Neuro-electronic interfacing with multielectrode arrays

Rutten, Wim; Smit, J.P.A.; Frieswijk, T.A.; Bielen, J.A.; Brouwer, A.L.H.; Buitenweg, Jan R.; Heida, Tjitske

doi:10.1109/51.765188

Cited by 34 publications

(23 citation statements)

References 22 publications

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“…Another method for single-cell recording in which the distance of the membrane to a planar surface plays a crucial role is the use of arrays of planar micro-electrodes (microelectrode arrays (MEAs)) for parallel recording of extracellular potentials in close vicinity of adherent cells (see review by POTTER (2001) transmission from cell to electrode can be described with an equivalent electrical circuit (BOVE et al, 1997;BUITENWEG et al, 1998;FROMHERZ, 1999;FROMHERZ and STETT, 1995;MAHER et al, 1999;RUTTEN et al, 1999;WEIS et aL, 1996). in these models, a sealing resistance represents the electrolytic cleft in the contact, as in the planar patch approach described above.…”

Section: Loose-seal Recording With Planar Electrodesmentioning

confidence: 99%

Patch-clamping of primary cardiac cells with micro-openings in polyimide films

Stett

Bucher

Burkhardt

et al. 2003

Med. Biol. Eng. Comput.

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Patch-clamping is a powerful method for investigating the function and regulation of ionic channels. Currently, great efforts are being made to automate this method. As a step towards this goal, the feasibility of patch-clamping primary cells with a microscopic opening in a planar substrate was tested. Using standard microfabrication and ion beam technology, small-diameter openings (2 and 4 microm) were formed in polyimide films (thickness 6.5 microm). Single cells (sheep Purkinje heart cells, Chinese hamster ovary cells) in a suspension were positioned on top of the opening and sucked towards the opening to improve adhesion of the cell to the planar substrate, hence increasing the seal resistance. Voltage/current measurements yielded a median seal resistance of 1.3 Mohms with 4 microm openings (n=24) and 26.0 Mohms with 2 microm openings (n = 75), respectively. With 2 microm openings, successful loose-patch recordings of TTX-sensitive inward currents and action potentials in sheep Purkinje heart cells (n = 18) were made. In rare cases, gigaseals (n = 4) were also measured, and a whole-cell configuration (n = 1) could be established. It was concluded that the simple planar patch approach is suitable for automated loose-patch recordings from cells in suspension but will hardly be suitable for high-throughput whole-cell patch-clamping with high-resistance seals.

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Section: Loose-seal Recording With Planar Electrodesmentioning

confidence: 99%

Patch-clamping of primary cardiac cells with micro-openings in polyimide films

Stett

Bucher

Burkhardt

et al. 2003

Med. Biol. Eng. Comput.

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“…With the advancement of microfabrication techniques, various microelectrode arrays (MEAs), including linear array, 2D planar array or 3D needle array, have been designed as cell culture devices in vitro (Rutten et al 1999). Investigations on the properties and mechanisms of excitable cells on MEA have led to various applications, including fundamental electrophysiological studies (Stenger et al 2001), pharmaceutical drug screening (Durick and Negulescu 2001), environmental toxic detection (Chiappalone et al 2003), and artificial neuronal prosthesis (Yagi et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterization of surface modification on microelectrode arrays for in vitro cell culture

Lin¹,

Chen²,

Liao³

et al. 2007

Biomed Microdevices

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This study aims to investigate surface-modified microelectrodes on the microelectrode arrays (MEAs) for neuronal interfaces with in vitro cell culture. The polyimide (PI) MEA was fabricated by using micro-electro-mechanical systems (MEMS) techniques. Self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) were utilized to modify the microelectrode surface of the MEA. The SAMs' modified surface of microelectrodes offered a reliable interface to immobilize biological ligands through covalent bonding. To increase biocompatibility, the poly-D-lysine (PDL) was immobilized on the SAMs' modified microelectrodes. Several analytical techniques were used to define the physical structure and functional groups of surface-modified gold microelectrodes on the MEA. Spectra of the Fourier transform infrared reflection (FTIR) were applied to characterize the molecular structure of MUA-SAMs and PDL on the microelectrodes. The spectra, two peaks of amide I (at 1,613 cm(-1)) and amide II (at 1,548 cm(-1)), revealed that covalent amide bonding existed in PDL-MUA-SAMs modified surfaces. The thickness and formation of the MUA and PDL were also observed and quantified by using an atomic force microscope (AFM). The impedance measurement of PDL-MUA-SAMs modified MEA only increased slightly to an average of 524.6 +/- 55.8 kOmega from 352.9 +/- 34.4 kOmega of bare gold microelectrode (p < 0.05, N = 20). In addition, the time-course changes of total impedance resulting from cell sealing resistance and gap reactance were recorded for 7 days for inferring the growth of cell lines on the electrode contact of modified MEA. The experiment of 3T3 fibroblasts, PC12 cells, primary glial cells, and primary cortical neurons cultured on the modified MEAs displayed a good adhesion rate. These biocompatibility assays demonstrated that the neuronal cells are able to grow in a proximity to PDL-MUA-SAMs modified microelectrodes of the MEAs for effective electrophysiological stimulation/sensing schemes and for future implantation purposes.

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“…CURRENT microelectrode technologies that record from a population of neurons simultaneously using arrays of multichannel microelectrodes enable us to understand the emergent properties of assemblies of neurons [1]- [6]. These are especially useful when combined with simultaneous measures of behavioral performance as pretrained animals perform sensory-perceptual tasks.…”

Section: Introductionmentioning

confidence: 99%

Electrostatic Microactuators for Precise Positioning of Neural Microelectrodes

Muthuswamy

Okandan

Jain

et al. 2005

IEEE Trans. Biomed. Eng.

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Microelectrode arrays used for monitoring single and multineuronal action potentials often fail to record from the same population of neurons over a period of time likely due to micromotion of neurons away from the microelectrode, gliosis around the recording site and also brain movement due to behavior. We report here novel electrostatic microactuated microelectrodes that will enable precise repositioning of the microelectrodes within the brain tissue. Electrostatic comb-drive microactuators and associated microelectrodes are fabricated using the SUMMiT V™ (Sandia's Ultraplanar Multilevel MEMS Technology) process, a five-layer polysilicon micromachining technology of the Sandia National labs, NM. The microfabricated microactuators enable precise bidirectional positioning of the microelectrodes in the brain with accuracy in the order of 1 μm. The microactuators allow for a linear translation of the microelectrodes of up to 5 mm in either direction making it suitable for positioning microelectrodes in deep structures of a rodent brain. The overall translation was reduced to approximately 2 mm after insulation of the microelectrodes with epoxy for monitoring multiunit activity. The microactuators are capable of driving the microelectrodes in the brain tissue with forces in the order of several micro-Newtons. Single unit recordings were obtained from the somatosensory cortex of adult rats in acute experiments demonstrating the feasibility of this technology. Further optimization of the insulation, packaging and interconnect issues will be necessary before this technology can be validated in long-term experiments.

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Neuro-electronic interfacing with multielectrode arrays

Cited by 34 publications

References 22 publications

Patch-clamping of primary cardiac cells with micro-openings in polyimide films

Patch-clamping of primary cardiac cells with micro-openings in polyimide films

Characterization of surface modification on microelectrode arrays for in vitro cell culture

Electrostatic Microactuators for Precise Positioning of Neural Microelectrodes

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