Neonatal porcine pancreas as a source of islet transplantation

Juang, Jyuhn‐Huarng; Hsu, Brend Ray‐Sea; Kuo, Chun‐Yan; Fu, Shin-Huei; Lee, W.-C; Yao, Nan-Kuang

doi:10.1016/s0041-1345(00)02239-9

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…The animal numbers, both for pigs and nude mice, used in each condition are described in each Figure accordingly. Donor pancreata were obtained from 1- to 3-day-old neonatal pigs of either gender following a previously described procedure 4 , 9 . Male athymic nude Balb/c mice aged 8–12 weeks, without contamination with relevant pathogens, were obtained from The National Laboratory Animal Center, Taiwan as recipients of the NPCCs.…”

Section: Methodsmentioning

confidence: 99%

“…At 6, 9, 16, 23, 30 and 60 days post Tx, mice were anesthetized with amobarbital and an abdominal incision was made to expose kidneys. Under a dissecting microscope, the graft was removed, fixed and prepared as paraffin-embedded tissue blocks for histological analysis 9 .…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies show that NPCCs were capable of restoring normoglycaemia in diabetic animals, which are mainly due to β cell expansion and differentiation of residing islet precursors into β cells 5 , 6 . Nevertheless, the fact that NPCCs could reverse hyperglycemia in diabetic mice only until 2 months post-Tx implies that NPCCs are rather immature and possess poor glucose-responsive insulin secretion even though NPCCs could secrete significant quantities of insulin in response to a steady-state glucose challenge in vitro 4 , 7 – 9 .…”

Section: Introductionmentioning

confidence: 99%

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Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Li¹,

Chen

Kao

et al. 2018

Sci Rep

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Ductal epithelium is primarily detected in porcine neonatal pancreatic cell clusters (NPCCs) bearing grafts, suggesting that transplants might exhibit progenitor-like phenotypes. Here we found that soon after NPCC isolation, PDX1+/insulin− and SOX9+ pancreatic progenitor-like cells dramatically increased while dual-hormonal progenitor-like cells were routinely observed in NPCC culture. After transplantation (Tx), insulin+ cells increased and PDX1+ and SOX9+ cells gradually decreased in both non-diabetic (NDM) and streptozotocin-induced diabetic (DM) grafts over 2 months. Strikingly, a significantly higher percentage of insulin+ cells were detected in 9-day and 16-day, but not in 23-day, 30-day and 60-day grafts implying that hyperglycemia could only facilitate NPCC-derived β cells early post-Tx. A higher percentage of NPCC-derived β cells in early DM grafts was determined via an enhanced neogenic differentiation based on the detection of insulin+ cells budding out from PDX1+/SOX9+ epithelium. Interestingly, a drop in SOX9+ progenitor-like cells was detected 16 days post-Tx in DM grafts whilst PDX1+ cells do not show a significant difference until 60 days post-Tx between DM and NDM grafts, demonstrating that distinct progenitor-like populations fuel new β cells post-Tx. In conclusion, PDX1+/SOX9+ cells could be quickly activated after NPCC isolation, maintain their multipotency in culture and differentiate into new β cell post-Tx.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Li¹,

Chen

Kao

et al. 2018

Sci Rep

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“…Therefore, a potential solution to these problems is the use of non-human donor islets. Pigs are considered to be the species of choice as a potential xenogeneic organ or tissue graft donor to humans, because pigs 1) are easy to breed, 2) grow and mature rapidly, 3) have a physiology remarkably like that human, 4) have established specific pathogen free (SPF) breeding, and 5) porcine insulin differs from human insulin only as regards one amino acid and 6) had routinely used to treat diabetic patients [9,12,21,23].…”

mentioning

confidence: 99%

Xenotransplantation of Porcine Pancreatic Endocrine Cells to Total Pancreatectomized Dogs

Edamura

Itakura

Nasu

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Xenotransplantation of porcine pancreatic endocrine (PE) cells in a diffusion chamber, a bioartificial endocrine pancreas (Bio-AEP), was conducted to total pancreatectomized dogs. Six pancreatectomized dogs were divided into two groups of 3 dogs each. In three dogs of the control group, exogenous insulin was administered twice a day for 30 weeks to maintain fasting blood glucose (FBG) levels within the normal range. The remaining three dogs were implanted with Bio-AEPs (implantation group), in addition to daily insulin administration. In the implantation group, Bio-AEPs containing 1.3 to 1.8 × 10 7 cells per kg of body weight of the recipient were implanted without fixation into the abdominal cavity. In the control group, exogenous insulin requirements did not decrease during the experimental period, whereas it significantly decreased for a certain period (3, 11, 17 weeks) after implantation in all implanted dogs. In the implantation group, laparotomy was performed after FBG and the exogenous insulin requirement increased again and Bio-AEPs were removed. Two Bio-AEPs were completely destroyed, and the remaining one was encapsulated by thin fibrous tissue. In this dog, effusion was present within the capsule, but the Bio-AEP was not destroyed. Histopathologically, the necrosis, presumably caused by hypoxia, of the PE-cells was observed on transmission electron microscopy. In conclusion, Bio-AEP could function for a certain period after implantation in this study. However, more preclinical researches should be needed to apply this technique for the treatme nt of diabetic dogs.

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Promotion of β-cell differentiation by the alkaloid conophylline in porcine pancreatic endocrine cells

Kawakami

Hirayama

Tsuchiya

et al. 2010

Biomedicine & Pharmacotherapy

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Neonatal porcine pancreas as a source of islet transplantation

Cited by 8 publications

References 14 publications

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Porcine Neonatal Pancreatic Cell Clusters Maintain Their Multipotency in Culture and After Transplantation

Xenotransplantation of Porcine Pancreatic Endocrine Cells to Total Pancreatectomized Dogs

Promotion of β-cell differentiation by the alkaloid conophylline in porcine pancreatic endocrine cells

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