Neurite Outgrowth Is Enhanced by Anti-Idiotypic Monoclonal Antibodies to the Ganglioside GM1

Riggott, Marcia J.; Matthew, William Diller

doi:10.1006/exnr.1997.6459

Cited by 5 publications

(4 citation statements)

References 60 publications

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“…58 Anti‐idiotypic ganglioside monoclonal antibodies that were generated to mimic the biological properties of GM1 were shown to promote neurite outgrowth from central and peripheral nervous system neurons. 59,60 Western blotting with this antibody revealed four potential GM1‐binding proteins of molecular weights 93, 66, 57, and 45 kDa. The neurite‐promoting effects of extracellular matrix proteins such as laminin 61 have been attributed to their interaction with neuronal surface gangliosides.…”

Section: Overviewmentioning

confidence: 99%

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a

Ledeen

et al. 1998

Annals of the New York Academy of Sciences

115

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The pronounced increases in gangliosides belonging to the gangliotetraose family during the neurite outgrowth phase of neuronal differentiation have suggested a functional requirement for these substances related to process extension, arborization, and possibly synaptogenesis. Support for this hypothesis has come from a variety of experimental paradigms utilizing neuroblastoma cell lines, primary neuronal cultures, and observations on the developing nervous system. We have recently observed that differentiation of both primary neurons and neuroblastoma cells by Ca(2+)-elevating stimulants is characterized by upregulation of GM1 in the nuclear membrane. Immunostaining revealed these Ca(2+)-induced neurites to have axonal characteristics. Recent work has indicated that nuclear GM1 facilitates efflux of nuclear Ca2+, thereby contributing to the reduced level of nuclear Ca2+ that characterizes the differentiated neuron. Thus, while GM1 is generally recognized as a pluripotent molecule with several modulatory roles in the plasma membrane of developing and mature neurons, regulation of Ca2+ flux across the nuclear membrane is proposed as another critical function of this ganglioside in neuronal development, with special relevance to axonogenesis.

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Section: Overviewmentioning

confidence: 99%

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a

Ledeen

et al. 1998

Annals of the New York Academy of Sciences

115

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“…Resident microglia or immune cells may be induced to release cytokines and growth factors that (1) recruit oligodendrocyte progenitors to the site of damage, (2) increase oligodendrocyte progenitor proliferation, or (3) alter the lesion microenvironment to support remyelination Antibodies that bind to neurons also directly induce signals and alter cell morphology. mAbs against ganglioside GM1 suppress neurite outgrowth in vitro and in vivo [146,147], whereas anti-idiotypic antibodies to GM1 induce neurite extension [124]. mAbs to the ganglioside GD3 (R24) or to the cerebellar granule cell surface protein (TAG-1) induce activation of the Src family kinase Lyn and result in similar alteration in protein tyrosine phosphorylation.…”

Section: Mechanisms Of Antibody-mediated Cns Repairmentioning

confidence: 99%

Remyelination-Promoting Human IgMs: Developing a Therapeutic Reagent for Demyelinating Disease

Warrington

Rodriguez²

2008

Current Topics in Microbiology and Immunology

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Promoting remyelination following injury to the central nervous system (CNS) promises to be an effective neuroprotective strategy to limit the loss of surviving axons and prevent disability. Studies confirm that multiple sclerosis (MS) and spinal cord injury lesions contain myelinating cells and their progenitors. Recruiting these endogenous cells to remyelinate may be of therapeutic value. This review addresses the use of antibodies reactive to CNS antigens to promote remyelination. Antibody-induced remyelination in a virus-mediated model of chronic spinal cord injury was initially observed in response to treatment with CNS reactive antisera. Monoclonal mouse and human IgMs, which bind to the surface of oligodendrocytes and myelin, were later identified that were functionally equivalent to antisera. A recombinant form of a human remyelination-promoting IgM (rHIgM22) targets areas of CNS injury and promotes maximal remyelination within 5 weeks after a single low dose (25 µg/kg). The IgM isoform of this reparative antibody is required for in vivo function. We hypothesize that the IgM clusters membrane domains and

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“…Glycolipids also bind to extracellular matrix molecules such as laminin [14,15] and fibronectin [13], suggesting that a modulatory effect could be possible. Glycolipids have also been involved with axonogenesis and neurite outgrowth [17,44], an effect which has been associated with interactions with growth-promoting components present in the environment [45].…”

Section: C1 Antigen Is Differentially Expressed During Embryonic Devmentioning

confidence: 99%

“…Some glycolipids bind specifically to the extracellular matrix molecules fibronectin [13] and laminin [14,15], while others modify neural cell adhesion to laminin-rich substrates [10]. Glycolipids may act in defining locations of axon tract formation [7], in defining or specifying different compartments within the developing nervous system [16], or during axon extension [9,17].…”

Section: Introductionmentioning

confidence: 99%

Monoclonal Antibody against a Neuronal Antigen Impairs Formation of the Axon Scaffold in Grasshopper Central Nervous System

Bernhardi

Carpenter²

1999

Dev Neurosci

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The pathway a growing axon follows is determined by a number of cues, including differential adhesion to surface molecules on axons and the matrix of the fascicles along which they grow. We have characterized the differential expression of an extracellular antigen and the effects of a monoclonal antibody against this molecule on the development of the grasshopper central nervous system (CNS). The 5C1 monoclonal antibody was generated against ganglion chains of grasshopper embryos; it labels cell bodies of newly differentiated neurons and their axons as they extend. Electron microscopy of embryos at 42% of development reveals that 5C1 labels neuronal filopodia, axons and somata, and areas of glial membrane in apposition to neurite fascicles. After 70% of development, labeling is lost from axon bundles, but remains on cell bodies. 5C1 also cross-reacts with an epitope expressed in Drosophila CNS during embryonic development. Enzymatic digestion suggests that the antigen recognized by the antibody is likely to be a glycolipid. In embryos exposed to 5C1 during early stages of development of the CNS, at the time when the first axons begin to extend, the formation of axon pathways is blocked or greatly delayed. Our results suggest that the 5C1 antigen participates in the formation of the axon scaffold and may play a functional role in the initiation and maintenance of axon outgrowth during early development of the CNS.

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Neurite Outgrowth Is Enhanced by Anti-Idiotypic Monoclonal Antibodies to the Ganglioside GM1

Cited by 5 publications

References 60 publications

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a

Remyelination-Promoting Human IgMs: Developing a Therapeutic Reagent for Demyelinating Disease

Monoclonal Antibody against a Neuronal Antigen Impairs Formation of the Axon Scaffold in Grasshopper Central Nervous System

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Neurite Outgrowth Is Enhanced by Anti-Idiotypic Monoclonal Antibodies to the Ganglioside GM1

Cited by 5 publications

References 60 publications

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findingsa

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findingsa

Remyelination-Promoting Human IgMs: Developing a Therapeutic Reagent for Demyelinating Disease

Monoclonal Antibody against a Neuronal Antigen Impairs Formation of the Axon Scaffold in Grasshopper Central Nervous System

Contact Info

Product

Resources

About

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a

The Role of GM1 and Other Gangliosides in Neuronal Differentiation Overview and New Findings^a