Putative Host Cell Receptor for Fowl Adenovirus Detected in Gizzard

Taharaguchi, Satoshi; Kono, Yukiharu; Ohta, Hikoto; Takase, Kozo

doi:10.1292/jvms.69.1203

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…Pathological changes and detection of the virus in the gastrointestinal system, particularly in the gizzard, in the course of a FAdV-1 infection are contrary to other documented FAdV infections, such as inclusion body hepatitis and hydropericardium/hepatitis syndrome, both targeting the liver with corresponding tissue destruction [18]. A putative FAdV-1 cell receptor in gizzard mucosal cells, described by Taharaguchi et al [19], may explain this tissue tropism of virulent FAdV-1.…”

Section: Discussionmentioning

confidence: 99%

Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Grafl

Liebhart

Günes

et al. 2013

Vet Res

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In the present study day-old specific-pathogen-free (SPF) and commercial broilers with maternally derived fowl adenovirus serotype 1 (FAdV-1) antibodies were orally infected with a European “pathogenic” FAdV-1, isolated from broilers showing signs of gizzard erosion. During the experiment, broilers were observed and weighed daily up to 17 days post infection (dpi). Clinically, both infected groups showed significant decrease of weight compared to respective negative control groups. Birds were examined by necropsy at 3, 7, 10, 14 and 17 dpi. Pathological changes in the gizzards were noticed in both experimentally infected groups from 7 dpi onwards. Macroscopically, erosion of the koilin layer and inflammation or ulceration of the gizzard mucosa were observed. Histologically, presence of FAdV-1 in intranuclear inclusion bodies of degenerated glandular epithelial cells was demonstrated by in-situ hybridization and inflammatory cell infiltration of the lamina propria, submucosa and muscle layer was detected. Tissue samples were investigated by a recently developed real-time PCR and the viral DNA load was calculated from gizzard, liver, spleen and cloacal swabs with the highest amounts of FAdV-1 DNA found in the gizzard. For the first time, successful reproduction of clinical signs in broilers as well as pathological lesions in the gizzard were achieved with a European FAdV-1 isolate displaying some genetic differences to so far reported virulent FAdV-1 from Japan. Furthermore, highest viral load in gizzards could be linked with macroscopical and histological lesions. Therefore, the conducted analyses provide important insights into the pathogenesis of adenoviral gizzard erosion.

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Section: Discussionmentioning

confidence: 99%

Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Grafl

Liebhart

Günes

et al. 2013

Vet Res

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“…Adenoviruses could bind to different molecules as coxsackievirus and adenovirus receptor, integrins, MHC class I particles, proteoglycans, and others undetermined as receptor in the lungs and nervous system (Hong et al, 1997;Nemerow and Stewart, 1999;Zabner et al, 1999;Dechecchi et al, 2000;Tan et al, 2001). The protein of 200 kDa in the membrane fraction of a cell in a gizzard was identified as the binding target for the FAdV JM 1/1 strain isolated from gizzard erosion in chicken (Taharaguchi et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

et al. 2011

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Broiler chickens with clinical signs of uneven growth, depression, and dull feathers were submitted to our laboratory and, at necropsy, lesions in proventriculus, gizzard, and intestines were detected. Fowl adenovirus serotype 1 (FAdV-1) was isolated from digestive tissues. The virus, assigned as FAdV-PL/G068/08, showed 99.5% nucleotide homology and 99.2% amino acid homology in hexon gene with chicken embryo lethal orphan (CELO) strain classified as the European reference of FAdV-1. One-day-old and 21-d-old SPF chickens were inoculated with FAdV-PL/068/08 by both nasal and ocular routes and then observed daily and examined by necropsy at 6, 10, and 14 d postinoculation. Experimental infection with isolated virus was fatal for younger chickens and major lesions occurred in the gizzards. No clinical or pathological changes were observed in chickens infected at 21 d of age, but the presence of intranuclear inclusion bodies in gizzard epithelial cells was detected. Molecular characterization was based on the long and short fibers genes sequencing and comparison of obtained sequences with other FAdV-1 strains. The homology between FAdV-PL/G068/08 and other sequences available in GenBank was between 98.9 and 99.8% (short fiber region) and 99.0 and 99.7% (long fiber region) at nucleotide level and between 98.4 and 100% (short fiber region) and 99.3 and 99.9% (long fiber region) at amino acid level. No correlation between identified amino acid changes in short and long fiber proteins and pathogenicity of studied FAdV-1 strains was observed. Although short and long proteins were indicated as factors influencing virus pathogenicity, the role of identified sequence differences in infectivity determination remain unclear.

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“…Viral overlay protein binding assay (VOPBA) has been used to identify receptor proteins for various viruses, such as Bovine adenovirus [8], Dengue Virus [9], Fowl Adenovirus [10], and pancreatic necrosis virus [11]. This assay is sensitive enough to detect the binding of less than 100 mg of protein in a crude membrane preparation or to detect 5 mg of purified glycophorin [12].…”

Section: Introductionmentioning

confidence: 99%

Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells

Zhang

Hua

Shen

et al. 2011

Virol J

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Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cross-species infection of HEV from swine to humans is still unclear. This study sought to identify receptor element for genotype 4 HEV on swine liver cells using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single virus binding band with natural molecular weight about 55 kDa was observed, and mass spectrometry revealed that this virus binding band contained 31 different proteins. Infection inhibition assay suggested that this 55 kDa protein could prevent HEV from infecting its susceptible A549 cell line, which was further confirmed by the HEV genome detecting in the inoculated cells. Further research should be performed to elucidate the accurate receptor of HEV on the swine liver cells.

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Putative Host Cell Receptor for Fowl Adenovirus Detected in Gizzard

Cited by 6 publications

References 15 publications

Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers

Molecular characterization of fowl adenoviruses isolated from chickens with gizzard erosions

Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells

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