Neural Stem Cell– and Schwann Cell–Loaded Biodegradable Polymer Scaffolds Support Axonal Regeneration in the Transected Spinal Cord

Olson, Heather E.; Rooney, Gemma E.; Gross, LouAnn; Nesbitt, Jarred J.; Galvin, Katherine E.; Knight, Andrew M.; Chen, BingKun; Yaszemski, Michael J.; Windebank, Anthony J.

doi:10.1089/ten.tea.2008.0364

Cited by 152 publications

(156 citation statements)

References 36 publications

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“…Previous research using a simple PGA conduit has evolved into a veritable number of types and combinations to produce the best synthetic nerve conduit [17]. Researchers are currently studying the benefits of filling these conduits with biological material such as collagen or with neurotrophic factors to further enhance the potential of nerve regeneration [18,19]. This vast variability in producing a synthetic nerve conduit has resulted in no clear consensus on the best product available.…”

Section: Discussionmentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit*

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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Background. Autologous nerve grafts to bridge nerve gaps pose various drawbacks. Nerve tissue engineering to promote nerve regeneration using artificial neural conduits has emerged as a promising alternative. Objectives. To develop an artificial nerve conduit using collagen-coated polylactic-glycolic acid (PLGA) and to analyse the survivability and propagating ability of the neuro-differentiated human mesenchymal stem cells in this conduit. Material and Methods. The PLGA conduit was constructed by dip-molding method and coated with collagen by immersing the conduit in collagen bath. The ultra structure of the conduits were examined before they were seeded with neural-differentiated human mesenchymal stem cells (nMSC) and implanted sub-muscularly on nude mice thighs. The non-collagen-coated PLGA conduit seeded with nMSC and non-seeded non-collagen-coated PLGA conduit were also implanted for comparison purposes. The survivability and propagation ability of nMSC was studied by histological and immunohistochemical analysis. Results. The collagen-coated conduits had a smooth inner wall and a highly porous outer wall. Conduits coated with collagen and seeded with nMSCs produced the most number of cells after 3 weeks. The best conduit based on the number of cells contained within it after 3 weeks was the collagen-coated PLGA conduit seeded with neuro-transdifferentiated cells. The collagen-coated PLGA conduit found to be suitable for attachment, survival and proliferation of the nMSC. Minimal cell infiltration was found in the implanted conduits where nearly all of the cells found in the cell seeded conduits are non-mouse origin and have neural cell markers, which exhibit the biocompatibility of the conduits. Conclusions. The collagen-coated PLGA conduit is biocompatible, non-cytotoxic and suitable for use as artificial nerve conduits (Adv Clin Exp Med 2014, 23, 3, 353-362).

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Section: Discussionmentioning

confidence: 99%

Collagen-Coated Polylactic-Glycolic Acid (PLGA) Seeded with Neural-Differentiated Human Mesenchymal Stem Cells as a Potential Nerve Conduit*

Sulong¹,

Hassan²,

Ng³

et al. 2014

Adv Clin Exp Med

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“…Both NSC-and Schwann cell-seeded scaffold treated groups showed greater axonal counts than the unseeded control group, with the Schwann cell seeded scaffold treated group having the most axonal counts, although the absolute numbers of axonal counts of all groups were relatively small compared with that of normal spinal cord. They found no significant difference in locomotor function recovery in all groups [218].…”

Section: Neural Stem Cellsmentioning

confidence: 86%

“…The effect of NSCs and Schwann cells on axonal regeneration was studied by Olson et al [218]. They fabricated a multi-channel PLGA scaffold seeded with either NSCs or Schwann cells and implanted in the transected spinal cord of rats.…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Han

Cheung

2011

Polymers

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Central and peripheral neural injuries are traumatic and can lead to loss of motor and sensory function, chronic pain, and permanent disability. Strategies that bridge the site of injury and allow axonal regeneration promise to have a large impact on restoring quality of life for these patients. Engineered materials can be used to guide axonal growth. Specifically, nanofiber structures can mimic the natural extracellular matrix, and aligned nanofibers have been shown to direct neurite outgrowth and support axon regeneration. In addition, cell-seeded scaffolds can assist in the remyelination of the regenerating axons. The electrospinning process allows control over fiber diameter, alignment, porosity, and morphology. Biodegradable polymers have been electrospun and their use in tissue engineering has been demonstrated. This paper discusses aspects of electrospun biodegradable nanofibers for neural regeneration, how fiber alignment affects cell alignment, and how cell-seeded scaffolds can increase the effectiveness of such implants.

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“…In fact, PLGA 10 : 90 (Vicryl ® , Ethicon) and the copolymer LA/GA ratio 90 : 10 (Panacryl ® , Ethicon) are used as suture materials [114,115] and microspheres, microcapsules, nanospheres, or nanofibers of PLGA are available to deliver chemotherapeutics [116,117], proteins [118], vaccines [119], antibiotics [120], analgesics [121], antiinflammatory [122], and siRNA [123,124]. Moreover, PLGA demonstrates great cell adhesion and good proliferation properties making it an excellent candidate for application in tissue engineering, as shown by the available scaffolds for the engineering of bone [125,126], cartilage [127], tendon [128] [129], skin [130], liver [131], and nerve tissues [132]. PLGA scaffolds are also used to regenerate damaged tissues in regenerative dentistry, together with stem cell-based therapy.…”

Section: Polyestersmentioning

confidence: 99%