Neisserial pilin genes display extensive interspecies diversity

Aho, Ellen L.; Urwin, Rachel; Batcheller, A.E.; Holmgren, Alicia M.; Havig, Kelly; Kulakoski, Amy M.; Vomhof, Emilie E.; Longfors, Nancy S.; Erickson, Christopher B.; Anderson, Zacchary K.; Dawlaty, Jahan M.; Mueller, Joshua J

doi:10.1016/j.femsle.2005.06.035

Cited by 14 publications

(20 citation statements)

References 47 publications

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“…The G +C content of pilin alleles has been previously reported as a means to provide information concerning the evolution of pilA loci by gene transfer/ recombination events in other bacterial including P. aeruginosa and Neisseria spps. (Castric and Deal, 1994;Spangenberg et al, 1995;Kus et al, 2004;Aho et al, 2005). In accordance with these reports, the G+C content of the M. catarrhalis pilA further distinguished the two clades.…”

Section: Sequence Comparisons Of Representative Pila From Several M supporting

confidence: 74%

Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Luke-Marshall

Sauberan

Campagnari

2011

Gene

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Section: Sequence Comparisons Of Representative Pila From Several M supporting

confidence: 74%

Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Luke-Marshall

Sauberan

Campagnari

2011

Gene

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“…PilE is the major constituent of Tfp and some commensal Neisseria have been reported to contain two putative pilE genes (Aho et al, 2005;Marri et al, 2010). Here, we present evidence that, of the two putative pilE genes in N. cinerea 346T, pilE1 is transcribed and does not appear to form a co-transcriptional unit with pilE2.…”

Section: Discussionmentioning

confidence: 60%

Neisseria cinerea isolates can adhere to human epithelial cells by type IV pilus-independent mechanisms

et al. 2016

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In pathogenic Neisseria species the type IV pili (Tfp) are of primary importance in hostpathogen interactions. Tfp mediate initial bacterial attachment to cell surfaces and formation of microcolonies via pilus-pilus interactions. Based on genome analysis, many non-pathogenic Neisseria species are predicted to express Tfp, but aside from studies on Neisseria elongata, relatively little is known about the formation and function of pili in these organisms. Here, we have analysed pilin expression and the role of Tfp in Neisseria cinerea. This non-pathogenic species shares a close taxonomic relationship to the pathogen Neisseria meningitidis and also colonizes the human oropharyngeal cavity. Through analysis of non-pathogenic Neisseria genomes we identified two genes with homology to pilE, which encodes the major pilin of N. meningitidis. We show which of the two genes is required for Tfp expression in N. cinerea and that Tfp in this species are required for DNA competence, similar to other Neisseria. However, in contrast to the meningococcus, deletion of the pilin gene did not impact the association of N. cinerea to human epithelial cells, demonstrating that N. cinerea isolates can adhere to human epithelial cells by Tfp-independent mechanisms.

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“…Most Neisseria have a single pilE locus, but in some cases, tandem alleles (pilE1 and pilE2) have been reported (6). The number of pilS alleles varies between strains, as 17 silent copies were identified in N. gonorrhoeae strain MS11, while 19 different pilS loci at 6 distinct chromosomal locations were identified in N. gonorrhoeae strain FA1090 (161,165).…”

Section: Diversity In Neisseria Pilinsmentioning

confidence: 99%

“…In addition to the pilins described above, which are referred to as class I pilins, specific clonal complexes of N. meningitidis express a second T4aP pilin type, designated class II (6,88). Class II pilins are smaller (15 to 17 kDa) and do not bind the monoclonal antibody SM1, which recognizes both N. gonorrhoeae and N. meningitis class I pilins.…”

Section: Diversity In Neisseria Pilinsmentioning

confidence: 99%

Type IV Pilin Proteins: Versatile Molecular Modules

Giltner

Nguyen

Burrows

2012

Microbiol Mol Biol Rev

401

519

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SUMMARYType IV pili (T4P) are multifunctional protein fibers produced on the surfaces of a wide variety of bacteria and archaea. The major subunit of T4P is the type IV pilin, and structurally related proteins are found as components of the type II secretion (T2S) system, where they are called pseudopilins; of DNA uptake/competence systems in both Gram-negative and Gram-positive species; and of flagella, pili, and sugar-binding systems in the archaea. This broad distribution of a single protein family implies both a common evolutionary origin and a highly adaptable functional plan. The type IV pilin is a remarkably versatile architectural module that has been adopted widely for a variety of functions, including motility, attachment to chemically diverse surfaces, electrical conductance, acquisition of DNA, and secretion of a broad range of structurally distinct protein substrates. In this review, we consider recent advances in this research area, from structural revelations to insights into diversity, posttranslational modifications, regulation, and function.

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Neisserial pilin genes display extensive interspecies diversity

Cited by 14 publications

References 47 publications

Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Neisseria cinerea isolates can adhere to human epithelial cells by type IV pilus-independent mechanisms

Type IV Pilin Proteins: Versatile Molecular Modules

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