Near-Infrared Upconversion Controls Photocaged Cell Adhesion

Abstract

Dynamic control of cell-surface interactions with near-infrared (NIR) light is particularly attractive for regeneration medicine and cell-based therapy. Herein we successfully achieve NIR-controlled cell adhesion with upconversion nanoparticles (UCNPs) based programmable substrate. The UCNPs can harvest the biocompatible NIR light and convert it into local UV light, which results in cleavage of the photocaged linkers and on-demand release of adhesive cells. The strategy also enables the feasibility of deep-tissue photocontrol of cell adhesion on substrate. Our work may open a new avenue for design of UCNP-based cell scaffolds to dynamically manipulate cell-matrix and cell-cell interactions.

Lanthanide Nanoparticles: From Design toward Bioimaging and Therapy

“…Using a 2-nitrobenzyl contained photoresponsive linker between the cell and the silica coated upconverting nanorod, the "on demand" release of cells can be realized by simply applying NIR in-deep tissue irradiation. 512 This novel demonstration of NIR upconver- Among all of the applications of the NIR upconversionmediated photolysis of ortho-nitrobenzyl for biomedical interest, an in vivo study was carried out by Xing et al 513 The designed conjugates of D-luciferin/hexagonal NaY-F 4 :Yb,Tm@NaYF 4 @SiO 2 release the caged D-luciferin, which can recognize firefly luciferase reporter genes and give bioluminescence at 560 nm when excited by 980 nm NIR light. 4.8.2.1.2.…”

Antigen-Loaded Upconversion Nanoparticles for Dendritic Cell Stimulation, Tracking, and Vaccination in Dendritic Cell-Based Immunotherapy

“…[18][19][20][21][22][23] Distinguished from conventional down-conversion photoluminescent probes, UCNPs could convert two or more low-energy photons to a single high-energy output photon under NIR excitation in the progress of upconversion luminescence (UCL). Therefore, UCNPs have many advantages in imaging such as minimal autofluorescence background signals and high resistance to photobleaching, which make them suitable for sensitive detection and imaging in complicated biological samples.…”

Instructing cells with programmable peptide DNA hybrids

“…Previous studies have used strategies like enzymatic cleavage5, thermoresponsive polymers18, electroswitching19, photocleavage4202122, photoisomerization20 or host-guest displacement67 to dynamically control cell adhesion. Most of these approaches, however, are limited to one bioactive signal and rarely offer reversibility over multiple cycles.…”