Nerve structures of the heart and their immunohistochemical characterization in 10-week-old porcine foetuses

Franke-Radowiecka, Amelia; Zmijewska, Nina; Zubkiewicz, Tomasz; Załęcki, Michał; Klimczuk, Magdalena; Listowska, Żaneta; Kaleczyc, Jerzy

doi:10.5802/crbiol.4

Cited by 3 publications

(4 citation statements)

References 30 publications

(79 reference statements)

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“…No CGRP‐positive ganglionic cell bodies were identified in our whole‐mount preparations of the piglet atria. This is in sharp contrast to study by Franke‐Radowiecka et al (2020) which reports about CGRP‐positive neuronal somata observed in the hearts of porcine fetuses. Temporary expression of this neuropeptide during prenatal development may explain these differences.…”

Section: Discussioncontrasting

confidence: 99%

“…The revealed abundance of adrenergic NS is in line with the quantity of these cells in the piglet cardiac ventricles (Pauziene et al, 2017). Interestingly, adrenergic NS in the heart of 10‐week‐old porcine fetus composed only about 1.5% of all intrinsic cardiac somata revealed in the study by Franke‐Radowiecka et al (2020), as well as these neural cells were scarce in the heart conductive system of young adult pigs aged 3–6 months (Crick et al, 1999). Thereby, it may be implied that adrenergic as well as cholinergic NS develop throughout the prenatal and the early postnatal periods.…”

Section: Discussionmentioning

confidence: 99%

“…By now, only the quantitative age‐related changes in the number of ICNs were examined providing quite contradictory data (Batulevicius et al, 2003, 2005; Pauza et al, 2000, 2002; Saburkina et al, 2009). Earlier heart neuroanatomical studies of both the fetal and the newborn pig ( Sus scrofa domesticus Erxleben, 1777) were lacking a quantitative evaluation of the abundance of diverse neuronal phenotypes within cardiac GP (Crick et al, 1999; Franke‐Radowiecka et al, 2020). Therefore, the present study aims to identify the relationship between chemical phenotypes of ICNs and their location in the atrial GP of the newborn pig prospecting for a comparative analysis of cardiac GP in the adult pig that is the most popular animal model in experimental cardiothoracic surgery and heart electrophysiology due to its anatomical similarity with the human heart, and its convenience for handling in regard to dimensions and weight (Garg et al, 2013; Suzuki et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777)

Ragauskas

Rysevaitė-Kyguolienė

Paužienė

et al. 2021

Journal of Morphology

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Intrinsic cardiac neurons (ICNs) are crucial cells in the neural regulation of heart rhythm, myocardial contractility, and coronary blood flow. ICNs exhibit diversity in their morphology and neurotransmitters that probably are age‐dependent. Therefore, neuroanatomical heart studies have been currently focused on the identification of chemical phenotypes of ICNs to disclose their possible functions in heart neural regulation. Employing whole‐mount immunohistochemistry, we examined ICNs from atria of the newborn pigs (Sus scrofa domesticus) as ICNs at this stage of development have never been neurochemically characterized so far. We found that the majority of the examined ICNs (>60%) were of cholinergic phenotype. Biphenotypic neuronal somata (NS), that is, simultaneously positive for two neuronal markers, were also rather common and distributed evenly within the sampled ganglia. Simultaneous positivity for cholinergic and adrenergic neuromarkers was specific in 16.4%, for cholinergic and nitrergic—in 3.5% of the examined NS. Purely either adrenergic or nitrergic ICNs were observed at 13% and 3.1%, correspondingly. Purely adrenergic and nitrergic NS were the most frequent in the ventral left atrial subplexus. Similarly to neuronal phenotype, sizes of NS also varied depending on the atrial region providing insights into their functional implications. Axons, but not NS, positive for classic sensory neuronal markers (vesicular glutamate transporter 2 and calcitonin gene‐related peptide) were identified within epicardiac nerves and ganglia. Moreover, a substantial number of ICNs could not be attributed to any phenotype as they were not immunoreactive for antisera used in this study. Numerous dendrites with putative peptidergic and adrenergic contacts on cholinergic NS contributed to neuropil of ganglia. Our observations demonstrate that intrinsic cardiac ganglionated plexus is not fully developed in the newborn pig despite of dense network of neuronal processes and numerous signs of neural contacts within ganglia.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777)

Ragauskas

Rysevaitė-Kyguolienė

Paužienė

et al. 2021

Journal of Morphology

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“…In this experiment, there was no presence of nerve fibers containing CART and/or CGRP, although previous studies have described both of these substances in the intracardiac nervous structures [63][64][65][66]. The absence of CGRP positive nerves is especially surprising because this substance in the light of previous studies is known as an important factor participating, among others, in the regulation of the blood flow in vessels located in the heart wall [67], showing protective effects on cardiomyocytes [67,68] and increasing the heart rate [69].…”

Section: Discussionmentioning

confidence: 49%

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Makowska

Gonkowski

2021

Animals

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Bisphenol A (BPA) contained in plastics used in the production of various everyday objects may leach from these items and contaminate food, water and air. As an endocrine disruptor, BPA negatively affects many internal organs and systems. Exposure to BPA also contributes to heart and cardiovascular system dysfunction, but many aspects connected with this activity remain unknown. Therefore, this study aimed to investigate the impact of BPA in a dose of 0.05 mg/kg body weight/day (in many countries such a dose is regarded as a tolerable daily intake–TDI dose of BPA–completely safe for living organisms) on the neurochemical characterization of nerves located in the heart wall using the immunofluorescence technique. The obtained results indicate that BPA (even in such a relatively low dose) increases the number of nerves immunoreactive to neuropeptide Y, substance P and tyrosine hydroxylase (used here as a marker of sympathetic innervation). However, BPA did not change the number of nerves immunoreactive to vesicular acetylcholine transporter (used here as a marker of cholinergic structures). These observations suggest that changes in the heart innervation may be at the root of BPA-induced circulatory disturbances, as well as arrhythmogenic and/or proinflammatory effects of this endocrine disruptor. Moreover, changes in the neurochemical characterization of nerves in the heart wall may be the first sign of exposure to BPA.

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Intrinsic cardiac neurons of the adult pigs: chemical types, abundance, parameters and distribution within ganglionated plexus

Ragauskas¹,

Rysevaitė-Kyguolienė²,

Pauziene³

et al. 2022

Annals of Anatomy - Anatomischer Anzeiger

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Nerve structures of the heart and their immunohistochemical characterization in 10-week-old porcine foetuses

Abstract: Nerve structures of the heart and their immunohistochemical characterization in 10-week-old porcine foetuses

Cited by 3 publications

References 30 publications

Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777)

Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777)

Changes Caused by Low Doses of Bisphenol A (BPA) in the Neuro-Chemistry of Nerves Located in the Porcine Heart

Intrinsic cardiac neurons of the adult pigs: chemical types, abundance, parameters and distribution within ganglionated plexus

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