Neomycin ototoxicity

Thomas, Jon; Marion, Mitchell S.; Hinojosa, Raúl

doi:10.1016/0196-0709(92)90099-f

Cited by 6 publications

(1 citation statement)

References 3 publications

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“…A1-though relatively toxic compared with other classes of antibiotics, they remain useful primary in the treatment of infections caused by aerobic gram-negative bacteria [15,16]. Al1 aminoglycosides have the potential to produce reversible vestibular, cochlear, and renal toxicity [17][18][19][20]. Their ototoxic effects may occur after parenteral administration, aerosolisation, topical use, wound or cavity irrigation and topical otic administration [21].…”

Section: Introductionmentioning

confidence: 99%

Application of EPR spectroscopy to examination of gentamicin and kanamycin binding to DOPA-melanin

et al. 2002

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Electron paramagnetic resonance (EPR) spectroscopy was applied to measure the influence of two aminoglycoside antibiotics: gentamicin and kanamycin on free radical properties of DOPAmelanin. DOPA-melanin was formed by oxidative polymerization of 3,4-dihydroxyphenylalanine. Different concentrations of gentamicin and kanamycin (from 1 9 10 -4 to 1 9 10 -2 M) were used. o-Semiquinone free radicals with a g factor of 2.0043 were found in all studied melanin samples. Their concentrations in the DOPA-melanin-drug complexes were higher than in DOPA-melanin, and increased with the increase of gentamicin and kanamycin concentration. A single EPR line of the analyzed samples (ABp~, 0.48-0.52 mT) indicates that aminoglycoside antibiotics do not create a new type of free radicals in DOPA-melanin. Microwave saturation behavior of the experimental lines indicates the homogeneous broadening of resonance absorption curves for DOPA-melanin and its complexes with aminoglycosides. The EPR lines saturate at low microwave powers. Slow spin-lattice relaxation processes were characteristic for all studied melanin samples.

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Section: Introductionmentioning

confidence: 99%

Application of EPR spectroscopy to examination of gentamicin and kanamycin binding to DOPA-melanin

et al. 2002

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Basic fibroblast growth factor (FGF-2) protects rat cochlear hair cells in organotypical culture from aminoglycoside injury

et al. 1996

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Given the evidence that basic fibroblast growth factor (FGF-2) can protect neural and retinal cells from degeneration, we evaluated the potential of this growth factor to protect sensory cells in the inner ear. When sensory cells of the organ of Corti are exposed to aminoglycoside antibiotics such as neomycin either in vivo or in vitro, significant ototoxicity is observed. The in vitro cytotoxic effects of neomycin are dose and time dependent. In neonatal rat organ of Corti cultures, complete inner and outer hair cell destruction is observed at high (mM) concentrations of neomycin while inner hair cell survival and severely damaged outer hair cells are noted at moderate (microM) concentrations, with a maximal effect observed after 2 days of culture. Approximately 50% of cochlear outer hair cells are lost at a dose of 35 microM neomycin, and most surviving cells show disorganized stereocilia. Inner hair cells show primarily disorganization of their stereocilia. A significant protective effect is observed when the organ of Corti is pre-treated with FGF-2 (500 ng/ml) for 48 hours, and then FGF-2 is included with neomycin in the culture medium. A greater extent of outer hair cell survival and a significant decrease in stereociliary damage are noted with FGF-2. However, disorganization of inner hair cell stereocilia is unaffected by FGF-2. The protective effect of FGF-2 is specific, since interleukin-1B, nerve growth factor, tumor necrosis factor, and epidermal growth factor are ineffective, while retinoic acid and transforming growth factor alpha show only a moderate protective effect. These results confirm the potential of molecules like FGF-2 for preventing cell death due to a variety of causes.

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Ototoxicity

Gotthelf¹

2005

Small Animal Ear Diseases

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Neomycin ototoxicity

Cited by 6 publications

References 3 publications

Application of EPR spectroscopy to examination of gentamicin and kanamycin binding to DOPA-melanin

Application of EPR spectroscopy to examination of gentamicin and kanamycin binding to DOPA-melanin

Basic fibroblast growth factor (FGF-2) protects rat cochlear hair cells in organotypical culture from aminoglycoside injury

Ototoxicity

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