Near-infrared laser mediated modulation of ice crystallization by two-dimensional nanosheets enables high-survival recovery of biological cells from cryogenic temperatures

Abstract

Two-dimensional (2D) graphene oxide (GO) and molybdenum disulfide (MoS2) nanosheets (NSs) have been widely used as photothermal agents and as potential carriers of antitumor drugs. Their spatial thermal effects have been extensively explored for use at physiological and hyperthermic temperatures (37 to 46 °C). Furthermore, the modulation of the spatial thermal distributions with these NSs may have even more profound applications in the microstructural control of biomaterials at cryogenic temperatures (-196 to 37 °C). These applications include bioinspired microfabrication via freezing, food and drug freeze-drying, and biomaterial cryopreservation. However, such thermal effects of NSs and their applications at cryogenic temperatures had never been fully explored. Therefore, in this study, we have utilized the near-infrared laser induced photothermal effects of GO and MoS2 NSs to suppress the ice nucleation and ice crystal growth during warming of the biosamples. Using this approach, biological cells subjected to fast cooling to a deeply frozen state (-196 °C) were successfully recovered with high survival rates and full biological functionality. Thus, we provide a NS based effective approach to control the crystallization behaviors of water during warming at cryogenic temperatures, as NSs may have wide applications in both materials science and bioengineering.

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

“…Reproduced with permission. [ 178 ] Copyright 2018, Royal Society of Chemistry. F) The temperature trend of vitrification solution with and without liquid nanoparticles in warming process.…”

Multifunctional Photo- and Magnetoresponsive Graphene Oxide–Fe3O4 Nanocomposite–Alginate Hydrogel Platform for Ice Recrystallization Inhibition

“…Furthermore, an alternative novel strategy for suppressing IR is improving the warming rate to decrease the formation of ice crystals during rewarming. Heating induced by NIR light (Figure c) or AMF (Figure d) can be employed to assist warming, thereby suppressing IR. , In the study, we developed an all-in-one platform for controlling ice recrystallization by functional materials and external physical fields (Figure e).…”

“…The small amount of backscattering off the surface of the GO–Fe 3 O 4 -containing solution is comparable to that of deionized water. However, the solution with carbon particles has photons mainly absorbed by particles near the solution surface . To further evaluate the photothermal and magnetothermal effects, the temperature increase of CPAs with GO–Fe 3 O 4 NCs was measured from 25 °C for 1 min under NIR and AMF.…”

Advanced Biotechnology for Cell Cryopreservation

“…It has promoted long-term storage of cell resources and banking "off-the-shelf" cell-based therapy products at large scale. Since hydrogel capsules supplement excellent cryopreservation, satisfactory outcomes of cell cryopreservation can be also achieved at reduced DMSO concentrations [59,102,103]. Droplet-based cell printing can improve cell vitrification and can be helpful to achieve high efficiency at low-CPA concentration and reduce CPA toxicity and osmotic stress during CPA loading and unloading process [86].…”