Gene Expression Microarray Studies of Intracranial Aneurysm Walls Lead to Similar Results

Krischek, Boris; Kasuya, Hidetoshi

doi:10.1161/strokeaha.109.551978

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…Several previous studies reported the presence of inflammatory cells in the wall of intracranial aneurysms[23, 24]. Histologic analysis of human, intracranial aneurysms using electron microscopy identified the presence of extracellular lysosome-like granules and leukocytes near disorganized elastic lamina, suggesting the presence of degradation of elastic lamina by lytic enzymes secreted by leukocytes in the vascular wall [24, 25].…”

Section: Discussionmentioning

confidence: 96%

Gene Expression Changes: Five Years after Creation of Elastase-induced Aneurysms

Kadirvel

Ding

Dai

et al. 2011

Journal of Vascular and Interventional Radiology

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Purpose Intracranial, saccular aneurysms are associated with chronic remodeling of the arterial wall. The pathobiology of aneurysm growth and rupture is poorly understood. The objective of the present study was to study the gene expression patterns in elastase-induced saccular aneurysms in rabbits five years after aneurysm creation as compared to control, unoperated arteries. Materials and Methods Elastase-induced saccular aneurysms were created in 25 rabbits and followed up to five years. Thirteen rabbits died during follow-up for reasons unrelated to the aneurysms. RNA was isolated from aneurysm tissue and the control contralateral common carotid artery in 5 of the 12 surviving subjects and analyzed for gene expression using human gene microarrays. Genes with statistical differences between groups (p<0.05 and fold change ≥1.5 and ≤0.75) were considered differentially expressed. RT-PCR was used for confirmation of gene microarray findings for selected genes. Results Fifty (0.33%) of 13,353 genes were differentially expressed in the aneurysms compared with the unoperated control arteries. Molecular and functional pathway analysis revealed that immunoregulatory molecules, growth factors, cell adhesion molecules, and structural molecules were differentially expressed in the aneurysms as compared to controls. RT-PCR results of selected genes confirmed the differential expression identified using the gene chip microarray. Conclusions Significant modulation in a variety of biochemical and cellular functions in chronic aneurysms provides molecular insights into the pathophysiology of saccular aneurysms.

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