Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Sciatic Nerve Regeneration in Mice Using A PLGA Microgroove Patterned Conduit Fills with Microfiber

“…The fibrous filler is took inspiration from the observation of peripheral nerve system ECM, in vivo, which facilitates neurite and cellular migration. According to our previous study, the migration rate of nerve cell cultured on poly(lactic-co-glycolic acid) (PLGA) microfiber is 1.6-fold comparing with flat PLGA membrane [4]. And, the neurite extenuation shows identical direction with microfiber.…”

“…It has been well know that the micro pattern directed cell migration and alignment by restriction. 10-30 m in width of pattern has been proved that is benefice on cell guidance and improving neurite elongation [4][5][6][7].…”

“…In view of fibrous filler and micro-patterns are beneficial to cell migration and neurite orientation, we develop a hybrid nerve conduit which is microgroove patterned conduit filling with a bunch of microfiber to estimate in vitro functionality [4]. 90% of the cells in the hybrid conduit grow in the direction of the designed patterns and microfiber.…”

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

“…They are usually applied to smaller nerves and overcome the disadvantages of the organic options [45]. To ensure its functionality, the characteristics of the NGCs used must comply with all the criteria established for this type of biomaterials: the material used must be (i) biocompatible with the regenerating tissue where it will be applied and should never trigger any local or systemic inlammatory response [46]; (ii) biodegradable, while ensuring mechanical and architectural stability during the regenerative process and resisting to the application of sutures and to inlammatory tissue reaction [47]; (iii) lexible and resistant in a balanced way in order to avoid compression of the regenerating axons and to limit tissue inlammation [48]; (iv) capable of preventing the growth of excessive ibrous tissue associated with the site of injury and reducing the loss of neurotrophic factors secreted by damaged nerve endings [44]; (v) capable to provide an orientation line to the growth cone through a 3-D tubular structure, thereby diminishing misdirection phenomena [49]; (vi) semi-permeable and with pores of adequate diameter that allow the inlux of oxygen and interstitial nutrients to nourish the growing axon that simultaneously prevent the entry of inlammatory cells and the loss of growth factors [50]; technically eicient, ensuring requirements related to production, sterilization, storage and handling [51]. Adapted in each case, these biomaterials must have appropriate dimensions that allow the connection of the nerve defects without tension, and the diameter of the conduit and the thickness of the wall should be suicient to accommodate the two stumps at the nerve ends without any compression being exerted.…”

Untitled

“…Some 3D grafts for nerve conduits were developed and successfully tested both in-vitro and in-vivo. Gelatin-based nanoporous, 60 silk fibroin/silk sericin, 61 water-based biodegradable polyurethane, PLGA, PLA/collagen, PCL, 62 with Mesenhimal Stem Cells (MSCs), neural stem/progenitor cells (KT98/ F1B-GFP) and Schwann cell have shown promising results during long term in-vivo studies. …”