Nematicidal effect of chitosan on Meloidogyne incognita in vitro and on tomato in a pot experiment

Abstract

The present study investigated to evaluate the potential of liquid chitosan of three concentrations (0.5, 1 and 2%) on Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 in vitro and on tomato under controlled conditions ((24 ± 1 °C, 60 ± 5% humidity). In vitro assay, the effect of the liquid chitosan concentrations on suppressing of hatching from eggs and second juvenile larvae (J2) mortality was determined. The reducing effect of the concentrations on the number of gall and egg mass on tomato roots and the J2 density in the soil was evaluated under controlled conditions. All concentrations suppressed hatch and increased J2 mortality more than control in vitro. The most effective concentration was found at 2% in vitro and its nematicidal effect on egg and J2 was over 70%. The results demonstrated that 0.5, 1 and 2% concentrations were significantly decreased gall/root, egg mass/root and J2 in soil compared to negative control under controlled conditions. No statistically significant difference was found between the nematicidal effects of the concentrations on the gall and egg mass (P≤0.05). It has been determined that 1 and 2% concentrations better suppress the J2 in soil than 0.5%. Although the nematicidal effect of 2% concentration was high in vitro and under controlled conditions, it was determined that it negatively affected plant biomass. Also, only 1% concentration of chitosan application controlled M. incognita on tomato by 58%. The present results show that the use of 1% liquid chitosan concentration against M. incognita will be more effective.

Chitin-enriched insect frass fertilizer as a biorational alternative for root-knot nematode (Meloidogyne incognita) management

“…The mechanism of egg hatch inhibition by chitin may be related to the influx of toxic compounds through the nematode eggshell upon contact with chitin contained in the chFE or the deterioration of the nematode eggshell leading to in situ mortality of the unhatched juveniles ( Mota and dos Santos, 2016 ; Göze Özdemir et al., 2022 ). In our study, we observed no difference in the inhibition of egg hatchability between the commercial nematicide and the chFE E30, E40, E50 and E100 at 15 days post exposure, an indication that chFE with ≥3% chitin composition is optimal for suppressing M. incognita egg hatchability, and thus, chFE could be a potential and environmentally friendly alternative to synthetic nematicides.…”

Synthesis of Nano Sulfur/Chitosan-Copper Complex and Its Nematicidal Effect against Meloidogyne incognita In Vitro and on Coffee Pots

“…The experiment was carried out following the method of El-Ashry et al (2022) and Özdemir et al (2022) with several modifications. Robusta coffee seeds ( Coffea canephora ) were sterilized by soaking it in NaClO solution for 20 min and washed with running tap water 3 times.…”

Fabrication and characterization of inorganic–organic hybrid copper ferrite anchored on chitosan Schiff base as a reusable green catalyst for the synthesis of indeno[1,2-b]indolone derivatives

“… 18 The existence of interactions between metal and OH groups on the surface of chitosan has recently attracted increasing attention for the fabrication of complex materials. 19 It is due to the presence of NH 2 on the surface of chitosan that it allows copolymerization using monomers to achieve unique properties. 20 Also, the reason for using magnetic nanoparticles in the synthesis of the catalyst can be attributed to their two general characteristics: effects that are limited to the size of their particles and effects that are superficial and cause special characteristics in magnetic nanoparticles.…”