Network analyses of differentially expressed proteins in amniotic fluid supernatant associated with abnormal human karyotypes

Wang, Tzu‐Hao; Chao, An‐Shine; Chen, Jen‐Kun; Chao, Angel; Chang, Yao‐Lung; Cheng, Po‐Jen; Chang, Shuenn‐Dyh; Wang, Hsin-Shih

doi:10.1016/j.fertnstert.2008.05.038

Cited by 26 publications

(14 citation statements)

References 46 publications

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“…There have been efforts to integrate the risk assessment of SLOS as a part of screening for Down’s syndrome in the second trimester of pregnancy [50,51]. Interestingly, Anagnostopoulos et al have demonstrated decrease in apolipoprotein A-I and retinol binding protein in the second trimester amniotic fluid samples obtained from cases of Klinefelter syndrome (karyotype: 47, XXY) [52], and a study by Wang et al has shown that amniotic fluid apolipoprotein A-I is decreased in trisomy 18 (Edwards syndrome) and trisomy 21 (Down syndrome), while glycodelin-A is increased in trisomy 18 cases [53]. All of these observations support a potential for diagnostic application of the CMP in genetic disorders.…”

Section: Discussionmentioning

confidence: 99%

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Lee

Hassan

Romero

et al. 2011

Journal of Proteomics

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The cervical mucus plug (CMP) differs from the cervical secretions of non-pregnant women, and is the ultimate sealant of the uterine cavity during pregnancy. Although several studies have analyzed biochemical properties of large glycoproteins in the CMP, comprehensive information about its protein composition is yet unavailable. We hypothesized that protein profiling of the CMP could provide key clues to its physiological functions in pregnancy. For this purpose, five CMPs obtained from women in labor at term were analyzed by LC-MS/MS. Out of 291 total proteins identified, 137 were detected in two or more samples, which included S100A8, S100A9, and complement proteins (C3, C4a, C4b, C6, C8g). Several proteins, which have not been described in the cervical mucus of non-pregnant women or in cervicovaginal fluids, such as CD81 antigen and pregnancy zone protein, were also identified. Gene ontology analysis of identified proteins showed significant enrichment of 28 biological processes such as ‘activation of plasma proteins involved in acute inflammatory response’ and ‘positive regulation of cholesterol esterification’. We report the proteome of CMPs from pregnant women at term for the first time, and the overall findings strongly suggest an important role for the CMP in the maintenance of pregnancy and parturition.

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Section: Discussionmentioning

confidence: 99%

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Lee

Hassan

Romero

et al. 2011

Journal of Proteomics

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“…Procedures of networks analysis were similar to what we previously reported [33]–[35]. Briefly, we used the “analyze networks” algorithm in MetaCore (GeneGo, St. Joseph, MI) to build the networks that consisted of FSHR, HSD17B3 and CYP19.…”

Section: Methodsmentioning

confidence: 99%

Functional Analyses of Endometriosis-Related Polymorphisms in the Estrogen Synthesis and Metabolism-Related Genes

Wang

Cheng

et al. 2012

PLoS ONE

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Endometriosis is determined by genetic factors, and the prevalence of genetic polymorphisms varies greatly depending on the ethnic group studied. The objective of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) of 9 genes involved in estrogen biosynthesis and metabolism and the risks of endometriosis. Three hundred patients with endometriosis and 337 non-endometriotic controls were recruited. Thirty four non-synonymous SNPs, which change amino acid residues, were analyzed using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). The functions of SNP-resulted amino acid changes were analyzed using multiple web-accessible databases and phosphorylation predicting algorithms. Among the 34 NCBI-listed SNPs, 22 did not exhibit polymorphism in this study of more than 600 Taiwanese Chinese women. However, homozygous and heterozygous mutants of 4 SNPs - rs6165 (genotype GG+GA, 307Ala/Ala+307Ala/Thr) of FSHR, rs 6166 (genotype GG+GA, 680Ser/Asn+680Ser/Ser) of FSHR, rs2066479 (genotype AA+AG, 289Ser/Ser+289Ser/Gly) of HSD17B3 and rs700519 (genotype TT+TC, 264Cys/Cys+264Cys/Arg) of CYP19, alone or in combination, were significantly associated with decreased risks of endometriosis. Bioinformatics results identified 307Thr of FSHR to be a site for O-linked glycosylation, 680Ser of FSHR a phosphorylated site by protein kinase B, and 289Ser of HSD17B3 a phosphorylated site by protein kinase B or ribosomal protein S6 kinase 1. Results of this study suggest that non-synonymous polymorphisms of FSHR, HSD17B3 and CYP19 genes may modulate the risk of endometriosis in Taiwanese Chinese women. Identification of the endometrosis-preferential non-synonymous SNPs and the conformational changes in those proteins may pave the way for the development of more disease-specific drugs.

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“…As a carrier protein, TTR transports multiple molecules, including THs and retinol. Elevated levels of TTR have been reported in the amniotic fluid of fetuses with abnormal karyotypes, including trisomy 21 and trisomy 18 [8]. In serum, TTR levels decrease after 12 weeks of gestation [9], similar to the changing demands of TH of fetus.…”

Section: Introductionmentioning

confidence: 95%

Preeclampsia is associated with low placental transthyretin levels

Zhu

Baczyk

Lye

et al. 2016

Taiwanese Journal of Obstetrics and Gynecology

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The immunohistochemical expression of TTR in the syncytiotrophoblast layer of the placenta decreased significantly after 12 weeks of gestation, paralleling the changing demands of thyroid hormone uptake into the placenta. The reduced TTR expression in the syncytiotrophoblast layer of the preeclamptic placenta might impair thyroid hormone uptake and contribute to the pathophysiology of the disease.

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Network analyses of differentially expressed proteins in amniotic fluid supernatant associated with abnormal human karyotypes

Cited by 26 publications

References 46 publications

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Protein profiling underscores immunological functions of uterine cervical mucus plug in human pregnancy

Functional Analyses of Endometriosis-Related Polymorphisms in the Estrogen Synthesis and Metabolism-Related Genes

Preeclampsia is associated with low placental transthyretin levels

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