Neurobiological effects of repeated radiofrequency exposures in male senescent rats

Bouji, Marc; Lecomte, Anthony; Gamez, Christelle; Blazy, Kelly; Villegier, Anne‐Sophie

doi:10.1007/s10522-016-9654-8

Cited by 16 publications

(16 citation statements)

References 73 publications

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“…In addition, a previous study detected no changes in the Iba-1 expression of microglia in mouse brains after acute or chronic exposure to RF-EMFs based on Iba-1 immunostaining [ 26 ]. Consistent with the previous studies showing negligible effects on GFAP and Iba-1 expression levels [ 26 , 41 ], the present study found no differences in the protein levels of GFAP and Iba-1. Thus, these data suggest that 8 months of RF-EMF exposure did not affect age-related increases in astrocyte and microglia marker expression in the mouse brain.…”

Section: Discussionsupporting

confidence: 93%

“…However, no changes in the levels of GFAP were observed after a single exposure to 1800 MHz signals [ 24 ]. GFAP immunostaining in the cortex and hippocampus showed no changes after the heads of both adult and aged rats were exposed to RF-EMF [ 41 ]. In addition, a previous study detected no changes in the Iba-1 expression of microglia in mouse brains after acute or chronic exposure to RF-EMFs based on Iba-1 immunostaining [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice

Jeong

Son

Han

et al. 2018

IJMS

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The expansion of mobile phone use has raised questions regarding the possible biological effects of radiofrequency electromagnetic field (RF-EMF) exposure on oxidative stress and brain inflammation. Despite accumulative exposure of humans to radiofrequency electromagnetic fields (RF-EMFs) from mobile phones, their long-term effects on oxidative stress and neuroinflammation in the aging brain have not been studied. In the present study, middle-aged C57BL/6 mice (aged 14 months) were exposed to 1950 MHz electromagnetic fields for 8 months (specific absorption rate (SAR) 5 W/kg, 2 h/day, 5 d/week). Compared with those in the young group, levels of protein (3-nitro-tyrosine) and lipid (4-hydroxy-2-nonenal) oxidative damage markers were significantly increased in the brains of aged mice. In addition, levels of markers for DNA damage (8-hydroxy-2′-deoxyguanosine, p53, p21, γH2AX, and Bax), apoptosis (cleaved caspase-3 and cleaved poly(ADP-ribose) polymerase 1 (PARP-1)), astrocyte (GFAP), and microglia (Iba-1) were significantly elevated in the brains of aged mice. However, long-term RF-EMF exposure did not change the levels of oxidative stress, DNA damage, apoptosis, astrocyte, or microglia markers in the aged mouse brains. Moreover, long-term RF-EMF exposure did not alter locomotor activity in aged mice. Therefore, these findings indicate that long-term exposure to RF-EMF did not influence age-induced oxidative stress or neuroinflammation in C57BL/6 mice.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice

Jeong

Son

Han

et al. 2018

IJMS

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“…Regarding the effects of RF exposure on anxiety-like behavior, the results remain controversial. Bouji reported that exposure to RF field (900 MHz, SAR 6 W/kg, 45 min/day for 1 month) decreased anxiety-related behaviors in both young and aged rats [ 11 ]. Kumlin found that exposure to RF field (900 MHz, SAR 9W/kg) for 2 h/day, 5 days per week (exposure from the age of 21 days to the age of 8 weeks) did not increase the level of anxiety in rats [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…In terms of the effects of RF field on brain function, Wang found that although single exposure to 1800 MHz RF field for 30 min with specific absorption rates (SARs) in brain at 2.42, 2.86 and 3.3 W/kg did not affect spontaneous locomotor activity, it increased recognition memory in mice [ 10 ]. In addition, Bouji reported that after exposing the heads of the animals to 900 MHz RF field for 1 month (SAR 6 W/kg, 45 min/day), the spatial memory, emotional memory and locomotor activity did not change, but the anxiety-related behavior decreased in male senescent rats [ 11 ]. Arendash reported that exposure to 918 MHz RF field (SAR: 0.25 W/kg, 1 h/day for 4–7 months) could protect against and reverse cognitive impairment in Alzheimer’s disease mice [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of 1.8 GHz Radiofrequency Fields on the Emotional Behavior and Spatial Memory of Adolescent Mice

Zhang

Guo

et al. 2017

IJERPH

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The increasing use of mobile phones by teenagers has raised concern about the cognitive effects of radiofrequency (RF) fields. In this study, we investigated the effects of 4-week exposure to a 1.8 GHz RF field on the emotional behavior and spatial memory of adolescent male mice. Anxiety-like behavior was evaluated by open field test (OFT) and elevated plus maze (EPM) test, while depression-like behavior was evaluated by sucrose preference test (SPT), tail suspension test (TST) and forced swim test (FST). The spatial learning and memory ability were evaluated by Morris water maze (MWM) experiments. The levels of amino acid neurotransmitters were determined by liquid chromatography-mass spectrometry (LC-MS). The histology of the brain was examined by hematoxylin-eosin (HE) staining. It was found that the depression-like behavior, spatial memory ability and histology of the brain did not change obviously after RF exposure. However, the anxiety-like behavior increased in mice, while, the levels of γ-aminobutyric acid (GABA) and aspartic acid (Asp) in cortex and hippocampus significantly decreased after RF exposure. These data suggested that RF exposure under these conditions do not affect the depression-like behavior, spatial memory and brain histology in adolescent male mice, but it may however increase the level of anxiety, and GABA and Asp were probably involved in this effect.

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“…We cannot exclude that the repetition of the whole protocol (NMDA i.t. administrations, restraint, heat nociceptive stimulation and/or RF-EMF exposures) may have influenced data variability through possible side effects [ 17 , 18 , 22 , 23 , 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Radiofrequency Electromagnetic Fields on Thermal Sensitivity in the Rat

Ouadah

Blazy

Villegier

2020

IJERPH

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The World Health Organization and the French Health Safety Agency (ANSES) recognize that the expressed pain and suffering of electromagnetic field hypersensitivity syndrome (EHS) people are a lived reality requiring daily life adaptations to cope. Mechanisms involving glutamatergic N-methyl d-aspartate (NMDA) receptors were not explored yet, despite their possible role in hypersensitivity to chemicals. Here, we hypothesized that radiofrequency electromagnetic field (RF-EMF) exposures may affect pain perception under a modulatory role played by the NMDA receptor. The rats were exposed to RF-EMF for four weeks (five times a week, at 0 (sham), 1.5 or 6 W/kg in restraint) or were cage controls (CC). Once a week, they received an NMDA or saline injection before being scored for their preference between two plates in the two-temperatures choice test: 50 °C (thermal nociception) versus 28 °C. Results in the CC and the sham rats indicated that latency to escape from heat was significantly reduced by −45% after NMDA, compared to saline treatment. Heat avoidance was significantly increased by +40% in the 6 W/kg, compared to the sham exposed groups. RF-EMF effect was abolished after NMDA treatment. In conclusion, heat avoidance was higher after high brain-averaged specific absorption rate, affording further support for possible effect of RF-EMF on pain perception. Further studies need to be performed to confirm these data.

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Neurobiological effects of repeated radiofrequency exposures in male senescent rats

Cited by 16 publications

References 73 publications

Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice

Impact of Long-Term RF-EMF on Oxidative Stress and Neuroinflammation in Aging Brains of C57BL/6 Mice

Effects of 1.8 GHz Radiofrequency Fields on the Emotional Behavior and Spatial Memory of Adolescent Mice

Effect of Radiofrequency Electromagnetic Fields on Thermal Sensitivity in the Rat

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