Neonatal Hyperbilirubinemia. Evidence for a Role of the Erythrocyte Enzyme Activities Involved in the Detoxification of Oxygen Radicals

Bracci, Rodolfo; Buonocore, Giuseppe; Talluri, B.; Berni, Silvia

doi:10.1111/j.1651-2227.1988.tb10659.x

Cited by 35 publications

(25 citation statements)

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“…It has been reported in literature that jaundice produces an oxidative stress as demonstrated by decreased levels of cellular glutathione, gluthathione peroxidase and SOD in various studies (15,16). Antioxidant status in jaundiced neonates after phototherapy is not clear.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant status in neonatal jaundice before and after phototherapy

Dahiya

Tiwari

Shankar

et al. 2006

Indian J Clin Biochem

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Phototherapy has been related to increased oxidative stress and lipid peroxidation. In the present study, thirty full term jaundiced neonates with appropriate weight were analyzed before and after completion of phototherapy for malondialdehyde (MDA), reduced glutathione (GSH), total thiols, vitamin C and superoxide dismutase (SOD) levels in hemolysate and albumin levels in plasma. These parameters were analyzed in cord blood samples of 20 healthy neonates as control. It was observed that levels of MDA were elevated significantly (p<0.001) in patients as compared to controls and that the levels increased significantly after phototherapy (p<0.001). Levels of SOD were also found to be increased significantly as compared to controls and the levels rose after phototherapy (p<0.001). On the other hand, the levels of non-enzymatic antioxidants such as GSH, total thiols and vitamin C were significantly low (p<0.001) as compared to controls and the levels decreased significantly after phototherapy (p<0.001). The plasma albumin levels also were found to be decreased significantly after phototherapy (p<0.01). Therefore, phototherapy increases oxidative stress and should be used with care.

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Section: Resultsmentioning

confidence: 99%

Antioxidant status in neonatal jaundice before and after phototherapy

Dahiya

Tiwari

Shankar

et al. 2006

Indian J Clin Biochem

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“…intermediates ( 17), and changes of RBC population ( 18) have been reported to occur shortly after birth, other factors possibly involved in the protection of the cell from oxidative hemolysis, such as the characteristics of their membrane structure, have not been fully investigated.…”

Section: Ns Nsmentioning

confidence: 99%

Changes in Erythrocyte Properties during the First Hours of Life: Electron Spin Resonance of Reacting Sulfydryl Groups

et al. 1988

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ABSTRACT. In an attempt to clarify the mechanism by which the red cells (RBC) of newborn infants are protected against oxidative agents, electron spin resonance (ESR) assays were carried out using the nitroxide radical 4-maleimide-2,2,6,6-tetramethylpiperidinyl-1-01 (Mald), a sulfydryl-reacting agent. The ESR assays were performed in 24 samples of cord blood, 20 samples of blood from 4-day-old infants, and eight samples of &h-old infants. The analyses were carried out on whole blood and washed erythrocytes were resuspended in buffered saline. The same experiments were performed in 10 blood samples from healthy adults as controls. Whole blood, before and after removing the buffy coat, and cell-free plasma were also examined by ESR assay. Cell-free plasma and buffy coats proved not to be appreciably involved in the Ma14 behavior. The data of the ESR spectroscopy demonstrated a significantly slower reaction rate in the samples of cord blood and in blood of 8-h-old infants, compared to that of 4-day-old infants and adults. No significant differences in Mal-6 behavior could be detected between cord blood and 4-day-old infant blood in the results of ESR assays performed in washed red cells. Chemical determination of RBC-reacting sulfydryl groups and the assays of glutathione also demonstrated the absence of differences between cord blood and blood of 4-day-old infants. The results of our investigation suggest that the RBC-sulfydryl-reacting groups are less involved in the detoxification of oxidative agents during the first hours of life than in the following days. This peculiarity of RBC of younger infants appears to be due, to a considerable extent, to the modulation by plasma factors of the interactions between Mal-6 and RBCreacting sulfydryl groups. Therefore, the changes in plasma components occurring during the first hours of life appear to modify the interactions between the RBC and the oxidative agents. (Pediatr Res 24: 391-395, 1988) Abbreviations RBC, red blood cells ESR, electron spin resonance -SH, sulfydryl-reacting groups Mal-6,4-maleimide-2,2,6,6-tetramethylpiperidiny1-1-oxy1 Htc, hematocrit GSH, glutathione

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“…Also, in another study, authors have shown that UCB induces a dosedependent increase in neuronal death in parallel with the oxidation of cell components and a decrease in the intracellular glutathione content 4 . Bracci et al (1988) showed that antioxidant enzymes of erythrocyte were significantly lower in cord blood and on the 4th day of life in babies with high bilirubinemia compared to less jaundiced babies 48 . Recently, Dani et al (2003) have shown a decrease plasma bilirubin level and oxidative stress concominant with an increase in plasma antioxidant capacity in the preterm infants 49 .…”

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confidence: 99%