Needle volatiles as chemophenetic markers in differentiation of natural populations of Abies alba, A. x borisii-regis, and A. cephalonica

Nikolić, Jelena S.; Zlаtković, Bojаn; Jovanović, Snežana Č.; Stojanović, Gordana; Marin, Petar D.; Mitić, Zorica S.

doi:10.1016/j.phytochem.2020.112612

Cited by 16 publications

(19 citation statements)

References 19 publications

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“…Additionally, detailed chemotyping of A. alba , A . × borisii‐regis , and A. cephalonica populations at the level of headspace volatile compounds was performed by Nikolić et al [3] …”

Section: Resultsmentioning

confidence: 99%

“…Additionally, detailed chemotyping of A. alba, A. × borisii-regis, and A. cephalonica populations at the level of headspace volatile compounds was performed by Nikolić et al [3] Antimicrobial Activity Antimicrobial activities of the three Abies EOs were evaluated by microdilution method against 17 investigated strains (ATCC and respiratory isolates) of several species pathogenic to humans. The minimum inhibitory concentrations (MICs) of tested EOs samples varied from 0.62 to 20.00 mg/mL (Table 2).…”

Section: Abies Eos Yields and Chemical Compositionsmentioning

confidence: 99%

“…(King Boris fir) was reported as a natural hybrid between A. alba and A. cephalonica , with several either intermediate or mosaic of morphological characteristics of supposed parent species [2] . Nevertheless, its taxonomical status, origin and distribution have not been entirely resolved [3] . Based on the present‐day floristic databases, A .…”

Section: Introductionmentioning

confidence: 99%

“…[2] Nevertheless, its taxonomical status, origin and distribution have not been entirely resolved. [3] Based on the present-day floristic databases, A. × borisii-regis or A. borisii-regis is considered a distinct species.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

Mitić

Stojanović-Radić

Jovanović

et al. 2022

Chemistry & Biodiversity

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This study focused on the essential oils (EOs) isolated from needles with twigs of three indigenous Balkan Abies species (A. alba, A. × borisii‐regis and A. cephalonica) regarding their chemical composition, antimicrobial activity and toxicity toward crustaceans and insects. Even though distinct phytochemical profiles of dominant volatiles were revealed for each species, β‐pinene and α‐pinene represented the first two major volatiles in all three EOs. Antimicrobial activity of EOs has shown inhibitory effect against all 17 studied strains (ATCC and respiratory isolates) in the range of 0.62–20.00 mg/mL (MICs). Further, all three EOs exhibited strong toxicity (LC50<100 μg/mL) in Artemia salina lethality bioassay, but with significant differences that depended on the EO type. Additionally, tested EOs have shown a certain level of toxicity against Drosophila melanogaster, mostly at the highest tested concentration (3 %) which caused significant prolongation of developmental time, larvicidal effect and pupal mortality. In the three biological assays performed, there was no observed inhibitory effect or weakest activity for A. alba EO. Further, A. cephalonica EO has shown the highest levels of antimicrobial activity and toxicity toward A. salina, while in relation to the insecticidal potential, A. cephalonica and A. × borisii‐regis EOs exhibited similar level of toxicity against D. melanogaster.

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“…Additionally, detailed chemotyping of A. alba , A . × borisii‐regis , and A. cephalonica populations at the level of headspace volatile compounds was performed by Nikolić et al [3] …”

Section: Resultsmentioning

confidence: 99%

Section: Abies Eos Yields and Chemical Compositionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

Mitić

Stojanović-Radić

Jovanović

et al. 2022

Chemistry & Biodiversity

Self Cite

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“…The headspace sampling conditions were as described by Nikolić et al [60]. A total of 150 mg (per sample) of fresh plant material, comprising in vitro plantlets collected at the end of 4-week subculture, was used.…”

Section: Headspace Isolationmentioning

confidence: 99%

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Stojičić

Tošić

Stojanović

et al. 2022

Plants

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Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography–mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.

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Cuticular Wax Variability of Abies alba, A.×borisii‐regis and A. cephalonica from the Balkans: Chemophenetic and Ecological Aspects

Mitić

Nikolić

Dimitrijevic

et al. 2023

Chemistry & Biodiversity

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This is the first study on cuticular wax variability of Abies alba, A. × borisii-regis and A. cephalonica, using 18 native populations from the assumed hybrid zone in the Balkan Peninsula. Presence of 13 n-alkanes with chain-lengths ranging from C 21 to C 33 , one primary alcohol, two diterpenes, one triterpene and one sterol was determined in hexane extracts of 269 needle samples. The multivariate statistical analyses at the population level entirely failed in supporting circumscription of Balkan Abies taxa and therefore, in identifying hybrid populations.However, performed at the species level, these analyses revealed a certain tendency of differentiation between A. alba and A. cephalonica, while individuals of A. × borisii-regis were largely overlapped by the clouds of both parent species. Finally, the correlation analysis suggested that the observed variation of wax compounds was probably genetically conditioned and that it does not represent an adaptive response to various environmental factors.

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Needle volatiles as chemophenetic markers in differentiation of natural populations of Abies alba, A. x borisii-regis, and A. cephalonica

Cited by 16 publications

References 19 publications

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Cuticular Wax Variability of Abies alba, A.×borisii‐regis and A. cephalonica from the Balkans: Chemophenetic and Ecological Aspects

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