Nephroprotective effects of Zingiber zerumbet Smith ethyl acetate extract against paracetamol-induced nephrotoxicity and oxidative stress in rats

Abstract

Abstract:Paracetamol (PCM) overdose can cause nephrotoxicity with oxidative stress as one of the possible mechanisms mediating the event. In this study, the effects of ethyl acetate extract of Zingiber zerumbet rhizome [200 mg per kg of body weight (mg/kg) and 400 mg/kg] on PCM-induced nephrotoxicity were examined. Rats were divided into five groups containing 10 rats each. The control group received distilled water while other groups were treated with extract alone (400 mg/kg), PCM alone (750 mg/kg), 750 mg/kg PCM+200 mg/kg extract (PCM+ 200-extract), and 750 mg/kg PCM+400 mg/kg extract (PCM+400-extract), respectively, for seven consecutive days. The Z. zerumbet extract was given intraperitoneally concurrent with oral administration of PCM. Treatment with Z. zerumbet extract at doses of 200 and 400 mg/kg prevented the PCM-induced nephrotoxicity and oxidative impairments of the kidney, as evidenced by a significantly reduced (P<0.05) level of plasma creatinine, plasma and renal malondialdehyde (MDA), plasma protein carbonyl, and renal advanced oxidation protein product (AOPP). Furthermore, both doses were also able to induce a significant increment (P<0.05) of plasma and renal levels of glutathione (GSH) and plasma superoxide dismutase (SOD) activity. The nephroprotective effects of Z. zerumbet extract were confirmed by a reduced intensity of renal cellular damage, as evidenced by histological findings. Moreover, Z. zerumbet extract administered at 400 mg/kg was found to show greater protective effects than that at 200 mg/kg. In conclusion, ethyl acetate extract of Z. zerumbet rhizome has a protective role against PCM-induced nephrotoxicity and the process is probably mediated through its antioxidant properties.

The modulatory effect of Moringa oleifera leaf extract on endogenous antioxidant systems and inflammatory markers in an acetaminophen-induced nephrotoxic mice model

“…The renal histological results correlate well with the alterations noted in biochemical parameters. The APAP-intoxicated mice revealed severely disorganized glomerulus, dilated tubules, and inflammatory casting, which is in agreement with the previous studies ( Hamid et al , 2012 ; Ahmad et al , 2012 ), whereas MO leaf extract at a higher dose (200 mg/kg) preserved glomerulus and tubular architecture with insignificant tubular casting, similar to the positive control/sham group. However, the histological findings in APAP-intoxicated mice were at variance with that of ( Sharifudin et al , 2013 ), where male Sprague-Dawley rats administered 7 g/kg body weight of APAP did not reveal any significant changes in kidney histology.…”

Hydroethanolic Extract, Naringin and Naringenin in N-Acetyl-P-aminophenol-administered Wistar Rats

“…The decreased SOD and GSH levels and the increased LPO in the APAP-administered rats, in present study, were in accordance with previous research studying APAP-induced nephrotoxicity (Ghosh and Sil, 2007;Yousef et al, 2010;Abdul Hamid et al, 2012;Naguib et al, 2014;Hommore et al, 2015).…”

Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach

“…The flavonoids that play a role as antioxidants in biological systems are natural phenolic compounds present in fruit and vegetable species [14]. Flavonoids have many useful effects that include being antioxidants and having anti-bacterial, anti-cancer, anti-mutagenic and anti-inflammatory properties [15,16,17].…”