Neuartige Alkenylether‐Lipide aus anaeroben Bakterien

Thiele, O. W.; Oulevey, J.; Bahl, Hubert

doi:10.1002/lipi.19850871315

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…In particular the wide diversity of glycolipids documented here is not present in those strains assigned to Clostridium cluster I. In addition, the presence of phosphatidylethanolamine in all the strains of Clostridium cluster I examined in previous studies (Goldfine & Johnston, 2005;Guan et al, 2011Guan et al, , 2012Guan et al, , 2013Johnston & Goldfine, 1983;Johnston et al, 2004Johnston et al, , 2010Oulevey et al, 1986;Thiele et al, 1985;Tian et al, 2013) clearly distinguishes them from the strains examined here that do not synthesize phosphatidylethanolamine, which was supported by the absence of phospholipids with free amino groups in the polar lipid analyses. Care should, however, be taken with regard to the conclusions drawn in some of these publications because links are made to some strains that in the past were incorrectly identified.…”

mentioning

confidence: 51%

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

Gerritsen

Fuentes

Grievink

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

275

191

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A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C12–C19 fatty acids, with C16 : 0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1σ lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium , including Clostridium lituseburense (97.2 %), Clostridium glycolicum (96.2 %), Clostridium mayombei (96.2 %), Clostridium bartlettii (96.0 %) and Clostridium irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (<85 %) to the type strain of Clostridium butyricum , the type species of the genus Clostridium . DNA–DNA hybridization with closely related reference strains indicated reassociation values below 32 %. On the basis of phenotypic and genetic studies, a novel genus, Romboutsia gen. nov., is proposed. The novel isolate CRIBT ( = DSM 25109T = NIZO 4048T) is proposed as the type strain of the type species, Romboutsia ilealis gen. nov., sp. nov., of the proposed novel genus. It is proposed that C. lituseburense is transferred to this genus as Romboutsia lituseburensis comb. nov. Furthermore, the reclassification into novel genera is proposed for C. bartlettii , as Intestinibacter bartlettii gen. nov., comb. nov. (type species of the genus), C. glycolicum , as Terrisporobacter glycolicus gen. nov., comb. nov. (type species of the genus), C. mayombei , as Terrisporobacter mayombei gen. nov., comb. nov., and C. irregulare , as Asaccharospora irregularis gen. nov., comb. nov. (type species of the genus), on the basis of additional data collected in this study. In addition, an emendation of the species Peptostreptococcus anaerobius and the order Eubacteriales is provided.

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mentioning

confidence: 51%

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

Gerritsen

Fuentes

Grievink

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

275

191

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“…Similar changes in GAPlaE relative to the content of other polar lipids have been observed in C. acetobutylicum, which produces acetone and butanol (20). The lipids in both organisms are similar with the exception of the presence of larger amounts of glycolipids in the latter species, especially in low-phosphate media (30,34). The methods we have used with C. butyricum, which involve growth in the presence of a single fatty acid supplement and inhibition of endogenous fatty acid synthesis, have allowed us to study the regulation of polar lipid composition in response to solvents in the absence of changes in lipid aliphatic chains.…”

Section: Resultsmentioning

confidence: 99%

Effects of solvents and alcohols on the polar lipid composition of Clostridium butyricum under conditions of controlled lipid chain composition

MacDonald

Goldfine

1991

Appl Environ Microbiol

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Clostridium bulyricum has been grown in media devoid of biotin, to which long-chain fatty acids have been added to promote growth. We have shown previously that, under these conditions, exogenous fatty acids are extensively incorporated into the cellular phospholipids. Cells grown with elaidic acid, trans-9-18:1, have normal ratios of the glycerol acetal of plasmenylethanolamine (GAPlaE) to phosphatidylethanolamine (PE) plus plasmenylethanolamine (PlaE) compared with cells grown with biotin. When ethanol, cyclohexane, or n-octanol was added to elaidate-containing media, the ratio of GAPIaE to PE plus PlaE was significantly increased. Addition of dodecane and n-butanol did not affect this ratio. When cells were grown with oleic acid in the absence of biotin, the GAPlaE to PE plus PlaE ratio was increased 5.4-fold compared with elaidate-grown cells. In oleate-supplemented media, the addition of solvents or n-alcohols produced no further increase in this ratio. We conclude that these changes in lipid composition represent cellular responses to perturbation of the equilibria between the lamellar and nonlamellar liquid crystalline phases in the cell membrane.

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“…Thus it appears that an Etn-P modification of membrane glycolipids is a recurrent theme among clostridia. We have not investigated the identities of the sugars in EtnP-MGDRG and EtnP-DGDRG, but we assume they are derivatives of the previously reported galactosyl-DRG and glucosyl-galactosyl-DRG of C. acetobutylicum [21,22]. …”

Section: Discussionmentioning

confidence: 99%

An ethanolamine-phosphate modified glycolipid in Clostridium acetobutylicum that responds to membrane stress

Teng

Guan

Goldfine

2013

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Two phosphorus-containing glycolipids have previously been observed in Clostridium acetobutylicum. We had shown that the concentration of one of them increases in response to increased unsaturation of the membrane lipid hydrocarbon chains, suggesting a potential role in the regulation of lipid polymorphism in this organism. Mass spectrometry shows that these glycolipids are ethanolamine phosphate (Etn-P)-containing derivatives of a mono- and diglycosyldiradylglycerol. The content of both diglycosyldiradylglycerol and the Etn-P-monoglycosyldiradylglycerol, which increase upon increased unsaturation of the membrane, also increase upon addition of octanol to the medium. Thus, it appears that the Etn-P-monoglycosyldiradylglycerol along with the diglycosyldiradylglycerol may serve to stabilize the membrane bilayer during membrane stress caused by the presence of the solvents produced during fermentation.

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Neuartige Alkenylether‐Lipide aus anaeroben Bakterien

Cited by 7 publications

References 22 publications

Effects of solvents and alcohols on the polar lipid composition of Clostridium butyricum under conditions of controlled lipid chain composition

An ethanolamine-phosphate modified glycolipid in Clostridium acetobutylicum that responds to membrane stress

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