Nerve growth factor enhances regeneration through silicone chambers

Rich, Keith M.; Alexander, Todd D.; Pryor, Johnny C.; Hollowell, James P.

doi:10.1016/0014-4886(89)90115-5

Cited by 215 publications

(107 citation statements)

References 32 publications

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“…The average diameter of the fibers depended on the movement speed of the substrate. The higher movement growth factors play important roles in promoting and directing process outgrowth and elongation (1,4,11,12,24). rate is the smaller the diameter of fibers.…”

Section: Explants Of Peripheral Nerve Cultures Synthesis Of Styrenatementioning

confidence: 99%

“…Recent studies have shown over the years have been performed to develop neurosurgical prostheses using a tubular conduit or graft to that these bioresorbable conduits exhibit excellent biocompatibility and controlled biodegradability (1-3,6,8-10, accelerate nerve repair in the peripheral nervous system (PNS). The conduit was implanted empty or filled with [14][15][16][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. In addition to the use of a tubular conduit as a scaffold, two other materials have been incorporated adhesive proteins, growth factors, cells, or guidance fibers.…”

Section: Introductionmentioning

confidence: 99%

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Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold

et al. 2003

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We devised a novel nerve prosthesis composed of an elastomeric gelatinous tube and multifilament gelatinous fibers, both of which were prepared from styrene-derivatized gelatin, which allows in situ formation of a bioactive substance-incorporated gel. An in vitro study showed that the axonal regeneration potential of a photocured gelatin layer impregnated with laminin, fibronectin, and NGF was almost comparable with that of coated Matrigel. A nerve conduit and fibers prepared from photoreactive gelatin was subjected to visiblelight irradiation with rotation in the presence of camphorquinone as a photoinitiator using a custom-designed apparatus. A sample of transparent gelatinous conduit with an inner diameter of 1.2 mm and a wall thickness of 0.6 mm and gelatin fibers ranging from 10 to 100 µm in diameter were produced. The photocured elastomeric gelatinous tube was flexible and had structural integrity that allowed mechanical handling without breaking. A novel nerve guidance prosthesis composed of tubes packed with fibers was assembled. This photofabrication technology may enable the design of a tailor-made shape and rapid morphogenesis and functional recovery of damaged nerve tissue.

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Section: Explants Of Peripheral Nerve Cultures Synthesis Of Styrenatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold

et al. 2003

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“…After nerve section there is an increase in NGF-like activity in the distal segment of the nerve (Richardson & Ebendal, 1982) and Schwann cells show an increase in both mRNA for NGF (Bandtlow, Heumann, Schwab & Thoenen, 1987) and the low-affinity NGF receptors (Taniuchi, Clark & Johnson, 1986). Administration of NGF to an injury site enhances the regeneration of myelinated axons (Rich, Alexander, Pryor & Hollowell, 1989) and NGF has been shown to increase the expression of a cell adhesion molecule on the Schwann cells which may influence the growth of regenerating neurites (Seilheimer & Schachner, 1987).…”

Section: Discussionmentioning

confidence: 99%

Nerve growth factor depletion reduces collateral sprouting of cutaneous mechanoreceptive and tooth‐pulp axons in ferrets.

Doubleday¹,

Robinson²

1994

The Journal of Physiology

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1. Electrophysiological experiments were carried out to determine whether or not collateral sprouting of cutaneous low-threshold mechanoreceptive fibres could be detected and to investigate the effect of nerve growth factor (NGF) deprivation on the sprouting of these fibres and the fibres innervating tooth pulps. 2. In twenty-one ferrets (eleven of which had been autoimmunized against NGF) the right inferior alveolar nerve (IAN) was sectioned and prevented from regenerating. After 12 weeks, transmedian innervation from the left IAN was determined by stimulating the nerve whilst recording from electrodes implanted in the contralateral anterior teeth and also by single unit recordings from the nerve whilst mechanically and electrically stimulating the skin. The results were compared with those from ten control animals. 3. Transmedian innervation of contralateral teeth was found in none of the control animals; in all ten of the animals which had undergone denervation without immunization (4/10 canines, 17/20 incisors); but in only six of the eleven immunized and denervated animals (0/11 canines, 7/22 incisors). 4. Of 270 cutaneous mechanoreceptive units sampled in the controls, only four units had transmedian receptive fields, extending a maximum of 1 mm across the mid-line. After denervation, significantly more units (42 of 274) crossed the mid-line and extended up to 4 mm. After immunization and denervation only eleven of 305 units crossed the midline by a maximum of t mm.5. These data show that cutaneous low-threshold mechanoreceptive Af8 and Ad fibres, as well as Ad tooth pulp fibres, are able to undergo collateral sprouting. This sprouting is partially blocked by NGF depletion, suggesting that NGF plays an essential role in the process.

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“…Insulin [199,200], and insulin-like growth factor-1 [220][221][222][223][224] within a bridging tube significantly increase the number of Schwann cells that migrate into the tube. Additional factors that induce Schwann cell proliferation are platelet-derived growth factor-B (PDGF-B), acidic and basic fibroblast growth factors (b-FGF and a-FGF) [225], transforming growth factor (TGF-β) [226,227] and neuregulins [224,228,229]. Axolemmal membrane also stimulates proliferation of cultured Schwann cells [230][231][232][233][234], while NGF added to a tube enhances axon ingrowth [235][236][237].…”

Section: Addition Of Neurotrophic Factors Cytokines and Other Factomentioning

confidence: 99%

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Kuffler¹

2015

Int J Neurorehabilitation Eng

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Repairing Peripheral Nerves following Traumas Nerve crushWithin several days of crushing a peripheral nerve, the injured axons begin to regenerate through their distal pathway until they reach their original targets with which they restore neurological function. The larger the number of axons that regenerate through the distal nerve, the greater the extent of neurological recovery [1][2][3]. The number of axons that regenerate, and thus neurological recovery, is influenced by the physiological state of the distal denervated nerve pathway [4][5][6][7].Following a peripheral nerve crush, the injured axons begin to regenerate within 2 days of injury [8]. They may regenerate to their targets through their original pathway in association with and promoted by its Schwann cells, the neurotrophic factors they release, and the Schwann cell extracellular matrix [1,4,[9][10][11][12][13][14]. Thus, the distal nerve provides a simple means for promoting and directing the regenerating axons to their original targets [15][16][17][18]. However, when a nerve has a gap, the gap must be bridged with a material that is permissive to and promotes axon regeneration across the entire gap to reach the distal nerve stump, through which the axons must then be able to regenerate.The Schwann cells in the denervated distal nerve release neurotrophic factors that promote axon regeneration, but also release extracellular matrix components that promote and inhibit axon regeneration [19][20][21][22][23][24][25][26]. Thus, regeneration through the distal nerve is a balance of the influences of factors that both promote and inhibit regeneration. If the Schwann cells are present, such as after a simple nerve crush, virtually 100% of the axons regenerate and they innervate all the denervated synaptic sites. If the Schwann cells within the distal nerve pathway are killed, leaving only the extracellular matrix intact, the number of axons that regenerates to their targets decreases by 94%. This is because the factors required to trigger the regenerating axons to branch at extracellular matrix branch points are missing, axons do not branch, and therefore each axon reinnervates only a single denervated muscle fiber. Thus, Schwann cells along the distal nerve pathway and a AbstractFollowing a peripheral nerve crush, or when a peripheral the nerve is transected and the nerve stumps anastomosed, neurological recovery is generally excellent. This is because the axons merely have to regenerate through the distal portion of the nerve through the existing extracellular matrix of the denervated Schwann cells that direct and promote them to their distant denervated motor and sensory targets. However, when a length of a peripheral nerve is destroyed, and anastomosis is not possible, the standard surgical repair technique is to graft a length/s of autologous sensory nerve into the gap. Neurological recovery is generally good if the nerve gap is <2 cm in length, the repair is performed <4 months post trauma, and the patients are <25 years of age. Because the nerve ...

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Nerve growth factor enhances regeneration through silicone chambers

Cited by 215 publications

References 32 publications

Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold

Photoconstructs of Nerve Guidance Prosthesis Using Photoreactive Gelatin as a Scaffold

Nerve growth factor depletion reduces collateral sprouting of cutaneous mechanoreceptive and tooth‐pulp axons in ferrets.

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

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