2012
DOI: 10.1007/s12031-012-9855-9
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Neurochemical Characterization of Zinc Transporter 3-Like Immunoreactive (ZnT3+) Neurons in the Intramural Ganglia of the Porcine Duodenum

Abstract: The SLC30 family of divalent cation transporters is thought to be involved in the transport of zinc in a variety of cellular pathways. Zinc transporter 3 (ZnT3) is involved in the transport of zinc into synaptic vesicles or intracellular organelles. As the presence of ZnT3 immunoreactive neurons has recently been reported in both the central and peripheral nervous systems of the rat, the present study was aimed at disclosing the presence of a zinc-enriched neuron enteric population in the porcine duodenum to e… Show more

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Cited by 11 publications
(22 citation statements)
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“…The obtained results are in agreement with previous studies, where numerous ZnT3-positive neurons have been described in other fragments of porcine digestive tract (Wojtkiewicz et al 2012a, b). Nonetheless, exact functions of ZnT3 in the enteric nervous system remain unknown.…”
Section: Discussionsupporting
confidence: 93%
“…The obtained results are in agreement with previous studies, where numerous ZnT3-positive neurons have been described in other fragments of porcine digestive tract (Wojtkiewicz et al 2012a, b). Nonetheless, exact functions of ZnT3 in the enteric nervous system remain unknown.…”
Section: Discussionsupporting
confidence: 93%
“…In the present investigation, CART-like immunoreactive neurons was observed in the MP of the porcine transverse colon, what is in accordance with previous studies, where expression of CART was noted in different parts of the GI tract in various species [13,28]. The presence of CART in the nerve structures populations studied were observed.…”
Section: Discussionsupporting
confidence: 93%
“…After at least two weeks, the fragments of liver were frozen at −23 °C and cut into 10 μm-thick sections using a microtome (Microm, HM 525, Walldorf, Germany). The sections were subjected to a routine double-labeling immunofluorescence technique according to the method described previously by Gonkowski [ 38 , 40 ] and Wojtkiewicz [ 74 , 75 ]. A condensed description of the method is as follows: 45 min of drying; incubation with a blocking solution, which included 10% normal goat serum, 0.1% bovine serum albumin, 0.01% NaN 3 , Triton X−100, and thimerozal in phosphate buffered saline (PBS) for 1 h; overnight incubation with a mixture of two “primary” antibodies raised in different species and directed towards vesicular acetylcholine transporter (VAChT), and one of the aforementioned substances i.e., CART, substance P, CGRP, GAL, or PACAP; incubation (for 1 h) with species-specific antisera conjugated to Fluorescein (FITC) or biotin, which was visualized by a streptavidin-CY3 complex (the specification of intravenous primary and secondary antibodies used in the present study is shown in Table 2 ).…”
Section: Methodsmentioning
confidence: 99%