Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Luke-Marshall, Nicole R.; Sauberan, Shauna L.; Campagnari, Anthony A.

doi:10.1016/j.gene.2011.01.010

Cited by 20 publications

(14 citation statements)

References 31 publications

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“…Recombinant Mcat proteins OMP CD [12], OppA [14], Hag5–9 (truncated Hag protein) [16], and PilA2 [17] were expressed and purified as previously described. We designed and constructed an expression plasmid for the gene of non-lipidated Msp22 which we named Msp22NL in a vector, pET303 with His-tag at its C-terminal.…”

Section: Methodsmentioning

confidence: 99%

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Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Ren

Almudevar

Murphy

et al. 2015

Vaccine

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Background There is no licensed vaccine for Moraxella catarrhalis (Mcat), which is a prominent bacterium causing acute otitis media (AOM) in children and lower respiratory tract infections in adults. Nasopharyngeal (NP) colonization caused by respiratory bacteria results in natural immunization of the host. To identify Mcat antigens as vaccine candidates, we evaluated the development of naturally induced antibodies to 5 Mcat surface proteins in children 6–30 months of age during Mcat NP colonization and AOM. Methods Human serum IgG against the recombinant Mcat proteins, outer membrane protein (OMP) CD, oligopeptide permease (Opp)A, hemagglutinin (Hag), Moraxella surface protein (Msp)22, and PilA clade 2 (PilA2) was quantitated by using an ELISA assay. Results There were 223 Mcat NP colonization episodes documented in 111 (60%) of 184 children in the study. Thirty five Mcat AOM episodes occurred in 30 (16%) of 184 children. All 5 Mcat candidate vaccine antigens evaluated stimulated a significant rise in serum IgG levles over time from 6 to 36 months of age (P < 0.001), with a rank order as follows: Msp22 = OppA > OMP CD = Hag = PilA2. Children with no detectable Mcat NP colonization showed a higher serum IgG level against OppA, Hag, and Msp22 compared to those with Mcat NP colonization (P < 0.05). Individual data showed that some children responded to AOM with an antibody increase to one or more of the studied Mcat proteins but some children failed to respond. Conclusions Serum antibody to Mcat candidate vaccine proteins OMP CD, OppA, Msp22, Hag, and PilA2 increased with age in naturally immunized children age 6–30 months following Mcat NP colonization and AOM. High antibody levels against OppA, Msp22, and Hag correlated with reduced carriage. The results support further investigation of these vaccine candidates in protecting against Mcat colonization and infection.

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Section: Methodsmentioning

confidence: 99%

“…It contains regions of highly conserved and moderately conserved domains [16]. PilA2 is the major pilin subunit that is conserved and essential for genetic transformation, adherence to eukaryotic cells and biofilm formation [17]. …”

Section: Introductionmentioning

confidence: 99%

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Ren

Almudevar

Murphy

et al. 2015

Vaccine

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“…The Q pilin is associated with increased binding to corneal tissue compared with the I pilin (337). Recent studies of Moraxella catarrhalis, an emerging human pathogen responsible for a large proportion of otitis media and other upper respiratory tract infections, showed a low level of pilin diversity (262). All strains tested expressed either clade 1 (PilA1; 42%) or clade 2 (PilA2; 58%) pilins, with PilA1 having an 11-residue deletion at the C terminus compared with PilA2.…”

Section: Moraxella and Dichelobacter Pilin Diversitymentioning

confidence: 99%

Type IV Pilin Proteins: Versatile Molecular Modules

Giltner

Nguyen

Burrows

2012

Microbiol Mol Biol Rev

401

519

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SUMMARYType IV pili (T4P) are multifunctional protein fibers produced on the surfaces of a wide variety of bacteria and archaea. The major subunit of T4P is the type IV pilin, and structurally related proteins are found as components of the type II secretion (T2S) system, where they are called pseudopilins; of DNA uptake/competence systems in both Gram-negative and Gram-positive species; and of flagella, pili, and sugar-binding systems in the archaea. This broad distribution of a single protein family implies both a common evolutionary origin and a highly adaptable functional plan. The type IV pilin is a remarkably versatile architectural module that has been adopted widely for a variety of functions, including motility, attachment to chemically diverse surfaces, electrical conductance, acquisition of DNA, and secretion of a broad range of structurally distinct protein substrates. In this review, we consider recent advances in this research area, from structural revelations to insights into diversity, posttranslational modifications, regulation, and function.

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“…Moreover, a specific pilA variant was associated with isolates causing keratitis [83], further supporting the role of this gene in disease development. In another study, pilA was used to divide the human pathogen Moraxella catharralis into two clades based mainly on DNA sequence divergence at the C -terminus [84]. Isolates from both clades did not differ in pathogenicity despite the diverse origins of isolation from different patients and geographic locations [84].…”

Section: Genes Involved In T4p Synthesis and Variability Of Pilinmentioning

confidence: 99%

“…In another study, pilA was used to divide the human pathogen Moraxella catharralis into two clades based mainly on DNA sequence divergence at the C -terminus [84]. Isolates from both clades did not differ in pathogenicity despite the diverse origins of isolation from different patients and geographic locations [84]. The authors concluded that, for this bacterium, this highly conserved gene is a good candidate as an antigen for vaccine development.…”

Section: Genes Involved In T4p Synthesis and Variability Of Pilinmentioning

confidence: 99%

Involvement of Type IV Pili in Pathogenicity of Plant Pathogenic Bacteria

Burdman

Bahar

Parker

et al. 2011

Genes

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Type IV pili (T4P) are hair-like appendages found on the surface of a wide range of bacteria belonging to the β-, γ-, and δ-Proteobacteria, Cyanobacteria and Firmicutes. They constitute an efficient device for a particular type of bacterial surface motility, named twitching, and are involved in several other bacterial activities and functions, including surface adherence, colonization, biofilm formation, genetic material uptake and virulence. Tens of genes are involved in T4P synthesis and regulation, with the majority of them being generally named pil/fim genes. Despite the multiple functionality of T4P and their well-established role in pathogenicity of animal pathogenic bacteria, relatively little attention has been given to the role of T4P in plant pathogenic bacteria. Only in recent years studies have begun to examine with more attention the relevance of these surface appendages for virulence of plant bacterial pathogens. The aim of this review is to summarize the current knowledge about T4P genetic machinery and its role in the interactions between phytopathogenic bacteria and their plant hosts.

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Comparative analyses of the Moraxella catarrhalis type-IV pilus structural subunit PilA

Cited by 20 publications

References 31 publications

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Type IV Pilin Proteins: Versatile Molecular Modules

Involvement of Type IV Pili in Pathogenicity of Plant Pathogenic Bacteria

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