Nerve conduit filled with GDNF gene-modified schwann cells enhances regeneration of the peripheral nerve

Li, Qingfeng; Ping, Ping; Jiang, Hao; Liu, Kai

doi:10.1002/micr.20192

Cited by 92 publications

(68 citation statements)

References 18 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There is evidence indicating that nerve grafting is essential for reconstruction of long nerve defects. Recently, neural tissue engineering has received much attention, and Schwann cell transplantation has been reported to achieve reliable outcomes in the regeneration of the sciatic nerve (1)(2)(3). However, Schwann cell isolation can cause additional damage, and these cells need a long time for cell culture and growth, which have limited their clinical application.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of adipose-derived stem cells for peripheral nerve repair

Liu

Cheng²,

Guo³

et al. 2011

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Mesenchymal stem cells have become a very attractive source of cell implantation for neural tissue engineering. The ideal stem cells for transplantation should be easily obtained, and should rapidly proliferate in vitro and have low immunogenicity. The purpose of this study was to investigate the regenerative potential of adipose-derived stem cells (ADSC) on peripheral nerve repair. ADSCs were isolated from rat adipose tissue and cultured until adherent cells became morphologically homogeneous with a fibroblast-like shape, and transplanted with acellular nerve allografts (ANAs) into rat models with a 10 mm gap of transected sciatic nerve defect. After cell transplantation, we found that ADSC implantation improved functional recovery of exercise behavior and increased wet weight ratio of the anterior tibial muscle. In the electrophysiological testing, we found that the percentage of activated fibers was higher in the ADSC-implanted animals as evidenced by the increase of nerve conduction velocity and amplitude. Histological examination revealed that the number of nerve fibers, axonal diameter and myelin thickness were significantly higher in the ADSC-implanted animals compared to the control. In addition, we demonstrated that the progression of the regenerative process after ADSC implantation was accompanied by elevated expression of neurotrophic factors at both the early and later phase. Taken together, these results suggest that ADSCs can promote the repair of peripheral nerve injury, and the combination of ADSC and ANA transplantation is a new therapeutic method for long distant peripheral nerve defects. Our data also provide evidence indicating the strong association of neurotrophic factor production to the regenerative potential of implanted ADSCs. IntroductionTraditional therapeutic approaches for the reconstruction of peripheral nerve defects include end-to-end suturing, fascicular suturing, nerve graft, and nerve conduits. There is evidence indicating that nerve grafting is essential for reconstruction of long nerve defects. Recently, neural tissue engineering has received much attention, and Schwann cell transplantation has been reported to achieve reliable outcomes in the regeneration of the sciatic nerve (1-3). However, Schwann cell isolation can cause additional damage, and these cells need a long time for cell culture and growth, which have limited their clinical application.Mesenchymal stem cells (MSC) have recently become a very attractive source of cell implantation for tissue engineering because of their self-proliferation, fast proliferation and multilineage differentiation potential (4). A large number of studies have shown that bone marrow-derived mesenchymal stem cells (BMMSC) can promote the repair of peripheral nervous system injury (5-11). Chen et al (11) found that rat BMMSC can synthesize and secrete a number of neurotrophic factors (NF), including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factors (CNTF) and glial cell line-derive...

show abstract

Section: Introductionmentioning

confidence: 99%

Transplantation of adipose-derived stem cells for peripheral nerve repair

Liu

Cheng²,

Guo³

et al. 2011

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…First, given its nontoxicity and a wide margin of biologic safety, it may be used as an effective physical stimulant when engineering peripheral nerve tissue. Schwann cell-based therapies that use transplantation techniques for the treatment of nerve tissue repairing are being widely investigated for their www.intechopen.com potential as clinical applications (Rochkind, Astachov et al 2004;Li, Ping et al 2006;Gravvanis, Lavdas et al 2007). LIPUS applied in conjunction with other forms of biologic stimulation, is worth considering when optimizing an innovative "multi-level" form of treatment.…”

Section: Lipus Enhances Neurite Elongation In Rat Cortical Neuronsmentioning

confidence: 99%

Effect of Low-Intensity Pulsed Ultrasound on Nerve Repair

Li¹,

Zhang²,

Ren³

2012

Tissue Regeneration - From Basic Biology to Clinical Application

View full text Add to dashboard Cite

“…In fact, cultured Schwann cells from sciatic nerve has been employed in the spinal cord regeneration experimentally 12 , rising the necessity of a more detailed analysis on the presence of neurotrophic molecules in those cells in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

et al. 2008

View full text Add to dashboard Cite

PURPOSE: The neurotrophic factor fibroblast growth factor-2 (FGF-2, bFGF) and Ca++ binding protein S100ß are expressed by the Schwann cells of the peripheral nerves and by the satellite cells of the dorsal root ganglia (DRG). Recent studies have pointed out the importance of the molecules in the paracrine mechanisms related to neuronal maintenance and plasticity of lesioned motor and sensory peripheral neurons. Moreover, cultured Schwann cells have been employed experimentally in the treatment of central nervous system lesions, in special the spinal cord injury, a procedure that triggers an enhanced sensorymotor function. Those cells have been proposed to repair long gap nerve injury. METHODS: Here we used double labeling immunohistochemistry and Western blot to better characterize in vitro and in vivo the presence of the proteins in the Schwann cells and in the satellite cells of the DRG as well as their regulation in those cells after a crush of the rat sciatic nerve. RESULTS: FGF-2 and S100ß are present in the Schwann cells of the sciatic nerve and in the satellite cells of the DRG. S100ß positive satellite cells showed increased size of the axotomized DRG and possessed elevated amount of FGF-2 immunoreactivity. Reactive satellite cells with increased FGF-2 labeling formed a ring-like structure surrounding DRG neuronal cell bodies.Reactive S100ß positive Schwann cells of proximal stump of axotomized sciatic nerve also expressed higher amounts of FGF-2. CONCLUSION: Reactive peripheral glial cells synthesizing FGF-2 and S100ß may be important in wound repair and restorative events in the lesioned peripheral nerves.

show abstract

Nerve conduit filled with GDNF gene-modified schwann cells enhances regeneration of the peripheral nerve

Cited by 92 publications

References 18 publications

Transplantation of adipose-derived stem cells for peripheral nerve repair

Transplantation of adipose-derived stem cells for peripheral nerve repair

Effect of Low-Intensity Pulsed Ultrasound on Nerve Repair

S100ß and fibroblast growth factor-2 are present in cultured Schwann cells and may exert paracrine actions on the peripheral nerve injury

Contact Info

Product

Resources

About