Neural Stem Cells Harvested from Live Brains by Antibody‐Conjugated Magnetic Nanoparticles

Lui, Cathy N. P.; Tsui, Yat-Ping; Ho, A.; Shum, Dky; Chan, Ying-Shing; Wu, Ching-Tang; Li, Hung‐Wing; Tsang, Shik Chi Edman; Yung, Kin Lam

doi:10.1002/anie.201305482

Cited by 24 publications

(20 citation statements)

References 19 publications

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“…The biocompatibility and biodegradability of MNPs featuring unique magnetic and heat dissipation characteristics are central to their successful uses in several in vitro and in vivo applications 5. The utilization of monoclonal antibody (mAb)–MNP nanoconjugates for whole blood purification,6 controlling cancer-cell death,7 tumor targeting,8 neural stem cell extraction,9 and the detection of biomarkers10–12 and bacterial cells13 is becoming more common. mAbs offer exquisitely specific and sensitive antigen recognition in such applications, and therefore, strategies for the functionalization of MNP surfaces with Abs are essential for the utilization of MNPs in high-performance immunodiagnostic technologies.…”

Section: Introductionmentioning

confidence: 99%

“…Proteins adsorbed onto MNP surfaces through hydrophobic and polar interactions in random orientations are not sufficiently stable for many applications. One of the most popular methods for the covalent conjugation of MNPs with Abs involves carbodiimide-based coupling6,9–13 between carboxyl groups on the MNP and the most reactive side chain ε-amines of lysine residues of the Ab, which are positioned at various locations on the surface of the Ab. Despite the lack of Ab modification, this strategy can compromise Ab activity due to potential blockade of the Fab site as a result of attachment to a residue at the Fab site or of nondirectional coupling to the NP surface.…”

Section: Introductionmentioning

confidence: 99%

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Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

et al. 2019

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“…Magnetic nanoparticles made up of iron oxide and conjugated with CD133 were successfully used for isolation of NSC from ependymal cells of adult rats. 154 All rats remained alive and healthy after the procedure and cells extracted were found to be capable of neuronal differentiation.…”

Section: Advances In Polymer Chemistry and Nanoparticle Delivery For mentioning

confidence: 96%

Enabling nanomaterial, nanofabrication and cellular technologies for nanoneuromedicines

Mallapragada

Brenza

McMillan

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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Nanoparticulate delivery systems represent an area of particular promise for nanoneuromedicines. They possess significant potential for desperately needed therapies designed to combat a range of disorders associated with aging. As such, the field was selected as the focus for the 2014 meeting of the American Society for Nanomedicine. Regenerative, protective, immune modulatory, anti-microbial and anti-inflammatory products, or imaging agents are readily encapsulated in or conjugated to nanoparticles and as such facilitate the delivery of drug payloads to specific action sites across the blood-brain barrier. Diagnostic imaging serves to precisely monitor disease onset and progression while neural stem cell replacement can regenerate damaged tissue through control of stem cell fates. These, taken together, can improve disease burden and limit systemic toxicities. Such enabling technologies serve to protect the nervous system against a broad range of degenerative, traumatic, metabolic, infectious and immune disorders.

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“…Stem cells from the endothelial lining along with the MNPs were released and collected with a syringe or by a magnet probe. 164 Protein A coated magnetic nanoparticles are exploited in preparatory refinement of antibodies (IgGs) from a cell culture. 165 DNA adsorption and desorption mechanism on temperaturesensitive PNIPAM-co-AEMH (poly (N-isopropylacrylamide-co-aminoethylmethacrylate)) coated magnetic nanoparticles were consumed in separation microsystem for extracting, refining, and concentrating nucleic acid.…”

Section: Magneticmentioning

confidence: 99%

Magnetic Nanoparticles: From Synthesis to Theranostic Applications

Khizar

Ahmad

Zine

et al. 2021

ACS Appl. Nano Mater.

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Over the past few years, engineered inorganic nanoparticles have been studied researched, and applied extensively in the biomedical field since resultant platforms increase the usage of the nanoparticles for particular theranostic applications. The innovation of such nanosystems has the potential to develop a theranostic mode that integrates both diagnosis and treatment of diseases in a particular system via combinatorial approaches of imaging, targeting, and therapy that accomplish the potential of personalized and tailored medicine. The manipulation of magnetic nanoparticles (MNPs) as theranostic agents have attained growing consideration in material science owing to their exclusive capability in magnetic targeting, magnetic resonance imaging, chemotherapy, hyperthermia, bioseparation, gene therapy, enzyme immobilization, and controlled release of the drug. To intensify the diagnostic and therapeutic efficacy, MNPs have been ornamented or functionalized with a range of materials to enhance the biocompatibility, stability, and capability to carry therapeutic payloads and to encapsulate imaging agents. Preferentially synthetic and natural polymers have been exploited to coat for ensuring their colloidal stability and good dispersibility in biological fluids. Stimuli-responsive polymers have also been used to functionalize MNPs to establish a therapeutic approach that can significantly enhance therapeutic effectiveness including the real-time monitoring in specific cells and tissues. The present review highlights the progress of MNPs emphasizing functionalization using diverse inorganic and organic and biomaterials. Furthermore, it also provides the soundproof concept on the potential of MNPs and their colloidal counterparts that offer attractive possibilities for theranostic applications. Finally, the main challenges and limitations in the use of MNPs for advanced biomedical applications have also been critically provided.

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Neural Stem Cells Harvested from Live Brains by Antibody‐Conjugated Magnetic Nanoparticles

Cited by 24 publications

References 19 publications

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

Enabling nanomaterial, nanofabrication and cellular technologies for nanoneuromedicines

Magnetic Nanoparticles: From Synthesis to Theranostic Applications

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