Network Pharmacology-Based Strategy for Predicting Therapy Targets of Tripterygium wilfordii on Acute Myeloid Leukemia

Abstract

Background. Acute myeloid leukemia (AML) is a common malignant tumor of the hematopoietic system. How to extend the survival time of AML patients and improve their prognosis is still a major medical problem. Chinese medicine has a long history in treating AML. Tripterygium wilfordii (TW) is a traditional Chinese medicine. With the deepening of pharmacological research of traditional Chinese medicine, triptolide, one of its active ingredients, has been proven to have a positive effect on the treatment of AML. Therefore,this study aimed on studying the potential therapeutic targets and pharmacological mechanism of TW in Acute myeloid leukemia (AML) based on network pharmacology. Methods. The active components of TW were obtained by network pharmacology through oral bioavailability, drug-likeness filtration. Comparative analysis was used to study the overlapping genes between active ingredient’s targets and AML treatment-related targets. Using STRING database to analyze interactions between overlapping genes. KEGG pathway analysis and Gene Ontology enrichment analysis were conducted in DAVID. These genes were analyzed for survival in OncoLnc database. Results. We screened 53 active ingredients, the results of comparative analysis showed that 8 active ingredients had an effect on AML treatment. Based on the active ingredients and overlapping genes, we constructed the Drug-Compounds-Genes-Disease Network. Survival analysis of overlapping genes indicated that some targets possess a significant influence on patients’ survival and prognosis. The enrichment analysis showed that the main pathways of targets are Toll-like receptor signaling pathway, NF-kappa B signaling pathway and HIF-1 signaling pathway. Conclusion. This study, using a network pharmacologic approach, provides another strategy that can help us to understand the mechanisms by which TW treats AML comprehensively.

Combination of UHPLC‐Q Exactive‐Orbitrap MS and network pharmacology to reveal the mechanism of Eucommia ulmoides leaves in the treatment of osteoarthritis

“…Its characteristics of integrity, systematicness, and emphasis on drug interaction are consistent with the features of TCM. It has been successfully applied to the prediction of therapeutic mechanisms in various TCM such as Tripterygium wilfordii (Fang et al, 2020), Rhizoma Coptidis (An et al, 2021), and Huang-Q i-Gui-Zhi-Wu-Wu Decoction (Gu et al, 2020).…”

Network Pharmacology-Based Strategy and Molecular Docking to Explore the Potential Mechanism of Jintiange Capsule for Treating Osteoporosis

“…Although several western medicines, such as bisphosphonates, calcitonin, and estrogen, generate a good effect on OP, the important role of TCM could not be ignored. It is wellknown that TCM has been utilizing in China for over 2500 years [11]. Jintiange capsule, the biomimetic medicine prepared from the artificial tiger bone powders, has been recommended to be the efficient therapy by the treatment guidelines for primary OP and OP-related fracture in China in 2017.…”