Comparative proteomic analysis reveals the importance of the protective role of ovarian fluid over eggs during the reproduction of pikeperch

Nynca, Joanna; Żarski, Daniel; Fröhlich, Thomas; Köster, Maria; Bobe, Julien; Ciereszko, Andrzej

doi:10.1016/j.aquaculture.2021.737656

Cited by 6 publications

(12 citation statements)

References 77 publications

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“…Our analysis therefore assumes similar gene functions in lacertids as in humans, which is a clear simplification. However, since only about 1% of protein sequences in the UniProt database have experimentally verified functions, the transfer of protein annotations between different species is a widespread approach [ 44 , 45 , 46 , 47 ]. Nonetheless, our results should be taken as a first approximation of the potential functions of FG proteins in sand lizards, and are in need of validation in future studies.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, the DAVID bioinformatics resource [ 59 , 60 ] was applied, similarly using human taxonomy (506 recognized genes). The transfer of functional annotations between species is commonplace in proteomic and genomic studies [ 44 , 45 , 46 , 47 ]. Although the use of human reference databases (or other well-annotated databases) may increase the number of annotations, extrapolation of protein function to other organisms should be cautiously interpreted.…”

Section: Methodsmentioning

confidence: 99%

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Functional Protein Composition in Femoral Glands of Sand Lizards (Lacerta agilis)

et al. 2022

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Proteins are ubiquitous macromolecules that display a vast repertoire of chemical and enzymatic functions, making them suitable candidates for chemosignals, used in intraspecific communication. Proteins are present in the skin gland secretions of vertebrates but their identity, and especially, their functions, remain largely unknown. Many lizard species possess femoral glands, i.e., epidermal organs primarily involved in the production and secretion of chemosignals, playing a pivotal role in mate choice and intrasexual communication. The lipophilic fraction of femoral glands has been well studied in lizards. In contrast, proteins have been the focus of only a handful of investigations. Here, we identify and describe inter-individual expression patterns and the functionality of proteins present in femoral glands of male sand lizards (Lacerta agilis) by applying mass spectrometry-based proteomics. Our results show that the total number of proteins varied substantially among individuals. None of the identified femoral gland proteins could be directly linked to chemical communication in lizards, although this result hinges on protein annotation in databases in which squamate semiochemicals are poorly represented. In contrast to our expectations, the proteins consistently expressed across individuals were related to the immune system, antioxidant activity and lipid metabolism as their main functions, showing that proteins in reptilian epidermal glands may have other functions besides chemical communication. Interestingly, we found expression of the Major Histocompatibility Complex (MHC) among the multiple and diverse biological processes enriched in FGs, tentatively supporting a previous hypothesis that MHC was coopted for semiochemical function in sand lizards, specifically in mate recognition. Our study shows that mass spectrometry-based proteomics are a powerful tool for characterizing and deciphering the role of proteins secreted by skin glands in non-model vertebrates.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Functional Protein Composition in Femoral Glands of Sand Lizards (Lacerta agilis)

et al. 2022

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“…Despite the importance of sturgeon oocytes for aquaculture, little information is available on their protein composition. Proteomic techniques are powerful tools for studying proteins on a large scale and have been successfully used to identify egg proteins in various fish species, such as pikeperch (1296 proteins) [ 8 ] and zebrafish (2535 proteins) [ 9 ]. Similarly, OF proteins have been identified in chinook salmon (174 proteins) [ 10 ], pikeperch (796 proteins) [ 8 ] and rainbow trout (54 proteins) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Proteomic techniques are powerful tools for studying proteins on a large scale and have been successfully used to identify egg proteins in various fish species, such as pikeperch (1296 proteins) [ 8 ] and zebrafish (2535 proteins) [ 9 ]. Similarly, OF proteins have been identified in chinook salmon (174 proteins) [ 10 ], pikeperch (796 proteins) [ 8 ] and rainbow trout (54 proteins) [ 11 ]. However, only a limited number of proteins have been identified in the OF and eggs of sturgeon species, such as sterlets and Persian sturgeon, and protein counts ranged from 30 to 80 [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…A comprehensive approach would elucidate the relationship between the OF and eggs. Only one recent proteomic study on pikeperch (a teleost fish) investigated OF and eggs and addressed this issue [ 8 ]. In-depth proteomic studies of eggs successfully identified potential biomarkers for egg quality in pikeperch [ 8 ] and zebrafish [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Kodzik,

Ciereszko,

Szczepkowska

et al. 2024

BMC Genomics

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Background Sturgeon species are living fossils that exhibit unique reproductive characteristics, and elucidation of the molecular processes governing the formation and quality of sturgeon eggs is crucial. However, comprehensive data on the protein composition of sturgeon ovarian fluid (OF) and eggs and their functional significance are lacking. To address this knowledge gap, the aim of the present study was to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon OF and eggs using liquid chromatography–mass spectrometry (LC–MS/MS). Results A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components. Conclusions This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.

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In vitro maturation of African catfish (Clarias gariepinus, Burchell, 1822) oocytes results in viable larvae

Kitanović,

Marinović,

Quyến

et al. 2024

Aquaculture

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Comparative proteomic analysis reveals the importance of the protective role of ovarian fluid over eggs during the reproduction of pikeperch

Cited by 6 publications

References 77 publications

Functional Protein Composition in Femoral Glands of Sand Lizards (Lacerta agilis)

Functional Protein Composition in Femoral Glands of Sand Lizards (Lacerta agilis)

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

In vitro maturation of African catfish (Clarias gariepinus, Burchell, 1822) oocytes results in viable larvae

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