Nested Polymerase Chain Reaction for Mycobacterium tuberculosis DNA Detection in Aqueous and Vitreous of Patients with Uveitis

Ortega-Larrocea, Gabriela; Bobadilla-del-Valle, Miriam; Ponce-de-León, Alfredo; Sifuentes–Osornio, José

doi:10.1016/s0188-4409(02)00467-8

Cited by 59 publications

(31 citation statements)

References 20 publications

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“…MTb-PCR and has been used for early diagnosis of intraocular TB. [11][12][13] In one study, MTb-PCR was detected from aqueous specimens in 60% of patients with presumed intraocular TB. 11 PCR testing has yet to be standardized and is not recommended for TB diagnosis in the United Kingdom, because of its poor predictive value due to false negative results.…”

Section: Discussionmentioning

confidence: 99%

Clinical presentation, treatment, and outcomes in presumed intraocular tuberculosis: experience from Newcastle upon Tyne, UK

Manousaridis

Ong

Stenton

et al. 2013

Eye

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Purpose To report the clinical manifestations and treatment outcomes of patients with presumed intraocular tuberculosis (TB) seen at the Newcastle Uveitis Service, Royal Victoria Infirmary, Newcastle upon Tyne, UK over a 10-year period. Methods Retrospective review of case notes. Results A total of 21 patients were identified. Occlusive retinal vasculitis was the commonest ophthalmological presentation (12 patients). Eight patients (38%) were found to have underlying active systemic TB (four with mediastinal lymphadenopathy, three with pulmonary TB, one with cutaneous TB). Constitutional or respiratory symptoms, elevated inflammatory markers, and an abnormal chest radiograph were poor indicators of active TB. Two patients had inactive intrathoracic TB. Eleven patients had latent TB. Eighteen patients received anti-tuberculous treatment. Final visual acuity was better than or equal to initial visual acuity in 14 out of 16 patients who completed at least 6 months of standard anti-tuberculous treatment. Conclusions Most patients with presumed intraocular TB have latent TB, but a significant minority has hitherto undetected active TB. Our series suggests that either proven or presumed intraocular TB occurs frequently in the absence of constitutional or respiratory symptoms, elevated inflammatory markers, or an abnormal chest radiograph. A minimum of 6 months standard anti-tuberculous treatment provides good visual outcomes in the majority of patients.

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Section: Discussionmentioning

confidence: 99%

Clinical presentation, treatment, and outcomes in presumed intraocular tuberculosis: experience from Newcastle upon Tyne, UK

Manousaridis

Ong

Stenton

et al. 2013

Eye

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“…The QuantiFERON-Gold has demonstrated a specificity of 98.1% among 216 BCG-vaccinated Japanese nursing students who were entering their training and considered at low risk for 9 Polymerase chain reaction (PCR) is a sensitive and highly specific technique that has been employed to investigate presumed ocular tuberculosis with interesting results since the last decade. Gupta et al 10 13 Even though the use of nested PCR for M. tuberculosis may substantially improve sensitivity, major concerns still exist with regard to false positivity.…”

Section: Discussionmentioning

confidence: 99%

Combined Optic Neuropathy and Central Retinal Artery Occlusion in Presumed Ocular Tuberculosis without Detectable Systemic Infection

Ooi

Tai

Subrayan

et al. 2011

Ocular Immunology and Inflammation

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“…Eales hastal›¤› olan hastalar›n epiretinal membran örneklerinin %47,8'inde TBC nPCR ile pozitif saptan›rken olas› intraoküler TBC olan hastalar›n %77'sinde TBC pozitif bulunmufltur. 12,13 Gerçek zamanl› PCR kontaminasyon ile enfeksiyona yol açan patojenin ay›rt edilebilece¤i bir PCR tekni¤i-dir.…”

Section: Nükleik Asit Amplifikasyon Analizleriunclassified

Oküler Tüberküloz II: Tanı ve Tedavi

Onal¹,

Tutkun²

2011

Tjo

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ÖzetSon dönemlerde tüberkülin deri testi (TDT), interferon gama sal›n›m analizleri (IGRA), toraks›n bilgisayarl› tomografisi ve polimeraz zincir reaksiyonu ile ilgili yap›lm›fl çal›flmalar oküler tüberküloz (TBC) tan›s›n›n konulmas›nda yeni yaklafl›mlar›n do¤mas›na neden olmufltur. Ancak, TDT ve IGRA latent TBC enfeksiyonunun ortaya konmas›nda yard›mc›d›r ve aktif enfeksiyonu ay›rt ettirmez. ‹ntraoküler TBC tan›s›n› destekleyici di¤er bir test ise akci¤erin grafi veya bilgisayarl› tomografi yolu ile görüntülemesidir. Tan› SummaryRecent studies on the clinical importance of tuberculin skin tests (TST), interferon-gamma release assays (IGRAs), chest computed tomography (CT) and polymerase chain reaction have provided a new approach to diagnosing ocular tuberculosis (TBC). However, both TST and IGRAs cannot distinguish between latent TBC infection and active disease. Another corroborative test in the diagnosis of intraocular TBC is the evaluation of the lungs by either chest radiography or CT. Direct evidence for the diagnosis can be obtained by examination of smears and staining for acid-fast organisms, cultures of intraocular tissue/fluid for Mycobacterium tuberculosis, and nucleic acid amplification analysis. In recent years, guidelines have been suggested for the diagnosis of intraocular TBC. The current treatment of intraocular TBC consists of use of four drugs (isoniazid, rifampicin, ethambutol and pyrazinamide) taken for a long period of time (total 9-15 months). (Turk J Ophthalmol 2011; 41: 182-90)

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Nested Polymerase Chain Reaction for Mycobacterium tuberculosis DNA Detection in Aqueous and Vitreous of Patients with Uveitis

Cited by 59 publications

References 20 publications

Clinical presentation, treatment, and outcomes in presumed intraocular tuberculosis: experience from Newcastle upon Tyne, UK

Clinical presentation, treatment, and outcomes in presumed intraocular tuberculosis: experience from Newcastle upon Tyne, UK

Combined Optic Neuropathy and Central Retinal Artery Occlusion in Presumed Ocular Tuberculosis without Detectable Systemic Infection

Oküler Tüberküloz II: Tanı ve Tedavi

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