Néphrocalcinose expérimentale par injection de parathormone. Etude au microanalyseur à sonde électronique

Berry, Janice E.

doi:10.1159/000179813

Cited by 16 publications

(2 citation statements)

References 8 publications

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“…Second, the treatment of both types of aggregate with formic acid results in the complete removal of their granular and crystalline material, as discussed in detail below . Moreover, as far as the crystalline aggregates are concerned, their inorganic nature is proved by the fact that they are found in von Kossa-positive, alizarin red S-stained mitochondria and by the observation that they consist of structures which have the same morphology and give the same electron diffractograms as the inorganic crystals found in bone, cartilage, and dentine (8-10, 18, 19, 22, 23, 29, 30, 43, 46, 67, 117, 122, 132, 137) and in many calcified soft tissues (13,16,37,48,55,68,90,98,99,116) .…”

Section: Nature Of Intramitochondrial Aggregatesmentioning

confidence: 99%

The Organic-Inorganic Relationship in Calcified Mitochondria

Bonucci

Derenzini

Marinozzi

1973

The Journal of Cell Biology

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Experimentally induced calcification within mitochondria has been studied electron microscopically . Cells investigated comprise hepatic cells damaged by CC1 4 intoxication, myocardial cells damaged by prolonged dihydrotachysterol (DHT) administration, and cells from skeletal muscle (gastrocnemius) damaged by DHT sensibilization and local injury. Cells from a human bowel carcinoma were studied too . Two types of intramitochondrial inorganic inclusion have been found . The first consists of clusters of apatite-like, needle-shaped crystals (crystalline aggregates), the second of clusters of very fine granules (granular aggregates) . The former have been found mainly in mitochondria in apparently normal myocardial and muscular cells, the latter in mitochondria of degenerated hepatic, neoplastic, and myocardial cells . Crystalline aggregates are closely related to the membranes of cristae at first, but they later spread to occupy the whole mitochondrial matrix . Granular aggregates are initially found in the mitochondrial matrix near, but perhaps not touching, cristae ; by growing they come into close contact with cristal membranes . Both types of aggregate show intrinsic electron opacity, which disappears after formic acid decalcification . Only the crystalline aggregates give an electron diffraction pattern of crystallinity . Uranium and lead staining of decalcified sections shows that both types of aggregate are intimately connected with an organic substrate . The substrate of crystalline aggregates consists of very thin, elongated structures shaped like the inorganic crystals . The substrate of granular aggregates consists of amorphous material gathered in clusters, with the same roundish shape and intercristal position as the inorganic granules. Both types of substrate are stained by phosphotungstic acid at low pH and by silver nitrate-methenamine after periodic acid oxidation . These results show that the organic content of the substrates includes glycoproteins ; they have been confirmed by the periodic acid-Schiff (PAS) method under the optical microscope . These findings have been discussed in relation to the recent discovery of organic Cat+-binding sites in mitochondria and to the general problems of soft tissue calcification .

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Section: Nature Of Intramitochondrial Aggregatesmentioning

confidence: 99%

The Organic-Inorganic Relationship in Calcified Mitochondria

Bonucci

Derenzini

Marinozzi

1973

The Journal of Cell Biology

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“…Although the mean papillary calcium concentration was high, the mean concentration in medulla and cortex in these kidneys was proportionately much higher, with a reversed gradient. This is not surprising, since in many reported instances of both clinical and experimental hypercalcamia, calcification commonly occurs in the cortex and outer medulla (Allen, 1962;Berry, 1970). Gains ef al.…”

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An Investigation of Factors Affecting the Ditribution of Calcium in the Human Kidney1

Cooke

1971

British Journal of Urology

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A STUDY of the distribution of calcium in the human hdney has resulted in the demonstration of a calcium concentration gradient in the renal medulla, with high values in the papilla (Cooke, 1971). This distribution is present both in kidneys analysed immediately after nephrectomy and in specimens obtained at necropsy up to 76 hours after death. The size of the medullary calcium gradient, as measured in necropsy material, varies between individual kidneys, largely due to differences in the amount of calcium in the papilla. The significance of this variation in papillary calcium concentration is unknown, and the nature of calcium binding in the papilla obscure.Gains et al. (1968) have suggested that a calcium concentration gradient in the mammalian renal medulla may result from a physiological process similar to that by which sodium is concentrated in papillary tissue; Hargitay and Kuhn (1951) have explained the hyperosmolarity in the renal papilla in terms of reabsorption from the ascending limb in association with a countercurrent multiplier system involving the loops of Henle, sodium being the main ion involved (Gottschalk and Mylle, 1959).The relatively high concentration of calcium in the papilla is reflected, in the human kidney, in the frequent presence of calcification. The cause of papillary calcification, its relationship to local or systemic disease processes, and to renal stone formation, remain controversial.The object of this study is to investigate factors which might affect the size of the calcium concentration gradient in the human renal medulla. The distribution of calcium is measured in kidneys obtained from 152 subjects at necropsy or operation, and these findings are correlated with clinical and pathological data obtained from the clinical records, post-mortem findings and an histological examination of the kidneys.Materials and Methods.-Renal tissue was obtained from 13 patients at operation and 139 subjects at necropsy. Operative specimens were analysed as they became available during the period of the investigation, and the kidneys selected for study at necropsy were chosen randomly, specimens being obtained within 72 hours of death. In addition to this group of 152 kidneys, specimens from 2 selected cases of hyperca1ca:mia were analysed and the results presented separately. In each kidney, small samples of renal tissue (2Cr50 mg. wet weight) were dissected from the tip of the papilla (P), base of medulla (M) and mid-cortex (C); each was ashed, and then dissolved in N/50 nitric acid. The calcium concentration of these solutions was measured by atomic absorption spectrophotometry after the addition of lanthanum, and the calcium concentration of tissue samples calculated as mM Ca++/kg. wet tissue (Cooke, 1971). In view of sample variation, and in order to increase the accuracy of the method in comparing the distribution in different kidneys, 3 samples from each region were measured in the individual kidneys, and the means of the 3 results quoted. Within the group of 152 kidneys were 20 from s...

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