Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Abstract

Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography–mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.

Clinopodium L. Taxa from the Balkans—Are There Unique Leaf Micromorphological and Phytochemical Patterns?

“…Still, these types of trichomes, which are considered a taxonomical characteristic, are mainly present only in floral regions. The present analysis of leaves from the section Pseudomellisa (i.e., Pseudomellisa -group) showed results in agreement with the studies by Kremer [ 50 ] and Stojičić [ 52 ], with the most abundant C1a subtype of trichomes on the leaves. The population of C. pulegium from Podvis, included in the present research, contained one additional type of capitate trichome not noticed in the Kremer study (Međeđa population) [ 50 ].…”