Neurite extension of primary neurons on electrospun piezoelectric scaffolds

Lee, Young Soon; Collins, George; Arinzeh, Treena Livingston

doi:10.1016/j.actbio.2011.07.013

Cited by 176 publications

(179 citation statements)

References 64 publications

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“…Recent papers indicate that electrospinning of poly(vinylidene fluoride trifluorethylene) (PVDF-TrFE) can be performed from polymer dissolution in ethylmethylketone [18,19]. Complete dissolution of the polymer in such solvent is difficult to achieve.…”

Section: Resultsmentioning

confidence: 99%

Fiber average size and distribution dependence on the electrospinning parameters of poly(vinylidene fluoride–trifluoroethylene) membranes for biomedical applications

et al. 2012

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Poly(vinylidene fluoride-trifluoethylene), P(VDF-TrFE), electrospun membranes were obtained from a mixture of dimethylformamide (DMF) and methylethylketone (MEK) solvents. The inclusion of MEK to the solvent system promotes a faster solvent evaporation allowing complete polymer crystallization during the jet travelling between the tip and the grounded collector.The main electrospinning processing parameters were systematically changed to study their influence on fiber dimensions and distribution. Applied voltage and inner needle diameter do not have large influence on the electrospun fiber average diameter but in the fiber diameter distribution. On the other hand, increasing the distance between the needle tip and the collector gives origin to fibers with larger average diameter. Independently on the processing conditions, all mats are produced in the electroactive phase of the polymer. Further, MC-3T3-E1cell adhesion is not inhibited by the fiber mats preparation, showing their potential use for biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Fiber average size and distribution dependence on the electrospinning parameters of poly(vinylidene fluoride–trifluoroethylene) membranes for biomedical applications

et al. 2012

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“…The same cells was also used with PLLA fibers to study their biocompatibility and suitability for bone differentiation and the same results was obtained [130]. Regarding nerve regeneration, fibers was also used and it was verified that the cells attach and the neurites extend radially on the random aligned fibers, whereas the aligned fibers directed the neurite outgrowth, demonstrating their potential for neural tissue engineering [147][148].…”

Section: Tissue Engineer Ing Based On Piezoelectr Ic Polymer Smentioning

confidence: 94%

Piezoelectric polymers as biomaterials for tissue engineering applications

Ribeiro

Sencadas

Correia

et al. 2015

Colloids and Surfaces B: Biointerfaces

403

338

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Tissue engineering often rely on scaffolds for supporting cell differentiation and growth. Novel paradigms for tissue engineering include the need of active or smart scaffolds in order to properly regenerate specific tissues. In particular, as electrical and electromechanical clues are among the most relevant ones in determining tissue functionality in tissues such as muscle and bone, among others, electroactive materials and, in particular, piezoelectric ones, show strong potential for novel tissue engineering strategies, in particular taking also into account the existence of these phenomena within some specific tissues, indicating their requirement also during tissue regeneration. This referee reports on piezoelectric materials used for tissue engineering applications. The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This review provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions. This referee reports on piezoelectric materials used for tissue engineering applications.The most used materials for tissue engineering strategies are reported together with the main achievements, challenges and future needs for research and actual therapies. This referee provides thus a compilation of the most relevant results and strategies and a start point for novel research pathways in the most relevant and challenging open questions.

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“…Electrospun PVDF-TrFE scaffolds having aligned fibers were fabricated and characterized as previously described (Lee and Arinzeh, 2012;Lee et al, 2011b). Briefly, PVDF-TrFE (65/35) (Solvay Solexis, Inc., Cranbury, NJ) was dissolved in methyl ethyl ketone and electrospun using a fast rotating drum to collect aligned fibers.…”

Section: Fabrication and Characterization Of Pvdf-trfe Scaffoldsmentioning

confidence: 99%

The effect of PVDF‐TrFE scaffolds on stem cell derived cardiovascular cells

Hitscherich

Gordan

et al. 2016

Biotech & Bioengineering

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Recently, electrospun polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (PVDF-TrFE) scaffolds have been developed for tissue engineering applications. These materials have piezoelectric activity, wherein they can generate electric charge with minute mechanical deformations. Since the myocardium is an electroactive tissue, the unique feature of a piezoelectric scaffold is attractive for cardiovascular tissue engineering applications. In this study, we examined the cytocompatibility and function of pluripotent stem cell derived cardiovascular cells including mouse embryonic stem cell-derived cardiomyocytes (mES-CM) and endothelial cells (mES-EC) on PVDF-TrFE scaffolds. MES-CM and mES-EC adhered well to PVDF-TrFE and became highly aligned along the fibers. When cultured on scaffolds, mES-CM spontaneously contracted, exhibited well-registered sarcomeres and expressed classic cardiac specific markers such as myosin heavy chain, cardiac troponin T, and connexin43. Moreover, mES-CM cultured on PVDF-TrFE scaffolds responded to exogenous electrical pacing and exhibited intracellular calcium handling behavior similar to that of mES-CM cultured in 2D. Similar to cardiomyocytes, mES-EC also demonstrated high viability and maintained a mature phenotype through uptake of low-density lipoprotein and expression of classic endothelial cell markers including platelet endothelial cell adhesion molecule, endothelial nitric oxide synthase, and the arterial specific marker, Notch-1. This study demonstrates the feasibility of PVDF-TrFE scaffold as a candidate material for developing engineered cardiovascular tissues utilizing stem cell-derived cells. Biotechnol. Bioeng. 2016;113: 1577-1585. © 2015 Wiley Periodicals, Inc.

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Neurite extension of primary neurons on electrospun piezoelectric scaffolds

Cited by 176 publications

References 64 publications

Fiber average size and distribution dependence on the electrospinning parameters of poly(vinylidene fluoride–trifluoroethylene) membranes for biomedical applications

Fiber average size and distribution dependence on the electrospinning parameters of poly(vinylidene fluoride–trifluoroethylene) membranes for biomedical applications

Piezoelectric polymers as biomaterials for tissue engineering applications

The effect of PVDF‐TrFE scaffolds on stem cell derived cardiovascular cells

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