Network regulation among T cells; Conclusions from limiting dilution experiments

The Tetramer Transformation

“…In general, ,1:100-1:1000 CD8 1 T cells were thought to be responders. Even so, the LDA approach did provide rigorous evidence to support the, at times, disputed (7,8) view that virus-specific CD8…”

Some problem areas in the interaction between viruses and the immune system

“…There is little doubt that this reflects proliferation, not simply release from suppression as some have argued (35)(36)(37)(38). The T cells then enter blood or lymph and localise to sites of virus growth.…”

Quantitating Effector and Regulatory T Lymphocytes in Immune Responses by Limiting Dilution Analysis Modeling

“…In a variety of LD experiments, ⌫ was found to be in order from 5 to 20, with the value of a ranging from 5 to 10, assuming that there was rather a large number of regulatory cells required for suppression of one proliferating cell. Moreover, the results of the modeling studies suggested a notable excess of T reg1 cells relatively to proliferating T E cells, with ( f r1 /f e ) Ͼ 20 (52)(53)(54). However, these data must be currently considered cautiously.…”

“…A͑n͒ ϭ a ϩ y͑n͒ (9) with y(n) ϭ ͌ ⌫n, or y(n) ϭ ⌫n, or y(n) ϭ ⌫ ͌ n (52)(53)(54). This function means that the minimum number A(n) of T reg1 cells that must be simultaneously present with n proliferating cells in the same well to suppress their growth, is not directly proportional to n as described in model 1, which assumes T reg1 ϭ ⌽T e (corresponding to Equation 3).…”