NET gains and losses: the role of changing nuclear envelope proteomes in genome regulation

Wong, Xianrong; Luperchio, Teresa Romeo; Reddy, Karen L.

doi:10.1016/j.ceb.2014.04.005

Cited by 57 publications

(57 citation statements)

References 156 publications

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“…The number of nuclei with damaged membranes and the percentage of DNA strand breaks were proportional to the applied UVC dose. The nuclear periphery (nuclear envelope and the underlying nuclear lamina) not only provides mechanical integrity but also triggers signaling cascades shaping nuclear architecture and gene regulation (Wong et al, 2014). Cell and nuclear membranes in human cell lines are targets vulnerable to UV radiation and the activation of membrane-bound cell death receptors is related to the process of apoptosis (Kulms and Schwarz, 2002).…”

Section: Discussionmentioning

confidence: 99%

A comparative analysis of membrane intactness and genome integrity in pea,barley, and wheat in response to UVC irradiation

Georgieva¹,

Nikolova²,

Bonchev³

et al. 2015

Turk J Bot

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The maintenance of plant genome integrity plays a critical function in the processes of DNA replication, transcription, and repair. Short-wave UV radiation (UVC) is among the most harmful agents known to affect genome stability and to induce DNA damage, including double-strand breaks (DSBs). Most previous studies in plants addressed the effects of UVC radiation at the physiological level; however, little research effort has been put into genome sensitivity across different plant species. Here, we made use of the trypan blue exclusion test and neutral comet assay to assess nuclear membrane and genome integrity in response to UVC radiation in monocot and dicot plants. We found that UVC radiation substantially affects nuclear membranes and the level of DSBs in a dose-responsive manner. Furthermore, differential sensitivity across plant species was observed, with monocot plants being less vulnerable to DSBs. This allows us to speculate that plant species with larger genomes may better tolerate UVC radiation.

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Section: Discussionmentioning

confidence: 99%

A comparative analysis of membrane intactness and genome integrity in pea,barley, and wheat in response to UVC irradiation

Georgieva¹,

Nikolova²,

Bonchev³

et al. 2015

Turk J Bot

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“…Lamins and widely expressed NETs such as LAP2β, LBR and emerin interact with transcriptionally silent chromatin [3,4,66]. For example, LBR binds heterochromatin protein 1 [67].…”

Section: The Two Faces Of the Ne — Tissue-specific Impacts On Chromatmentioning

confidence: 99%

“…There are now spectacular examples of tissue-specific 3D spatial organization of specific chromosomes and individual genes in the nucleus (reviewed in [3,4,80]). For example, in myoblasts the MyoD locus is maintained near the NE in a zone inaccessible to the TAF3 subunit of the TFIID activating complex; during myotube differentiation the MyoD locus moves to the nuclear interior where it can be activated [81].…”

Section: The Two Faces Of the Ne — Tissue-specific Impacts On Chromatmentioning

confidence: 99%

“…In animals these proteins include lamins, which form nuclear intermediate filaments, and growing numbers of NE transmembrane proteins (NETs). Many NETs, such as LEM-domain proteins (see Barton et al, this issue), localize specifically at the inner nuclear membrane (INM) and influence chromatin [3,4]. Other INM NETs, such as SUN-domain proteins, bind within the NE lumen to NETs known as Nesprins in the outer nuclear membrane (ONM), together forming complexes that Link the Nucleoskeleton and Cytoskeleton (LINC) [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Nuclear membrane diversity: underlying tissue-specific pathologies in disease?

Worman

Schirmer

2015

Current Opinion in Cell Biology

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Human ‘laminopathy’ diseases result from mutations in genes encoding nuclear lamins or nuclear envelope (NE) transmembrane proteins (NETs). These diseases present a seeming paradox: the mutated proteins are widely expressed yet pathology is limited to specific tissues. New findings suggest tissue-specific pathologies arise because these widely expressed proteins act in various complexes that include tissue-specific components. Diverse mechanisms to achieve NE tissue-specificity include tissue-specific regulation of the expression, mRNA splicing, signaling, NE-localization and interactions of potentially hundreds of tissue-specific NETs. New findings suggest these NETs underlie tissue-specific NE roles in cytoskeletal mechanics, cell-cycle regulation, signaling, gene expression and genome organization. This view of the NE as ‘specialized’ in each cell type is important to understand the tissue-specific pathology of NE-linked diseases.

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“…It will be interesting to learn how BAF 'shields' are dismantled, and how BAF contributes to chromosome re-engagement with lamins and nuclear-membrane proteins 6,14 , because these molecular connections are essential for customizing 3D genome organization in specific tissues and organs 15 . Further research might move beyond cell division, perhaps investigating the possibility of targeting BAF for anticancer therapies 16,17 , or detailing the mechanisms whereby BAF influences genome integrity, gene regulation and virus infection.…”

Section: News and Viewsmentioning

confidence: 99%