Necessity of regulatory guidelines for the development of amyloid based biomaterials

Kumar, Vijay; Sinha, Nabodita; Thakur, Ashwani Kumar

doi:10.1039/d1bm00059d

Cited by 7 publications

(9 citation statements)

References 164 publications

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“…Amyloids are considered as paradoxical because of their pathogenic as well as functional roles across diverse species. (40, 41) Despite plausible presence of amyloids in plants, the plant proteins investigated till now are often fibrillated at extreme conditions such as high temperature and therefore does not reflect on the behaviour of the proteins in physiological conditions. Moreover, no study has employed a combinatorial approach with latest probes such as Proteostat ® and the standard CR assay for amyloid detection in plants.…”

Section: Discussionmentioning

confidence: 99%

Protein reservoirs of seeds are composites of amyloid and amyloid-like structures facilitating sustained release during germination and seedling growth

Sinha

Gahane

Zahra

et al. 2021

Preprint

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Seed storage proteins, well-known for their nutritional functions are sequestered in protein bodies. However, their biophysical properties at the molecular level remain elusive. Based on the structure and function of protein bodies found in other organisms, we hypothesize that the seed protein bodies might be present as amyloid structures. When visualized with a molecular rotor Thioflavin-T and a recently discovered Proteostat probe with enhanced sensitivity, the seed sections showed amyloid-like signatures in the protein storage bodies of the aleurone cells of monocots and cotyledon cells of dicots. To make the study compliant for amyloid detection, gold-standard Congo red dye was used. Positive apple-green birefringence due to Congo red affinity in some of the areas of ThT and Proteostat binding, suggests the presence of both amyloid-like and amyloid deposits in the protein storage bodies. Further, diminishing amyloid signature in germinating seeds implies the degradation of these amyloid structures and their utilization. This study will open new research avenues for a detailed molecular-level understanding of the formation and utilization of aggregated protein bodies as well as their evolutionary roles.

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Section: Discussionmentioning

confidence: 99%

Protein reservoirs of seeds are composites of amyloid and amyloid-like structures facilitating sustained release during germination and seedling growth

Sinha

Gahane

Zahra

et al. 2021

Preprint

Self Cite

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“…Amyloid-based hydrogels are attractive and have great potential application in the biomaterials field scaffolds for tissue engineering, drug delivery, nano devices, and food technology [ 1 ]. Biomacromolecules can build a hydrogel scaffold directly under variation of temperature, pH, ionic force, and salt concentration.…”

Section: Protein Based Metamaterialsmentioning

confidence: 99%

“…Metamaterials exhibit physical properties such as negative electrical permittivity and magnetic susceptibility not obtainable from natural materials, thanks to their engineered microarchitecture [ 1 , 2 ]. These characteristics are derived from the material’s structural pattern rather than from its intrinsic properties [ 2 ], making metamaterials one of the most prominent types of engineered materials and allowing the production of highly tunable devices and sensors [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Of special interest are optical metamaterials that can alter electromagnetic waves at determined optical frequencies, conferring properties such as high transparency, high light absorbance, negative refractive index, hyperbolic dispersion and absorption of light at both a broad range of wavelengths in the electromagnetic spectrum or at precise wavelengths [ 1 , 3 , 4 , 5 , 6 , 7 ]. Proposed optical metamaterials applications include devices with integrated X-Ray or UV light sources, highly-sensitive sensors, cloaking, improved photovoltaic cells, and light-tunable control mechanisms for electromagnetic systems [ 1 , 4 , 5 , 6 , 7 ]. These devices could be applied in multiple areas such as lab-on-a-chip systems, microfluidics, point-of-care diagnostics, biological system sensing and regulation, health monitoring, light-controlled therapeutics, high-resolution and label-free imaging, drug delivery, and tissue engineering [ 3 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Biomolecule-Based Optical Metamaterials: Design and Applications

et al. 2022

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Metamaterials are broadly defined as artificial, electromagnetically homogeneous structures that exhibit unusual physical properties that are not present in nature. They possess extraordinary capabilities to bend electromagnetic waves. Their size, shape and composition can be engineered to modify their characteristics, such as iridescence, color shift, absorbance at different wavelengths, etc., and harness them as biosensors. Metamaterial construction from biological sources such as carbohydrates, proteins and nucleic acids represents a low-cost alternative, rendering high quantities and yields. In addition, the malleability of these biomaterials makes it possible to fabricate an endless number of structured materials such as composited nanoparticles, biofilms, nanofibers, quantum dots, and many others, with very specific, invaluable and tremendously useful optical characteristics. The intrinsic characteristics observed in biomaterials make them suitable for biomedical applications. This review addresses the optical characteristics of metamaterials obtained from the major macromolecules found in nature: carbohydrates, proteins and DNA, highlighting their biosensor field use, and pointing out their physical properties and production paths.

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Enzyme‐Instructed Self‐Assembly of Peptides: From Concept to Representative Applications

Kim

2022

Chemistry An Asian Journal

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Enzyme‐instructed self‐assembly, integrating enzymatic reaction and molecular self‐assembly, has drawn noticeable attention over the last decade with the intension of being used in valuable applications. Recent advances in the field make it possible to spatiotemporally control peptide self‐assembly in cellular milieu, broadening the potential applications of peptide assemblies to cancer therapy and subcellular delivery. In this review, the concept of enzyme‐instructed self‐assembly of peptide, containing enzymatic trigger and spatiotemporal control, is described. Representative applications in cells are also discussed, followed by an outlook on the field of enzyme‐instructed self‐assembly.

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Necessity of regulatory guidelines for the development of amyloid based biomaterials

Abstract: The recent surge in amyloid-based biomaterial development needs regulatory guidelines and safety assessment to avoid adverse health effects.

Cited by 7 publications

References 164 publications

Protein reservoirs of seeds are composites of amyloid and amyloid-like structures facilitating sustained release during germination and seedling growth

Protein reservoirs of seeds are composites of amyloid and amyloid-like structures facilitating sustained release during germination and seedling growth

Biomolecule-Based Optical Metamaterials: Design and Applications

Enzyme‐Instructed Self‐Assembly of Peptides: From Concept to Representative Applications

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