Network-based gene expression analysis of intracranial aneurysm tissue reveals role of antigen presenting cells

Krischek, Boris; Kasuya, Hidetoshi; Tajima, Atsushi; Akagawa, Hiroyuki; Sasaki, Takuto; Yoneyama, Taku; Ujiié, Hiroshi; Kubo, Osami; Bonin, Michael; Takakura, Kenji; Hori, Tomokatsu; Inoue, Ituro

doi:10.1016/j.neuroscience.2008.04.049

Cited by 76 publications

(63 citation statements)

References 31 publications

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“…Second, the number of samples was sufficient to use ANOVA. Finally, the ethnicity of patients might influence the final results of the studies (Finnish and black, 2 Japanese, 3 heterogeneous group of Asians, white, Hispanic, 4 and Polish [present report]).…”

Section: Discussionmentioning

confidence: 99%

“…The gene microarray method allows for simultaneous analysis of the expression of thousands of genes. We are aware of only 3 published studies [2][3][4] in which the microarray technique was used for profiling gene expression in human IA. In one study, samples were obtained from autopsies and were pooled.…”

mentioning

confidence: 99%

“…In one study, samples were obtained from autopsies and were pooled. 2 One study was performed in a Japanese population, 3 in which the risk of IA rupture is one of the highest in the world. 5 In the third study, the analyzed population was ethnically heterogeneous.…”

mentioning

confidence: 99%

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Gene Expression Profiles in Human Ruptured and Unruptured Intracranial Aneurysms

Pera

Korostyński

Krzyszkowski

et al. 2010

Stroke

125

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Background and Purpose-Mechanisms underlying development and rupture of intracranial aneurysms (IA) are poorly recognized. The majority of studies on human tissue have focused on predefined pathways. We sought to analyze global gene expression patterns of ruptured IA, unruptured IA, and control vessels. Methods-Transcription profiles were studied in human ruptured (nϭ8) and unruptured (nϭ6) IA, as well as in control intracranial arteries (nϭ5), using oligonucleotide microarrays. Real-time reverse-transcription polymerase chain reaction was used for confirmation. Functional analysis for determination of over-represented ontological groups among gene expression profiles was also performed. Results-The expression of 159 genes differed among the studied groups. Compared to the controls, 131 genes showed common directions of change in both IA groups. The most impacted biological processes for IA are: (1) the muscle system; (2) cell adhesion (downregulation); and (3) the immune system and inflammatory response (upregulation). Ruptured and unruptured IA differed in genes involved in immune/inflammatory processes; expression was reduced in ruptured IA. Conclusions-Decreased expression of genes related to muscle system and cell adhesion is important for the development of IA.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Gene Expression Profiles in Human Ruptured and Unruptured Intracranial Aneurysms

Pera

Korostyński

Krzyszkowski

et al. 2010

Stroke

125

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“…Next, Krischek et al (2008) conducted a networked-based gene expression analysis of IA tissue using oligonucleotide microarrays. They examined six ruptured and four unruptured aneurysms and compared these with control intracranial arteries (obtained from feeding arteries to arteriovenous malformations at the time of craniotomy).…”

Section: Gene Expression Profilementioning

confidence: 99%

Biology of Intracranial Aneurysms: Role of Inflammation

Chalouhi

Ali

Jabbour

et al. 2012

J Cereb Blood Flow Metab

430

418

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Intracranial aneurysms (IAs) linger as a potentially devastating clinical problem. Despite intense investigation, our understanding of the mechanisms leading to aneurysm development, progression and rupture remain incompletely defined. An accumulating body of evidence implicates inflammation as a critical contributor to aneurysm pathogenesis. Intracranial aneurysm formation and progression appear to result from endothelial dysfunction, a mounting inflammatory response, and vascular smooth muscle cell phenotypic modulation producing a pro-inflammatory phenotype. A later final common pathway appears to involve apoptosis of cellular constituents of the vessel wall. These changes result in degradation of the integrity of the vascular wall leading to aneurysmal dilation, progression and eventual rupture in certain aneurysms. Various aspects of the inflammatory response have been investigated as contributors to IA pathogenesis including leukocytes, complement, immunoglobulins, cytokines, and other humoral mediators. Furthermore, gene expression profiling of IA compared with control arteries has prominently featured differential expression of genes involved with immune response/inflammation. Preliminary data suggest that therapies targeting the inflammatory response may have efficacy in the future treatment of IA. Further investigation, however, is necessary to elucidate the precise role of inflammation in IA pathogenesis, which can be exploited to improve the prognosis of patients harboring IA.

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“…1 Back in May 2008 we published our gene expression micorarray study results of 4 unruptured and 6 ruptured aneurysms, as well as 4 arteriovenous malformation feeders which were obtained in a similar way. 2 We used the same pathway analysis program "Ingenuity Pathway Analysis"; the microarray platform used was an Agilent Human Oligo Microarray which carries 18 716 transcripts of human gene expressions. Interestingly, we obtained similar results: our top-scoring networks displayed high-level functions in immune response, cell to cell signaling and interaction, and immune and lymphatic system development and function.…”

Section: To the Editormentioning

confidence: 99%

Gene Expression Microarray Studies of Intracranial Aneurysm Walls Lead to Similar Results

Krischek

Kasuya

2009

Stroke

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Network-based gene expression analysis of intracranial aneurysm tissue reveals role of antigen presenting cells

Cited by 76 publications

References 31 publications

Gene Expression Profiles in Human Ruptured and Unruptured Intracranial Aneurysms

Gene Expression Profiles in Human Ruptured and Unruptured Intracranial Aneurysms

Biology of Intracranial Aneurysms: Role of Inflammation

Gene Expression Microarray Studies of Intracranial Aneurysm Walls Lead to Similar Results

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