Nematicidal activity of grammicin produced by <i>Xylaria grammica</i> KCTC 13121BP against <i>Meloidogyne incognita</i>

Kim, Tae Yoon; Jang, Ja Yeong; Yu, Nan Hee; Jae, Won Yong; Bae, Chang−Hwan; Yeo, Joo Hong; Park, Ae Ran; Hur, J.S.; Park, Hae Woong; Park, Jiyeon; Park, Jong‐Hwan; Lee, Sung Ki; Kim, Jin-Cheol

doi:10.1002/ps.4717

Cited by 46 publications

(44 citation statements)

References 27 publications

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“…The experiment was conducted in a melon field naturally infected with RKNs ( Meloidogyne spp.) located in Gyuam‐myeon, Buyeo, Chungnam, Korea from July to September 2016 . Melon seedlings (cv.…”

Section: Methodssupporting

confidence: 87%

“…Up to 50 J2 individuals of Meloidogyne spp. or mixed stage B. xylophilus were placed in the wells of 96‐well tissue culture plates (Becton Dickinson, Franklin Lakes, NJ, USA) . Distilled water containing 1% organic solvent served as a negative control, and abamectin (Supelco, Bellefonate, PA, USA), a natural nematicide, was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate the potential of using EtOAc extracts from W. indica as bio‐control agents, a wettable powder‐type formulation was prepared as previously described by Kim et al The EtOAc layer (2 g) of W. indica was dissolved in a minimal volume of acetone and then mixed with 1.5 g of synthetic hydrated silicon dioxide (white carbon; Rhodia Asia Pacific Pte Ltd, The Concourse, Singapore). Acetone was removed in a fume hood, and then white carbon was mixed with 0.5 g of sodium dodecyl sulfate (CR‐SDS; Yoosung Chemical R&T Co. Ltd, Daejeon, Korea) as a wetting agent, 0.4 g of sodium polysulfonate (CR‐WP100; Yoosung Chemical R&T) as a dispersal agent, and 5.5 g of kaolin.…”

Section: Methodsmentioning

confidence: 99%

“…The severity of root gall was assessed using a 0–5 scale, as described above, according to the percentage of galled tissue. The experiment was conducted once with three replicates …”

Section: Methodsmentioning

confidence: 99%

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Control of root‐knot nematodes using Waltheria indica producing 4‐quinolone alkaloids

Jang

Dang²,

Choi

et al. 2019

Pest Management Science

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BACKGROUND: Waltheria indica exhibited strong nematocidal activity against Meloidogyne incognita, a causal agent of root-knot nematode disease. This study aimed to characterize the nematocidal metabolites and to evaluate the efficacy of the formulated extract of W. indica in the biological control of M. incognita under both pot and field conditions. W. indica (W. indica WP20) at 26.7, 53.4 and 106.8 mg a.i. kg −1 soil significantly reduced the formation of galls and egg masses on the roots of tomato plants in a dose-dependent manner. In addition, application of 20 mg a.i. per plant W. indica WP20 effectively reduced gall formation on the roots of melon plants and population density of nematode in soil compared with untreated control under field conditions. CONCLUSION: W. indica can be used as an effective botanical nematicide in the eco-friendly control of root-knot nematode disease. RESULTS: Three 4-quinolone alkaloids, 5 ′ -methoxywaltherione A, waltherione A and waltherione C, were isolated and characterized as nematocidal metabolites. 5 ′ -Methoxywaltherione A and waltherione A caused high mortality in juveniles of Meloidogyne arenaria, Meloidogyne hapla, M. incognita and Bursaphelenchus xylophilus, whereas waltherione C exhibited significant nematocidal activity against only root-knot nematodes. In pot experiments, application of a wettable powder-type formulation of the ethyl acetate extract of

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Section: Methodssupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The severity of root gall was assessed using a 0–5 scale, as described above, according to the percentage of galled tissue. The experiment was conducted once with three replicates …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Control of root‐knot nematodes using Waltheria indica producing 4‐quinolone alkaloids

Jang

Dang²,

Choi

et al. 2019

Pest Management Science

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“…Even though the in vitro antibacterial activity of compound 1 was not as strong as that of other fungi‐derived metabolites such as patulin (Kim et al . ), the crude extract EtOAc layer showed relatively high in vitro antibacterial activity with MIC values of 31·2–500 μ g ml −1 against 13 phytopathogenic bacteria among the 14 tested. In addition, both the EtOAc layer and compound 1 effectively controlled the development of tomato bacterial wilt in a pot experiment.…”

Section: Discussionmentioning

confidence: 93%

Antimicrobial activities of an oxygenated cyclohexanone derivative isolated from Amphirosellinia nigrospora JS-1675 against various plant pathogenic bacteria and fungi

Nguyen

Kim

et al. 2019

J Appl Microbiol

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Aims To evaluate the antimicrobial activities of an active compound isolated from the culture broth of Amphirosellinia nigrospora JS‐1675 against various plant pathogenic bacteria and fungi. Methods and Results While screening for bioactive secondary metabolites from endophytic fungi, we found that A. nigrospora JS‐1675 showed strong in vitro antibacterial activity against Ralstonia solanacearum. One compound (1) was isolated and identified as (4S, 5S, 6S)‐5,6‐epoxy‐4‐hydroxy‐3‐methoxy‐5‐methyl‐cyclohex‐2‐en‐1‐one. Growth of most of the tested phytopathogenic bacteria was inhibited by compound 1 and the ethyl acetate (EtOAc) layer except Pseudomonas syringae pv. lachrymans. Compound 1 also inhibited the mycelial growth of several plant pathogenic fungi. Both compound 1 and the EtOAc layer reduced bacterial leaf spot disease in detached peach leaves. They also suppressed the development of bacterial wilt on tomato seedlings quite effectively. Conclusions Amphirosellinia nigrospora JS‐1675 showed antimicrobial activity against plant pathogenic bacteria and fungi by producing compound 1. Significance and Impact of the Study This is the first report on the occurrence of compound 1 in A. nigrospora JS‐1675 and its efficacy against plant pathogenic bacteria and fungi. Their strong disease control efficacy against tomato bacterial wilt suggests that this fungus can be used as a microbial bactericide.

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Biocontrol of the root‐knot nematodeMeloidogyne incognitaby a nematicidal bacteriumPseudomonas simiaeMB751with cyclic dipeptide

Sun

Zhang

Ding

et al. 2021

Pest Management Science

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BACKGROUND Root‐knot nematodes (RKNs) are harmful plant‐parasitic nematodes that cause serious damage to plant hosts. In the long‐term practice of RKN management, bacterial nematicides have attracted increasing attention as an effective biocontrol means. Here we determined the active substances against Meloidogyne incognita from a nematicidal bacterium, developed a biocontrol agent (BCA) based on optimized culture processes. The effects of the BCA on RKN control and plant growth‐promotion were evaluated in tomato pot trials. RESULTS Pseudomonas simiae strain MB751 exhibiting significant nematicidal activity against M. incognita second‐stage juveniles (J2) with approximately 80% mortality (with culture supernatant, 96% volume percentage) was isolated from a vineyard. A set of purification and identification experiments was performed to determine the main nematicidal component in MB751. A cyclic dipeptide Cyclo(L‐Pro‐L‐Leu) was identified with a lethal concentration necessary to kill 50% of the population (LC50) of 65.3 μg mL−1 against M. incognita J2. Following optimization trials on culture medium/fermentation conditions, such as the single factor test, Plackett–Burman test, steepest ascent, and response surface methodology experiments, the MB751 fermentation broth was then prepared as a BCA via a cold‐air drying process. The BCA and was evaluated in tomato pot experiments for effectiveness in suppressing M. incognita. Significant effects on M. incognita suppression and plant‐growth promotion as well as induced systemic resistance to M. incognita of tomato, were observed. CONCLUSION The cyclic dipeptide‐producing bacterium P. simiae MB751 exhibited high nematicidal activity and performance. Further development of this BCA should be pursued for the management of M. incognita in agriculture. © 2021 Society of Chemical Industry.

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Nematicidal activity of grammicin produced by Xylaria grammica KCTC 13121BP against Meloidogyne incognita

Abstract: The results suggest that X. grammica and grammicin may have potential applications for control of root-knot nematode disease of various crops. © 2017 Society of Chemical Industry.

Cited by 46 publications

References 27 publications

Control of root‐knot nematodes using Waltheria indica producing 4‐quinolone alkaloids

Control of root‐knot nematodes using Waltheria indica producing 4‐quinolone alkaloids

Antimicrobial activities of an oxygenated cyclohexanone derivative isolated from Amphirosellinia nigrospora JS-1675 against various plant pathogenic bacteria and fungi

Biocontrol of the root‐knot nematodeMeloidogyne incognitaby a nematicidal bacteriumPseudomonas simiaeMB751with cyclic dipeptide

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