed by following the approach described by Żarski et al [20].…”

Section: Differential Expression Analysismentioning

confidence: 99%

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Does transcriptome of freshly hatched fish larvae describe past or predict future developmental trajectory?

Debernardis,

Palińska-Żarska,

Judycka

et al. 2023

Preprint

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Background: The larval stage plays a crucial role in the ontogeny of many fish species, leading to profound changes in morphology, physiology and behavior. How larvae face metamorphoses can be associated with their quality, which can vary significantly among the same family or even cohort. This variability depends on the interaction of genetic and nongenetic factors, which are key phenotypic determinants that further affect the overall survival of wild fish and adaptability of cultured stocks. Although researchers and aquaculturists often refer to larvae quality as an important parameter discriminating high- and low-performing stocks, there is currently no universally accepted definition or critically verified set of quality indicators. Therefore, in this study, we generated unique families of Eurasian perch (Perca fluviatilis) larvae to follow their performance during the larval period. Next, we integrated the zootechnical performance data with transcriptomics data from freshly hatched larvae to identify specific molecular quality markers. Results: Zootechnical data revealed statistically significant differences (p < 0.05) in performance between 16 families reared. The RNA-seq data were processed using weighted gene correlation network analysis (WGCNA) and showed particularly significant interactions between gene modules and the length of larvae at the mouth-opening stage, emerging as an important quality-related trait. Next, by comparing the transcriptomic profiles of larvae with high and low lengths at mouth opening, we identified several differentially expressed genes, here chosen as putative larvae quality markers, involved in metabolic processes and antioxidant responses, such as fatty acid desaturase 2 (fads2) and glutathione peroxidase 1b (gpx1b). Conclusions: The findings highlight the length of the larvae at hatching as a valid parameter of larvae quality. Initial length is not just a numerical measurement; it serves as a specific indicator that strongly influences future larval development and survival. 'Longer' larvae appear to benefit from a higher metabolic rate, enhanced antioxidant defenses and a more advanced nervous system. As a result of these insights, we propose genes such as fads2, ctsl1, and gpx1b as potential biomarkers for evaluating larval quality. Our study represents a significant step toward a clear understanding of the factors that determine the quality of fish larvae, with implications for improving aquaculture practices.

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Comparative proteomic analysis reveals the importance of the protective role of ovarian fluid over eggs during the reproduction of pikeperch

et al. 2022

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Neurodevelopment vs. the immune system: Complementary contributions of maternally-inherited gene transcripts and proteins to successful embryonic development in fish

Cited by 7 publications

References 78 publications

Integrated Proteomic and Transcriptomic Analysis of Gonads Reveal Disruption of Germ Cell Proliferation and Division, and Energy Storage in Glycogen in Sterile Triploid Pacific Oysters (Crassostrea gigas)

Integrated Proteomic and Transcriptomic Analysis of Gonads Reveal Disruption of Germ Cell Proliferation and Division, and Energy Storage in Glycogen in Sterile Triploid Pacific Oysters (Crassostrea gigas)

Does transcriptome of freshly hatched fish larvae describe past or predict future developmental trajectory?

Comparative proteomic analysis reveals the importance of the protective role of ovarian fluid over eggs during the reproduction of pikeperch

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