A five weeks biological experiment was planned to examine the impacts of dietary supplementation with of Nano sized of zinc oxide (ZONPs) on productive performance, carcass traits, organ relative weights, serum biochemical parameters, histological alteration in some internal organs and concentration of this element in the serum, liver, thigh and breast muscle in broiler chicks. A total of 108 day old newly hatched unsexed commercial broiler chicks were individually weighed and equally distributed in a completely randomized design arrangement according to the dose of zinc oxide nanoparticles (ZONPs) supplementation into 3 dietary experimental groups, with 3 replications having 12 birds per replicate (n = 36/ treatment) with feed and water were provided ad libitum consumption through 5 weeks life span. The 3 Experiential dietary treatments (T1, T2 and T3) received corn-soybean meal-based diets enhanced with 0 (control), 40 and 60 mg / kg diet of ZONPs respectively. Present results has shown that after 5 weeks of feeding trial and as compared to control, the ZONPs supplementation groups recorded higher body weight, improved feed consumption, feed conversion ratio and performance index. Serum biochemical analyses revealed that serum cholesterol, triglyceride, low density lipoprotein and uric acid decreased significantly, while high density lipoprotein and liver enzyme (AST,ALT) concentrations were significantly increased in ZONPs groups than control. Meanwhile, zinc accumulation in serum, liver and breast and thigh muscle were linearly increased with increasing zinc supplementation. It could be concluded that supplementation of ZONPs to broiler diet at 40 or 60 mg/kg improved productive performance, birds' physiological status and the lower levels Zn (40 mg/kg diet) revealed promising results and can be used as an effective feed additive in broilers.