Negative Pion Irradiation of Mammalian Cells

Weibezahn, Karl-Friedrich; Sexauer, C.; Coquerelle, Thérèse

doi:10.1080/09553008014551741

Cited by 17 publications

(2 citation statements)

References 9 publications

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“…DNA DSB are the result of irradiation. With increasing linear energy transfer of the radiation, cell killing and number of unrepairable DSB also increase (1,2). DSB thus seem to be life threatening damages.…”

Section: Introductionmentioning

confidence: 99%

Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes

Weibezahn¹,

Coquerelle²

1981

Nucl Acids Res

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Using the method of filter elution of double stranded DNA under neutral conditions we have shown that most of gamma-ray induced double strand breaks (DSB) are rejoined in both mammalian and bacterial cells. Rejoining also occurs in the G1 phase in V79 Chinese hamster cells and under different growth conditions. Within 8 minutes at 37 C, half the breaks are rejoined. The rejoining in E. coli is equally fast and depends on the presence of DNA ligase. Some of the breaks in E. coli rejoin slowly, and these require rec+. The non-rejoined DSB are distributed over the DNA without any preference for the nucleosomal or the linker structure in the chromosome. Two kinds of DSB rejoining are discriminated, a fast process of DNA ligation and a slower process involving rec functions.

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Section: Introductionmentioning

confidence: 99%

Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes

Weibezahn¹,

Coquerelle²

1981

Nucl Acids Res

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“…DNA dsb is a critical damage induced by ionizing radiation. Both cell killing and the number of unrepaired dsb are increased as a function of the dose delivered (10,11). Different rejoining processes can operate for the repair of dsb in irradiated cells (12,13).…”

Section: Introductionmentioning

confidence: 99%

Promotion of double-strand break repair by human nuclear extracts preferentially involves recombination with intact homologous DNA

Coppey

1987

Nucl Acids Res

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Parameters of DNA double strand break (dsb) repair catalysed by human nuclear extract were analysed using, as substrate, the replicative form (RF) of M13 mp8 in which a single double strand break (dsb) was introduced by restriction. After incubation with the extract, the dsb repair was estimated by the ability of the incubated RF to produce plaques following transfection into JM 109 (Rec A-) bacteria. The possibility of recombination with a purified fragment from M13 mp8 RF enhances up to 20 times the plaquing ability of the RF. The repair by recombination occurs under several conditions: i) the break in the RF must be located in the region of homology with the fragment. ii) the fragment has to be intact in the region corresponding to the break in the RF. iii) a minimal length of homology between the region surrounding the dsb in the RF, and the fragment is required. The in vitro reaction is ATP dependent and dNTP's partially dependent. Dephosphorylation of the free ends in the RF decreases the repair by ligation but is without effect on the recombination.

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Rejoining of DNA double‐strand breaks in human fibroblasts and its impairment in one ataxia telangiectasia and two fanconi strains

Coquerelle¹,

Weibezahn²

1981

J. Supramol. Struct. Cell. Biochem.

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Using the technique of neutral elution through polycarbonate filters as a measure of DNA length, and hence of the number of double-strand breaks incurred as a result of radiation damage, we found that normal human fibroblasts rejoin 50% of all breaks within only 3 min (37 degrees C). This fast rejoining was impaired in fibroblasts from one patient with Ataxia telangiectasia and in fibroblasts from two patients with Fanconi's anemia. Also the number of residual breaks after several hours of repair was higher than in control cells. Other cases with the same diseases were normal in their rejoining of double-strand breaks.

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Negative Pion Irradiation of Mammalian Cells

Cited by 17 publications

References 9 publications

Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes

Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes

Promotion of double-strand break repair by human nuclear extracts preferentially involves recombination with intact homologous DNA

Rejoining of DNA double‐strand breaks in human fibroblasts and its impairment in one ataxia telangiectasia and two fanconi strains

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