Nematicidal Activity of Bikaverin and Fusaric Acid Isolated from Fusarium oxysporum against Pine Wood Nematode, Bursaphelenchus xylophilus

Kwon, Hyeok Ran; Son, Seung‐Woo; Han, Hee-Jeong; Choi, Gyung Ja; Jang, Kyoung Soo; Choi, Yong Ho; Lee, Sun Og; Sung, Nack Do; Kim, Jin Cheol

doi:10.5423/ppj.2007.23.4.318

Cited by 46 publications

(32 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, Lewis and Paul (1981) and Kato et al (1977) demonstrated other synthetic routes for formation of bikaverin. Bikaverin exhibits a variety of biological activities: it had antibiotic activity against Leishmania brasiliensis (Balan et al 1970), antifungal activity against filamentous fungi (Cornforth et al 1971), anti-oomycete against Phytophthora infestans (Son et al 2008), and nematicidal activity against Bursaphelenchus xylophilus (Kwon et al 2007). Bikaverin also exhibits the cytotoxic activity against rat primary hepatocytes (Norred et al 1992;Vesonder et al 1989).…”

Section: Introductionmentioning

confidence: 97%

LC-MS/MS method for the determination of the fungal pigment bikaverin in maize kernels as an indicator of ear rot

Busman

Butchko

Proctor

2012

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

Bikaverin is a polyketide-derived pigment produced by multiple species of the fungus Fusarium, some of which can cause ear and kernel rot of maize. A method was developed for the analysis of bikaverin by high-performance liquid chromatography (LC) coupled to electrospray ionisation tandem mass spectrometry (MS/MS). The quantitative nature of the LC-MS/MS method was demonstrated over a range of concentrations of bikaverin in maize. For spike-recovery experiments utilising maize spiked with bikaverin to a level 5 µg g⁻¹ of maize, the measured recovery (%) was 70.6 ± 10.4. Based on the utilised method, the limit of detection (based on a signal-to-noise ratio (S/N) > 3) was better than 0.5 µg g⁻¹ from bikaverin spiked into uncontaminated ground maize. Further, the limit of quantitation (LOQ) was 3 µg g⁻¹ (based on S/N > 10) from bikaverin spiked into ground maize. The method was applied to assess contamination of maize with bikaverin following inoculation of developing maize ears with Fusarium verticillioides under agricultural field conditions.

show abstract

Section: Introductionmentioning

confidence: 97%

LC-MS/MS method for the determination of the fungal pigment bikaverin in maize kernels as an indicator of ear rot

Busman

Butchko

Proctor

2012

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

show abstract

“…Bikaverin is a polyketide compound, frequently biosynthesized by various Fusarium species (Bell et al 2003). It exhibited a broad spectrum of bioactivity such as an antibiotic effective against Leishmania braziliensis (Balan et al 1970), nematicidal activity against the pine wood nematode Bursaphelenchus xylophilus (Kwon et al 2007), and antioomycete activity in the control of tomato late blight disease (Son et al 2008). Bikaverin actively uncoupled oxidative phosphorylation of tumor cells and of rat liver mitochondria (Kovac et al 1978).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

et al. 2014

View full text Add to dashboard Cite

The generation of free radicals and oxidative stress has been linked to several neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease, and Amyotrophic lateral sclerosis. The use of free radical scavenging molecules for the reduction of intracellular reactive oxygen species is one of the strategies used in the clinical management of neurodegeneration. Fungal secondary metabolism is a rich source of novel molecules with potential bioactivity. In the current study, bikaverin was extracted from Fusarium oxysporum f. sp. lycopersici and its structural characterization was carried out. Further, we explored the protective effects of bikaverin on oxidative stress and its anti-apoptotic mechanism to attenuate H 2 O 2 -induced neurotoxicity using human neuroblastoma SH-SY5Y cells. Our results elucidate that pretreatment of neurons with bikaverin attenuates the mitochondrial and plasma membrane damage induced by 100 lM H 2 O 2 to 82 and 26 % as evidenced by MTT and LDH assays. H 2 O 2 induced depletion of antioxidant enzyme status was also replenished by bikaverin which was confirmed by Realtime Quantitative PCR analysis of SOD and CAT genes. Bikaverin pretreatment efficiently potentiated the H 2 O 2 -induced neuronal markers, such as BDNF, TH, and AADC expression, which orchestrate the neuronal damage of the cell. The H 2 O 2 -induced damage to cells, nuclear, and mitochondrial integrity was also restored by bikaverin. Bikaverin could be developed as a preventive agent against neurodegeneration and as an alternative to some of the toxic synthetic antioxidants.

show abstract

“…Indeed, FA was found to be able to chelate divalent cations, especially Zn and inactivate Zn finger proteins involved in DNA repair and protein synthesis [19,23]. In contrary, other studies mentioned that the relative low concentrations of FA could induce typical early defense response such as reactive O species 2 (ROS production) which involved in achieving biocontrol and nematicidal potential against some hazardous parasites as pine wood nematode Bursaphelenchus xylophilus [20].…”

Section: Discussionmentioning

confidence: 97%

“…FA is a natural mycotoxin produced by numerous Fusarium species under both in vitro and in vivo conditions [3,16,18]. Large concentrations of FA (greater than 10 M) were found to be extremely toxic to all living organisms including host plants, animals and human beings [4] while the least concentrations show antibiotic, insecticidal and pharmacological potentials [19,20].…”

Section: Discussionmentioning

confidence: 99%

Role of Fusaric Acid Mycotoxin in Pathogensis Process of Tomato Wilt Disease Caused by Fusarium oxysporum

Selim¹,

El-Gammal²

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

Nematicidal Activity of Bikaverin and Fusaric Acid Isolated from Fusarium oxysporum against Pine Wood Nematode, Bursaphelenchus xylophilus

Cited by 46 publications

References 12 publications

LC-MS/MS method for the determination of the fungal pigment bikaverin in maize kernels as an indicator of ear rot

LC-MS/MS method for the determination of the fungal pigment bikaverin in maize kernels as an indicator of ear rot

Neuroprotective Effects of Bikaverin on H2O2-Induced Oxidative Stress Mediated Neuronal Damage in SH-SY5Y Cell Line

Role of Fusaric Acid Mycotoxin in Pathogensis Process of Tomato Wilt Disease Caused by Fusarium oxysporum

Contact Info

Product

Resources

About