Necrotizing Pancreatitis and Gizzard Erosion Associated with Adenovirus Infection in Chickens

Tanimura, Nobuhiko; Nakamura, Kikuyasu; Imai, Kunitoshi; Maeda, Masako; Gobo, T; Nitta, Sayuri; Ishihara, T; Amano, Hodaka

doi:10.2307/1591697

Cited by 49 publications

(35 citation statements)

References 9 publications

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“…However, so far, gizzard erosions have been mostly found in slaughtered broiler chickens, and there have been few reports of gizzard erosions in layer chickens. 25 The broiler chickens with gizzard erosion attributable to experimentally induced infection manifested clinical signs of depression and anorexia, 16 and mortality of commercial broiler chicks with gizzard erosion has been reported. 2 In this study, as well as in a previous study by the authors, 15 however, SPF White Leghorn chickens inoculated with 99ZH did not develop clinical signs of disease despite the fact that they harbored gizzard erosions.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

et al. 2006

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Abstract. The fiber gene sequence and pathogenicity of the serotype-1 fowl adenovirus (FAdV-1) isolated from gizzard erosions and from clinically normal chickens were compared among isolates. The FAdV-99ZH strain, which induced gizzard erosions, had a nucleotide sequence of the long fiber gene that was different from that of the Ote strain, which did not induce gizzard erosions. The differences could be distinguished by use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The long fiber gene of 16 FAdV-1 isolates from gizzard erosions and 10 FAdV-1 isolates from the feces of clinically normal chickens was examined by use of PCR-RFLP analysis. All 16 FAdV-1 isolates from gizzard erosions had the same restriction patterns as those of strain 99ZH; however, 10 FAdV-1 isolates from normal chickens were classified into 3 groups. Specific-pathogen-free (SPF) chickens were inoculated orally with 2 FAdV-1 isolates from gizzard erosions or 3 FAdV-1 isolates from clinically normal chickens to determine the pathogenicity of each strain. Two of 2 FAdV-1 isolates from gizzard erosions induced gizzard erosions. Two of 3 FAdV-1 isolates from normal chickens had the same PCR-RFLP patterns as those of the Ote strain, but did not induce any gizzard erosions. However, 1 FAdV-1 isolate from clinically normal chickens had the same PCR-RFLP pattern as that of strain 99ZH and induced gizzard erosions. These results indicate that there are FAdV-1 strains that have different pathogenicity; one strain induces gizzard erosions, and the other does not. Use of PCR-RFLP analysis of long fiber genes may be able to distinguish between these two strains.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

et al. 2006

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“…Gizzard erosion associated with FAV in layer chickens, broiler chickens, and quails has been reported [2,5,13,17]. It has been reported that many outbreaks of gizzard erosion associated with FAV occurred frequently in Japanese slaughterhouses and most of FAV isolated from eroded gizzard were of serotype 1 [14].…”

Section: Discussionmentioning

confidence: 99%

“…In the field, gizzard erosion associated with serotype 8 FAV has also been reported. Tanimura et al [17] reported a field cace of gizzard erosion in which intranuclear inclusion bodies were seen in necrotic epithelial cells of the gizzard, and seroptype 8 FAV was isolated from liver samples. In this case, because chicken anemia virus (CAV) was isolated as well as serotype 8 FAV, it was deduced that CAV might have contributed to the outbreak.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Serotype 8 Fowl Adenovirus Isolated from Gizzard Erosions of Slaughtered Broiler Chickens

Okuda

Ono

Shibata

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The pathogenicity of serotype 8 fowl adenovirus (FAV), isolated from gizzard erosions of slaughtered broiler chickens, was investigated. In experiment 1, 29 5-day-old specific-pathogen-free (SPF) chickens were inoculated with the isolates of serotype 8 FAV, M013 (group 1) or G0054 (group 2) strain, via an oral route. There were no clinical signs in any of chickens after inoculation, and mild gizzard erosions were observed macroscopically and microscopically in three inoculated chickens of group 2. FAV was recovered f rom gizzards and rectums but was not recovered from pancreas and livers from chickens in both inoculated groups. In experiment 2, 27 1-day-old SPF chickens were inoculated with the G0054 strain by intramuscular route. Five, 6, and 3 inoculated chickens died on days 3, 4, and 5 postinoculation (PI), respectively. Four, 3, 1, and 1 inoculated chickens became moribund with severe clinical sign s such as ruffled feathers, severe depression and closed eyes from days 3 to 6 PI, respectively. Macroscopically, the common characterist ic of the gross lesions of dead chickens and euthanized moribund chickens was discoloration of liver. FAV was recovered from the gizzard, liver, pancreas and rectum. Virus titers in the liver and pancreas were high until day 6 PI. Histologically, necrotizing hepatitis an d pancreatitis with intranuclear inclusion bodies were observed in the inoculated chickens. These results indicate that some strains of seroty pe 8 FAV are able to reproduce not only gizzard erosion by oral inoculation but inclusion body hepatitis (IBH) by intramuscular inoculation. KEY WORDS: gizzard erosion, inclusion body hepatitis, serotype 8 fowl adenovirus.J. Vet. Med. Sci. 66(12): 1561-1566, 2004 The pathogenicity of serotype 8 fowl adenoviruis (FAV) has been reported in field cases of inclusion body hepatitis (IBH) in chickens [7] as well as in experimental reproduction of IBH in specific-pathogen-free (SPF) chickens [6]. Recently, serotype 8 FAV was isolated from gizzards exhibiting gizzard erosion in slaughterhouses [14]. In pervious reports, however, gizzard erosion was reproduced in SPF white leghorn chickens [9,11] and commercial broiler chickens [12] inoculated with serotype 1 FAV isolated from the gizzards of commercial broiler chickens exhibiting gizzard erosion. Moreover, most cases of gizzard erosion that have occurred frequently in slaughterhouses in Japan were caused by serotype 1 FAV infection [14]. Therefore, it was not clear whether or not serotype 8 FAV was the cause of gizzard erosion.The purpose of this study was to investigate the pathogenicity of serotype 8 FAV isolated from eroded gizzard by inoculating SPF chickens with the isolates via oral and intramuscluar routes. MATERIALS AND METHODSVirus: Two isolates of FAV, isolated from gizzard erosions of slaughtered broiler chickens, were cloned three times by means of the terminal dilution method and designated as the M013 and G0054 strains. These strains were propagated in primary chicken kidney (CK) cells and used for chicke...

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“…Recently, the gizzard erosions and ulcers in chickens were observed in Japan. FAV serotypes 1 and 8 were isolated from the affected gizzards [2,15,17]. Gizzard erosion was experimentally reproduced in chickens inoculated with isolated FAV serotype 1 [10][11][12][13][14]18].…”

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confidence: 99%

Putative Host Cell Receptor for Fowl Adenovirus Detected in Gizzard

Taharaguchi

Kono

Ohta

et al. 2007

The Journal of Veterinary Medical Science

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Necrotizing Pancreatitis and Gizzard Erosion Associated with Adenovirus Infection in Chickens

Cited by 49 publications

References 9 publications

Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

Pathogenicity of Serotype 8 Fowl Adenovirus Isolated from Gizzard Erosions of Slaughtered Broiler Chickens

Putative Host Cell Receptor for Fowl Adenovirus Detected in Gizzard

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