Neural Cell Adhesion Molecule Regulates Osteoblastic Differentiation Through Wnt/β-Catenin and PI3K-Akt Signaling Pathways in MC3T3-E1 Cells

Cheng, Bin-Feng; Feng, Xiao; Gao, Yao-Xin; Jian, Shao-Qin; Liu, Shi-Rao; Wang, Mian; Xie, Yunfei; Feng, Zhiwei; Yang, Haijun

doi:10.3389/fendo.2021.657953

Cited by 14 publications

(18 citation statements)

References 42 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple studies have shown that the expression of Wnt6, Wnt10a, and Wnt10b inhibits the differentiation of MSCs into adipocytes while promoting MSCs differentiation into osteoblasts through the canonical Wnt pathway [34]. Furthermore, lots of studies have revealed that Wnt/β-catenin plays an important role in the proliferation and differentiation of embryonic osteoblasts [35,36]. Met also activated the Wnt/βcatenin signaling pathway to reduce inflammation and apoptosis, according to Zhang et al [37].…”

Section: Discussionmentioning

confidence: 99%

Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis

Huang

Liang

et al. 2022

BMC Endocr Disord

View full text Add to dashboard Cite

Background With the deepening of social aging, the incidence rate of osteoporosis and diabetes continues to rise. More and more clinical studies show that diabetes is highly correlated with osteoporosis. Diabetes osteoporosis is considered as a metabolic bone disease of diabetes patients. This study aims to explore the role and mechanism of metformin (Met) in diabetic osteoporosis. Methods Mouse MC3T3-E1 cells were treated with Met (0.5 mM) and exposed to high glucose (HG, 35 mM). The cells were cultured in an osteogenic medium for osteogenic differentiation, and the cell proliferation ability was determined using Cell Counting Kit-8; Alkaline phosphatase (ALP) activity detection and alizarin red staining were utilized to evaluate the effect of Met on MC3T3-E1 osteogenic differentiation. Western blot was used to detect the expressions of osteogenesis-related proteins (Runx2 and OCN) as well as Wnt/β-catenin signaling pathway-related proteins in MC3T3-E1 cells. Results HG inhibited proliferation and calcification of MC3T3-E1 cells, down-regulated ALP activity, and the expression of Runx2 and OCN in MC3T3-E1 cells. Meanwhile, the activity of the Wnt/β-catenin signaling pathway was inhibited. Met treatment was found to significantly stimulate the proliferation and calcification of MC3T3-E1 cells under HG conditions, as well as increase the ALP activity and the protein expression level of Runx2 and OCN in the cells. As a result, osteogenic differentiation was promoted and osteoporosis was alleviated. Apart from this, Met also increased the protein expression level of Wnt1, β-catenin, and C-myc to activate the Wnt/β-catenin signaling pathway. Conclusion Met can stimulate the proliferation and osteogenic differentiation of MC3T3-E1 cells under HG conditions. Met may also treat diabetic osteoporosis through Wnt/β-catenin activation.

show abstract

Section: Discussionmentioning

confidence: 99%

Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis

Huang

Liang

et al. 2022

BMC Endocr Disord

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that neural cell adhesion molecule silencing inhibits osteoblast differentiation by inactivating Wnt/β-catenin and PI3K-Akt signaling pathways. CD137 may be involved in p53 and Wnt/β-catenin signaling pathways, thereby regulating the imbalance of bone homeostasis and affecting bone mass ( 53 , 54 ). We hypothesized that adhesion molecules may be associated with an imbalance in bone homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Identification of hub genes for early detection of bone metastasis in breast cancer

Zhao

Yang

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

BackgroundGlobally, among all women, the most frequently detected and diagnosed and the most lethal type of cancer is breast cancer (BC). In particular, bone is one of the most frequent distant metastases 24in breast cancer patients and bone metastasis arises in approximately 80% of advanced patients. Thus, we need to identify and validate early detection markers that can differentiate metastasis from non-metastasis breast cancers.MethodsGSE55715, GSE103357, and GSE146661 gene expression profiling data were downloaded from the GEO database. There was 14 breast cancer with bone metastasis samples and 8 breast cancer tissue samples. GEO2R was used to screen for differentially expressed genes (DEGs). The volcano plots, Venn diagrams, and annular heatmap were generated by using the ggplot2 package. By using the cluster Profiler R package, KEGG and GO enrichment analyses of DEGs were conducted. Through PPI network construction using the STRING database, key hub genes were identified by cytoHubba. Finally, K-M survival and ROC curves were generated to validate hub gene expression.ResultsBy GO enrichment analysis, 143 DEGs were enriched in the following GO terms: extracellular structure organization, extracellular matrix organization, leukocyte migration class II protein complex, collagen tridermic protein complex, extracellular matrix structural constituent, growth factor binding, and platelet-derived growth factor binding. In the KEGG pathway enrichment analysis, DEGs were enriched in Staphylococcus aureus infection, Complement and coagulation cascades, and Asthma. By PPI network analysis, we selected the top 10 genes, including SLCO2B1, STAB1, SERPING1, HLA-DOA, AIF1, GIMAP4, C1orf162, HLA-DMB, ADAP2, and HAVCR2. By using TCGA and THPA databases, we validated 2 genes, SERPING1 and GIMAP4, that were related to the early detection of bone metastasis in BC.Conclusions2 abnormally expressed hub genes could play a pivotal role in the breast cancer with bone metastasis by affecting bone homeostasis imbalance in the bone microenvironment.

show abstract

“…The copyright holder for this preprint this version posted March 20, 2023. ; https://doi.org/10.1101/2023.03.20.533133 doi: bioRxiv preprint (20), and that its presence can regulate chondrocyte hypertrophy in chromogenic differentiation (18). The protein Fc fragment of IgG receptor IIIb (FCGR3B) is involved in the immune response and are known to bind to IgG, and has been detected to be more abundant in menisci with OA than in healthy menisci (21).…”

Section: Discussionmentioning

confidence: 99%

Altered co-expression patterns of synovial fluid proteins related to the immune system and extracellular matrix organization in late stage OA, compared to non-OA controls

Lonsjo

Rydén

Turkiewicz

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective Synovial fluid contains proteins that may have been released from surrounding tissues, our aim was to gain new insights into the proteomic profiles of human synovial fluid in knees with and without osteoarthritis (OA). Methods We used synovial fluid from 11 patients with end-stage medial compartment knee OA, aspirated during total knee replacement, and from 13 deceased donors who had no prior history of knee OA (healthy controls). These samples were analyzed using high-multiplex immunoassays. The differential expression of proteins between the groups was analyzed using a linear mixed effects model. The linear associations between pairs of protein expressions were estimated with a linear regression model. Results We found that almost half of the detected proteins were differentially expressed between the OA and non-OA controls. The proteins that were most elevated in the OA group compared to controls were tartrate-resistant acid phosphatase type 5 (fold change 10.6, 95% CI [6.6-17.0]), coagulation factor XI (4.3 [2.6-6.8]) and urokinase-type plasminogen activator (4.3 [2.3-6.8]). The proteins with lower levels in OA compared to controls were fatty acid-binding protein, adipocyte (0.03 [0.02-0.05]), myocilin (0.05 [0.03-0.08]) and carbonic anhydrase 3 (0.14 [0.09-0.23]). The protein-protein co-expression analysis suggests an overall lower number of protein pairs that show co-expression in OA. Conclusion There is a substantial change in protein abundance in synovial fluid in end-stage knee OA, suggesting that global joint homeostasis is severely deranged. Our findings suggest altered co-expression between the immune response and extracellular matrix organization in end-stage knee OA, in comparison to non-OA controls.

show abstract

Neural Cell Adhesion Molecule Regulates Osteoblastic Differentiation Through Wnt/β-Catenin and PI3K-Akt Signaling Pathways in MC3T3-E1 Cells

Cited by 14 publications

References 42 publications

Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis

Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis

Identification of hub genes for early detection of bone metastasis in breast cancer

Altered co-expression patterns of synovial fluid proteins related to the immune system and extracellular matrix organization in late stage OA, compared to non-OA controls

Contact Info

Product

Resources

About